Q3KC83 (NUDC_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH pyrophosphatase
EC=3.6.1.22

Gene names

Name:	nudC
Ordered Locus Names:	Pfl01_2881

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	NAD+ + H2O = AMP + NMN. HAMAP MF_00297
Cofactor	Divalent metal cations. Magnesium or manganese By similarity. Binds 1 zinc ion per subunit By similarity. HAMAP MF_00297
Subunit structure	Homodimer By similarity. HAMAP MF_00297
Sequence similarities	Belongs to the Nudix hydrolase family. NudC subfamily. Contains 1 nudix hydrolase domain.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	NAD+ diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	NADH pyrophosphatase HAMAP MF_00297		PRO_1000071962
Regions
	Domain	139 – 262	124	Nudix hydrolase
	Motif	173 – 194	22	Nudix box HAMAP MF_00297
Sites
	Metal binding	112	1	Zinc By similarity
	Metal binding	115	1	Zinc By similarity
	Metal binding	130	1	Zinc By similarity
	Metal binding	133	1	Zinc By similarity
	Metal binding	188	1	Divalent metal cation By similarity
	Metal binding	192	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3KC83 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 5A4DDCA0FBE27236	FASTA	276	31,000
	10 20 30 40 50 60 MTSRWTTAVL DTDQPGGWAV ARSPEGFLFD DNGVLFPREW LKRQDLSILA EHGIGHLDGE 70 80 90 100 110 120 PVYLLELHST SEVPGCNWKG LRAFMLDGDH TIYKVLGYAA QIGTWAREHR FCGNCGQAMT 130 140 150 160 170 180 QVPRERAMYC QPCDLRSYPR ISPSMIVLVT RGDEVLLARS PRFVTGVYST LAGFAEPGES 190 200 210 220 230 240 AEDCLIREVR EEVQIEVKNI QYMGSQCWPF PHSMMLGFHA EYAGGEIVCQ EDEIEDAQWF 250 260 270 NVHDLPPLPA SKSIARYLID VYVARRLGHA EPVLPG

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf0-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA74622.1.
RefSeq	YP_348612.1. NC_007492.2.
3D structure databases
HSSP	HSSP built from PDB template 1VK6 based on UniProtKB P32664.
ProteinModelPortal	Q3KC83.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KC83.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3712429.
GenomeReviews	Gene locus Pfl01_2881 in contig CP000094_GR.
KEGG	pfo:Pfl01_2881.
PATRIC	19887927. VBIPseFlu44242_2916.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2816.
HOGENOM	HBG736436.
OMA	YPRISPS.
ProtClustDB	PRK00241.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_2881-MONOMER.
Family and domain databases
HAMAP	MF_00297. Nudix_NudC. [Tree]
InterPro	IPR015375. NADH_PPase-like_N. IPR022925. NADH_pyroPase_NudC. IPR000086. NUDIX_hydrolase_dom. IPR015797. NUDIX_hydrolase_dom-like. IPR015376. Znr_NADH_PPase. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
KO	K03426.
Pfam	PF00293. NUDIX. 1 hit. PF09296. NUDIX-like. 1 hit. PF09297. zf-NADH-PPase. 1 hit. [Graphical view]
SUPFAM	SSF55811. NUDIX_hydrolase. 2 hits.
PROSITE	PS51462. NUDIX. 1 hit. PS00893. NUDIX_BOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUDC_PSEPF

Accession

Primary (citable) accession number: Q3KC83

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program