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Q3KHB4

- GLND_PSEPF

UniProt

Q3KHB4 - GLND_PSEPF

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, Pfl01_1099
Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPFLU205922:GJBD-1118-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Pfl01_1099
OrganismiPseudomonas fluorescens (strain Pf0-1)
Taxonomic identifieri205922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002704: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_0000231686Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi205922.Pfl01_1099.

Structurei

3D structure databases

ProteinModelPortaliQ3KHB4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini486 – 590105HDAdd
BLAST
Domaini706 – 78984ACT 1Add
BLAST
Domaini816 – 90085ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseUniRule annotationAdd
BLAST
Regioni343 – 705363Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3KHB4-1 [UniParc]FASTAAdd to Basket

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MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRQAREVLD ARFRAGRDIR    50
RLIEDRAWFV DNILQKAWDQ FDWSEDADIA LVAVGGYGRG ELHPYSDIDL 100
LILLDSADHE IFRDSIERFL TLLWDIGLEV GQSVRSVDEC AEEARADLTV 150
VTNLMESRTI CGPERLRQRM LDVTSTAHMW PSKDFFLAKR AEQKARHHKY 200
NDTEYNLEPN VKGSPGGLRD IQTILWVARR QYGTLNLRAL AGEGFLVESE 250
NALLASSQEF LWKVRYALHM LAGRSEDRLL FDHQRTIAGL LGFEGDDAKQ 300
AVENFMQQYF RVVMSIAQLS DLIIQHFEEV ILAPEDEAPP QPINSRFQLH 350
DGYIEARNDN VFRRTPFAML EIFVLMAQQP EIKGVRADTI RLLRENRHLI 400
DDNFRNDIRN TSLFIELFKC KIGIHRNLRR MNRYGILGRY LPEFGFIVGQ 450
MQHDLFHIYT VDAHTLNLIK HLRKLQYTQV SEKFPLAAKL MAKLPKPELI 500
YMAGLYHDIG KGRHGDHSEI GAVDAEAFCQ RHQLPVWDSR LIVWLVQNHL 550
VMSTTAQRKD LSDPQVIHDF ALAVGDETRL DYLYVLTVAD INATNPTLWN 600
SWRASLLRQL YTETKRALRR GLENPVDREE QIRQTQSAAL DILVRGGTDP 650
DDVEQLWAQL GDDYFLRHTA GDVAWHTDAI LQQPADGGPL VLIKETTQRE 700
FEGGTQIFIY APDQHDFFAV TVAAMDQLNL NIHDARVITS SSQFTLDTYI 750
VLDTDGDSIG DNPVRVKQIR DGLTEALRNP ADYPTIIQRR VPRQLKHFAF 800
APQVTIHNDA QRPVTVLELT APDRPGLLAR VGGIFLEFDL SLQNAKIATL 850
GERVEDVFFI TDAHNQPLSD PLLCSRLQDA IVEQLSVNQE PDIKLSRISI 900
Length:900
Mass (Da):102,820
Last modified:November 8, 2005 - v1
Checksum:iB21FDACD3FA1D1A0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000094 Genomic DNA. Translation: ABA72842.1.
RefSeqiYP_346831.1. NC_007492.2.

Genome annotation databases

EnsemblBacteriaiABA72842; ABA72842; Pfl01_1099.
GeneIDi3716504.
KEGGipfo:Pfl01_1099.
PATRICi19884289. VBIPseFlu44242_1121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000094 Genomic DNA. Translation: ABA72842.1 .
RefSeqi YP_346831.1. NC_007492.2.

3D structure databases

ProteinModelPortali Q3KHB4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 205922.Pfl01_1099.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA72842 ; ABA72842 ; Pfl01_1099 .
GeneIDi 3716504.
KEGGi pfo:Pfl01_1099.
PATRICi 19884289. VBIPseFlu44242_1121.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PFLU205922:GJBD-1118-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf0-1.

Entry informationi

Entry nameiGLND_PSEPF
AccessioniPrimary (citable) accession number: Q3KHB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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