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Q3KDV1 (GLYA1_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 1
Short name=SHMT 1
Short name=Serine methylase 1
EC=2.1.2.1
Gene names
Name:glyA1
Ordered Locus Names:Pfl01_2312
OrganismPseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]
Taxonomic identifier205922 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 1
PRO_0000235004

Regions

Region120 – 1223Substrate binding By similarity

Sites

Binding site301Pyridoxal phosphate By similarity
Binding site501Pyridoxal phosphate By similarity
Binding site521Substrate By similarity
Binding site591Substrate binding By similarity
Binding site601Pyridoxal phosphate By similarity
Binding site941Pyridoxal phosphate By similarity
Binding site1161Substrate By similarity
Binding site1711Pyridoxal phosphate By similarity
Binding site1991Pyridoxal phosphate By similarity
Binding site2241Pyridoxal phosphate By similarity
Binding site2311Pyridoxal phosphate By similarity
Binding site2561Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3561Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2251N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3KDV1 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: BAB46C25DD93B93C

FASTA41245,462
        10         20         30         40         50         60 
MSLQNFDPAI ARLIDRERNR QETHLELIAS ENYVSEEVLQ AQGSLLTNKY AEGYPGRRYY 

        70         80         90        100        110        120 
GGCKVVDEIE NLAIERARKL FGCEYVNVQP HSGSQANQAV FLAVLEPGDR ILGMSLAHGG 

       130        140        150        160        170        180 
HLTHGASVNF SGKFFQAFTY GLEKDSETLD YDQMEDLARE HRPKMIIAGA SAYSRVIDFP 

       190        200        210        220        230        240 
RFRKICDEIG AYLMVDMAHY AGLIAAGVYP SPVGIADFIT STTHKTLRGP RGGLILAKAE 

       250        260        270        280        290        300 
YAAVLDKTIF PVYQGGPLMH VIAAKAVAFN EALGDEFKHY QQRVINNART MADVLTRRGL 

       310        320        330        340        350        360 
RVVSGGTDCH MFLLDLRAMN ITGKDAEALL ESAHITLNKN AIPDDPQKPA ITSGIRIGTP 

       370        380        390        400        410 
ALTTRGFGEA ECAEVANLIA DLLEQPDNTA RVENIRRRVM HLCECFPVYL LT

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000094 Genomic DNA. Translation: ABA74055.1.
RefSeqYP_348044.1. NC_007492.2.

3D structure databases

ProteinModelPortalQ3KDV1.
SMRQ3KDV1. Positions 7-397.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ3KDV1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3715536.
GenomeReviewsGene locus Pfl01_2312 in contig CP000094_GR.
KEGGpfo:Pfl01_2312.
PATRIC19886785. VBIPseFlu44242_2346.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0112.
HOGENOMHBG301263.
OMAPRDWDYG.
PhylomeDBQ3KDV1.
ProtClustDBPRK00011.

Enzyme and pathway databases

BioCycPFLU205922:PFL_2312-MONOMER.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA1_PSEPF
AccessionPrimary (citable) accession number: Q3KDV1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 8, 2005
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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