Q3KE79 (GLO2_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Pfl01_2184

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas fluorescens

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP-Rule MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP-Rule MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP-Rule MF_01374
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutathione biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Hydroxyacylglutathione hydrolase HAMAP-Rule MF_01374		PRO_0000309684
Sites
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 1 By similarity
	Metal binding	60	1	Zinc 2 By similarity
	Metal binding	61	1	Zinc 2 By similarity
	Metal binding	112	1	Zinc 1 By similarity
	Metal binding	129	1	Zinc 1 By similarity
	Metal binding	129	1	Zinc 2 By similarity
	Metal binding	167	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3KE79 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: BDCD6DC82379EA44	FASTA	255	28,126
	10 20 30 40 50 60 MIQISALPAF TDNYIWLLQD HATQRCAVVD PGDAAPVQAW LDAHPGWVLG DILITHHHHD 70 80 90 100 110 120 HVGGVETLKK ATGATVYGPA SENIPGRDVA LKDNDTVRVL GWDFDVYAVP GHTLGHIAYY 130 140 150 160 170 180 HHGLLFCGDT LFAAGCGRLF EGTPQQMHQS LSRLAALPED TLVYCTHEYT LSNLKFAAAV 190 200 210 220 230 240 EPDNRDIAAR LEKVTRQREN GVMTLPSTLA LEKLTNPFLR TGEILVTQKV DERNGPQNRA 250 PSEVFAALRA WKDTF

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf0-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA73927.1.
RefSeq	YP_347916.1. NC_007492.2.
3D structure databases
ProteinModelPortal	Q3KE79.
ModBase	Search...
Protein-protein interaction databases
STRING	205922.Pfl01_2184.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABA73927; ABA73927; Pfl01_2184.
GeneID	3715725.
KEGG	pfo:Pfl01_2184.
PATRIC	19886519. VBIPseFlu44242_2218.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HOG000058041.
KO	K01069.
OMA	LTHHHQD.
ProtClustDB	CLSK866802.
Enzyme and pathway databases
BioCyc	PFLU205922:GJBD-2223-MONOMER.
UniPathway	UPA00619; UER00676.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2.
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
PIRSF	PIRSF005457. Glx. 1 hit.
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_PSEPF

Accession

Primary (citable) accession number: Q3KE79

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program