Q3KDW0 (XYLA_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xylose isomerase
EC=5.3.1.5

Gene names

Name:	xylA
Ordered Locus Names:	Pfl01_2303

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-xylose = D-xylulose. HAMAP MF_00455
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP MF_00455
Subunit structure	Homotetramer By similarity. HAMAP MF_00455
Subcellular location	Cytoplasm By similarity HAMAP MF_00455.
Sequence similarities	Belongs to the xylose isomerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Pentose shunt Xylose metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW xylose isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Xylose isomerase HAMAP MF_00455		PRO_0000236966
Sites
	Active site	100	1	By similarity
	Active site	103	1	By similarity
	Metal binding	231	1	Magnesium 1 By similarity
	Metal binding	267	1	Magnesium 1 By similarity
	Metal binding	267	1	Magnesium 2 By similarity
	Metal binding	270	1	Magnesium 2 By similarity
	Metal binding	295	1	Magnesium 1 By similarity
	Metal binding	306	1	Magnesium 2 By similarity
	Metal binding	308	1	Magnesium 2 By similarity
	Metal binding	338	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3KDW0 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 79AE75129BD04827	FASTA	438	49,447
	10 20 30 40 50 60 MPYFPDIERI RYEGPASDSP LAFRHYDADK IILGKPMREH LRMAACYWHT FVWPGSDVFG 70 80 90 100 110 120 AGTFKRPWQH AGDPMELAIG KAQAAFEFFS KLGIDYYCFH DTDVAPEGNS LKEYRNHFAQ 130 140 150 160 170 180 MIDHLERHQE ESGIKLLWGT ANCFSNPRFA AGAASNPDPE VFACAAAQVF SAMNATQRLK 190 200 210 220 230 240 GANYVLWGGR EGYETLLNTD LKREREQLGR FMRMVVEHKY KIGFKGDLLI EPKPQEPTKH 250 260 270 280 290 300 QYDYDSATVF GFLQQFGLEK EIKVNIEANH ATLAGHSFHH EVATAVSLGI FGSIDANRGD 310 320 330 340 350 360 PQNGWDTDQF PNSVEEMTLV TYEILKAGGF GNGGFNFDSK VRRQSLDETD LFHGHVGAMD 370 380 390 400 410 420 VLALSLERAA AMVQNDQLQR LKDQRYAGWQ QPFGQSVLAG DFNLQSLAEH AFANELNPQA 430 VSGRQEMLEN VVNRFIYR

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf0-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA74046.1.
RefSeq	YP_348035.1. NC_007492.2.
3D structure databases
ProteinModelPortal	Q3KDW0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KDW0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3715586.
GenomeReviews	Gene locus Pfl01_2303 in contig CP000094_GR.
KEGG	pfo:Pfl01_2303.
PATRIC	19886767. VBIPseFlu44242_2337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2115.
HOGENOM	HBG297199.
OMA	LLGWDTD.
ProtClustDB	PRK05474.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_2303-MONOMER.
Family and domain databases
HAMAP	MF_00455. Xylose_isom_A. [Tree]
InterPro	IPR013022. Xyl_isomerase-like_TIM-brl. IPR012307. Xyl_isomerase_TIM-brl. IPR013452. Xylose_isom_bac. IPR001998. Xylose_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
KO	K01805.
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PRINTS	PR00688. XYLOSISMRASE.
SUPFAM	SSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.
TIGRFAMs	TIGR02630. Xylose_isom_A. 1 hit.
PROSITE	PS51415. XYLOSE_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLA_PSEPF

Accession

Primary (citable) accession number: Q3KDW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program