Reviewed, UniProtKB/Swiss-Prot Q3KCB2 (ARLY1_PSEPF)

Names and origin

Protein names

Recommended name:
    Argininosuccinate lyase 1
      Short name=ASAL 1
    EC=4.3.2.1
Alternative name(s):
    Arginosuccinase 1

Gene names

Name:	argH1
Ordered Locus Names:	Pfl01_2852

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. HAMAP MF_00006
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3. HAMAP MF_00006
Subcellular location	Cytoplasm Probable HAMAP MF_00006.
Sequence similarities	Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process via ornithine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	argininosuccinate lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Argininosuccinate lyase 1 HAMAP MF_00006		PRO_0000240752

Sequences

	Sequence		Length	Mass (Da)
	Q3KCB2-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 076B831E8988AA60	FASTA	475	51,912
	10 20 30 40 50 60 MSQTTDRLWG ARFKSGPSAA LAALSRCPER YFRLTPYDLA GSKAHAGELQ RAGLLDEQET 70 80 90 100 110 120 RTMIEALDGI GADFAAGRIA PTLDDEDVHT FIERLLTERL GALGGKLRAG RSRNDQTAND 130 140 150 160 170 180 LRLFLRDHVR TLAVEVLALQ TALVDQAEQH VESICPGFTH LQQAQPIVFA HHLLAHAQSM 190 200 210 220 230 240 LRDVQRLMDW DARTSLSPLG AAAMAGSAIA RQPQQSAKEM GYAGVCENSI DAVASRDHVA 250 260 270 280 290 300 EFLFIASMLG INISRLAEEF CLWSSRQFRW VDLDDAYATG SSIMPQKKNP DIAELARGKA 310 320 330 340 350 360 GRLIGNLTGL LSTLKSLPLS YNRDLSEDKN GVLDSVDTLL LVLPAMAGMV ATMTVNVEEL 370 380 390 400 410 420 RRQAPLGFTL ATEVADWLAV RGVPFKEAHE ITGALVQACE KHDLELWEAS PALLAEIDPR 430 440 450 460 470 LTADVRDSLT LEAAIAARSG WGGTAPQQVR EQIGRLKTAL AAQQQWTENY QGFRL

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA74593.1.
RefSeq	YP_348583.1.
3D structure databases
SMR	Q3KCB2. Positions 4-400, 6-453, 32-462.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KCB2.
Genome annotation databases
GeneID	3712172.
GenomeReviews	Gene locus Pfl01_2852 in contig CP000094_GR.
KEGG	pfo:Pfl01_2852.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0165.
HOGENOM	HBG539632.
OMA	GALWGGR.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_2852-MONOMER.
Family and domain databases
HAMAP	MF_00006. Arg_succ_lyase. [Tree]
InterPro	IPR009049. Argininosuccinate_lyase. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. [Graphical view]
PANTHER	PTHR11444:SF3. argH. 1 hit.
Pfam	PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00838. argH. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLY1_PSEPF

Accession

Primary (citable) accession number: Q3KCB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)