Reviewed, UniProtKB/Swiss-Prot Q3KDV1 (GLYA1_PSEPF)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 1
      Short name=Serine methylase 1
      Short name=SHMT 1
    EC=2.1.2.1

Gene names

Name:	glyA1
Ordered Locus Names:	Pfl01_2312

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Serine hydroxymethyltransferase 1 HAMAP MF_00051		PRO_0000235004
Amino acid modifications
	Modified residue	225	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3KDV1-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: BAB46C25DD93B93C	FASTA	412	45,462
	10 20 30 40 50 60 MSLQNFDPAI ARLIDRERNR QETHLELIAS ENYVSEEVLQ AQGSLLTNKY AEGYPGRRYY 70 80 90 100 110 120 GGCKVVDEIE NLAIERARKL FGCEYVNVQP HSGSQANQAV FLAVLEPGDR ILGMSLAHGG 130 140 150 160 170 180 HLTHGASVNF SGKFFQAFTY GLEKDSETLD YDQMEDLARE HRPKMIIAGA SAYSRVIDFP 190 200 210 220 230 240 RFRKICDEIG AYLMVDMAHY AGLIAAGVYP SPVGIADFIT STTHKTLRGP RGGLILAKAE 250 260 270 280 290 300 YAAVLDKTIF PVYQGGPLMH VIAAKAVAFN EALGDEFKHY QQRVINNART MADVLTRRGL 310 320 330 340 350 360 RVVSGGTDCH MFLLDLRAMN ITGKDAEALL ESAHITLNKN AIPDDPQKPA ITSGIRIGTP 370 380 390 400 410 ALTTRGFGEA ECAEVANLIA DLLEQPDNTA RVENIRRRVM HLCECFPVYL LT

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:RESEARCH51.1-RESEARCH51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA74055.1.
RefSeq	YP_348044.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KDV1.
Genome annotation databases
GeneID	3715536.
GenomeReviews	Gene locus Pfl01_2312 in contig CP000094_GR.
KEGG	pfo:Pfl01_2312.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3KDV1.
OMA	NTARVEN.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_2312-MON.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA1_PSEPF

Accession

Primary (citable) accession number: Q3KDV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)