Reviewed, UniProtKB/Swiss-Prot Q3KBG7 (DNAE2_PSEPF)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Pfl01_3149

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	1025 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1025	1025	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000070594

Sequences

	Sequence		Length	Mass (Da)
	Q3KBG7-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: B1C2F0E4DB44A93F	FASTA	1,025	115,238
	10 20 30 40 50 60 MNQGYAELHC LSNFSFQRGA SSALELFQRA KKHGYQALAI TDECTLAGIV RAWQAAKSVE 70 80 90 100 110 120 LPLIIGSEIR IENGPKLVLL VENIEGYQAL CGLITQARRR TQKGQYQILR EDFSEPLPGL 130 140 150 160 170 180 LVLWVPEAVD EVEEGRWLKQ TFGERLWLAV QLHRGQNDQQ RLAALLSLAD ELQIPAVASG 190 200 210 220 230 240 DVHMHARGRR ALQDTMTAIR HHVPVAEAGL RLHPNGERHL RSLDVLRELY PQTLLDESLK 250 260 270 280 290 300 LARRCTFDLG ELRYQYPKEL VPEEHSASSW LRHLTEQGIA WRWPKGAQPK VLKQIDDELE 310 320 330 340 350 360 LIAELGYESY FLTVHDVVRF AREQKILCQG RGSAANSAVC FALGITEIDP DRTTLLFERF 370 380 390 400 410 420 MSRERNEPPD IDVDFEHERR EEVLQYVFRR YGRRRAALTA VVSTYHASGA IRDVAKALGL 430 440 450 460 470 480 PPDQINALAD CCGHWSDETP PVERLREGGF DPESPLLHRV LSLTGQLIGF PRHLSQHPGG 490 500 510 520 530 540 FVISEQPLDT LVPVENAAMA DRTIIQWDKD DLDAVGLLKV DILALGMLSA IRRCFDLLRR 550 560 570 580 590 600 HRHQDLSLAT IPPEDRPTYD MISRADTIGV FQIESRAQMS MLPRLRPQTF YDLVIEVAIV 610 620 630 640 650 660 RPGPIQGGMV HPYLRRRNKE EEETYPSPEL EVVLKRTLGV PLFQEQVMQI AIVAADYSPG 670 680 690 700 710 720 EADQLRRSMA AWKRHGGLEP HKERLAAGMK KNGYSPEFAA QIFEQIKGFG SYGFPESHAA 730 740 750 760 770 780 SFALLTYASC WLKCHEPAAF ACALINSWPM GFYSPDQILQ DARRHHLQIR PVDVRASDWD 790 800 810 820 830 840 CSLEPIAGEQ PAIRMGLRMI KGFREEDARS IEKARARGAF ADVADLGERA GLDSRAQALL 850 860 870 880 890 900 ADAGALRGLA GHRHRARWEV AGVQKQLGLF AGLPSQEEPD VLLPTPSVSE DLFTDYATLG 910 920 930 940 950 960 TTLGPHPLTL LRNELRARRC RSSRDLLEVE HGRPVSVAGL VTGRQRPGTA SGVTFVTLED 970 980 990 1000 1010 1020 EFGNVNVVVW RDLAERQRQV LVGSQLLKVD GRWEREGEVR HLIAGRLSDL TPLLNGIRVQ SRDFH

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:RESEARCH51.1-RESEARCH51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA74887.1.
RefSeq	YP_348878.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KBG7.
Genome annotation databases
GeneID	3712874.
GenomeReviews	Gene locus Pfl01_3149 in contig CP000094_GR.
KEGG	pfo:Pfl01_3149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3KBG7.
OMA	MGFYSPD.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_3149-MON.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_PSEPF

Accession

Primary (citable) accession number: Q3KBG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)