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Protein

Catalase-peroxidase

Gene

katG

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei88 – 881Transition state stabilizerUniRule annotation
Active sitei92 – 921Proton acceptorUniRule annotation
Metal bindingi286 – 2861Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciPFLU205922:GJBD-2240-MONOMER.

Protein family/group databases

PeroxiBasei2315. PfCP01_PfO1.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:Pfl01_2201
OrganismiPseudomonas fluorescens (strain Pf0-1)
Taxonomic identifieri205922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002704 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 755755Catalase-peroxidasePRO_0000354864Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki91 ↔ 245Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-271)UniRule annotation
Cross-linki245 ↔ 271Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiQ3KE62.

2D gel databases

World-2DPAGE0008:Q3KE62.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi205922.Pfl01_2201.

Structurei

3D structure databases

ProteinModelPortaliQ3KE62.
SMRiQ3KE62. Positions 16-753.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiTESKCPF.
OrthoDBiEOG6RRKKM.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 3 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3KE62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANESKCPFN HAAGGGTTNR DWWPNQLNLK ILSQHSPASD PMGKDFDYAK
60 70 80 90 100
AFKSLDFQAL KRDLTALMTD SQEWWPADFG HYGPLFIRMA WHSAGTYRTA
110 120 130 140 150
DGRGGAGSGQ QRFAPLNSWP DNVSLDKARR LLWPIKQKYG RNISWADLIV
160 170 180 190 200
LTGNVALESM GFKTFGFSGG RADVWEPDED VYWGSENKWL GGDNRYGKDP
210 220 230 240 250
ESMQPPGEGT LVAEPAEHGN EESRTNQGER NLENPLAAVQ MGLIYVNPEG
260 270 280 290 300
PEGNPDPVAS AKDIRETFGR MAMNDEETVA LIAGGHAFGK THGAGPADNV
310 320 330 340 350
GPEPEAAGLE EQGLGWKNSF GTGKGADTIT SGLEVTWTTT PTKWSNNYLE
360 370 380 390 400
NLFGFEWELT KSPAGAHQWK PKNGAGAGTI PHAHDPNKRI DPTMLTSDLA
410 420 430 440 450
LRFDPTYEKI SRRFLANPDQ LADAFARAWY KLIHRDMGPL SRYLGPELPQ
460 470 480 490 500
EELLWQDPIP EATHPLVDDS DIRALKGKIL ASGLSVSELV STAWAAASTF
510 520 530 540 550
RGSDKRGGAN GGRLRLAPQK FWQANQPEQL DKVLKVLEGI QNEFNAGAAG
560 570 580 590 600
KQVSLADLIV LAGNAGVEQA AQNAGHTVTV PFNPGRTDAT QEQTDVESFG
610 620 630 640 650
FLEPHTDGFR NYAASTYRVP AEALLIDKAQ LLTLSAPEMT VLIGGLRVLD
660 670 680 690 700
TNVGKTQHGV FTDRPGTLTN DFFRNLLDMG VEWKPTSKTE FEGRDRKTGS
710 720 730 740 750
VKWTATRVDL VFGSNSVLRA LAEVYASSDA KEQFVTDFVA AWAKVMDLDR

FDLRQ
Length:755
Mass (Da):82,734
Last modified:November 8, 2005 - v1
Checksum:i33E6BD851C288542
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA. Translation: ABA73944.1.
RefSeqiWP_011333629.1. NC_007492.2.

Genome annotation databases

EnsemblBacteriaiABA73944; ABA73944; Pfl01_2201.
KEGGipfo:Pfl01_2201.
PATRICi19886553. VBIPseFlu44242_2235.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA. Translation: ABA73944.1.
RefSeqiWP_011333629.1. NC_007492.2.

3D structure databases

ProteinModelPortaliQ3KE62.
SMRiQ3KE62. Positions 16-753.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi205922.Pfl01_2201.

Protein family/group databases

PeroxiBasei2315. PfCP01_PfO1.

2D gel databases

World-2DPAGE0008:Q3KE62.

Proteomic databases

PRIDEiQ3KE62.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA73944; ABA73944; Pfl01_2201.
KEGGipfo:Pfl01_2201.
PATRICi19886553. VBIPseFlu44242_2235.

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiTESKCPF.
OrthoDBiEOG6RRKKM.

Enzyme and pathway databases

BioCyciPFLU205922:GJBD-2240-MONOMER.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 3 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf0-1.

Entry informationi

Entry nameiKATG_PSEPF
AccessioniPrimary (citable) accession number: Q3KE62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 8, 2005
Last modified: December 9, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.