Q3KA79 (LFTR_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucyl/phenylalanyl-tRNA--protein transferase
EC=2.3.2.6

Alternative name(s):
L/F-transferase
Leucyltransferase
Phenyalanyltransferase

Gene names

Name:	aat
Ordered Locus Names:	Pfl01_3587

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas fluorescens

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine By similarity. HAMAP-Rule MF_00688
Catalytic activity	L-leucyl-tRNA + protein = tRNA + L-leucyl-protein. HAMAP-Rule MF_00688 L-phenylalanyl-tRNA + protein = tRNA + L-phenylalanyl-protein. HAMAP-Rule MF_00688
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L/F-transferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	leucyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Leucyl/phenylalanyl-tRNA--protein transferase HAMAP-Rule MF_00688		PRO_0000258078

Sequences

	Sequence		Length	Mass (Da)
	Q3KA79 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: EB3B6EE63FDA77F2	FASTA	226	25,735
	10 20 30 40 50 60 MLTWLQRNSL TFPPLEKAMR DPNGLLAAGG DLSADRLIQA YRHGCFPWFS EGQPILWWSP 70 80 90 100 110 120 DPRTVLFPDE LHVSRSLGKL MRKQRYRVTF DQDFDAVIRA CAAPREYADG TWITEAMQDA 130 140 150 160 170 180 YIELHRRGFA HSVEVWDEGE LVGGLYGLAM GQLFFGESMF SRADNASKYG FATLVQHLRE 190 200 210 220 AGFVLIDCQM PTDHLHSLGA RAISRQTFAD YLARHLDQPN HATWVC

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf0-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA75325.1.
RefSeq	YP_349316.1. NC_007492.2.
3D structure databases
HSSP	HSSP built from PDB template 2CXA based on UniProtKB P0A8P1.
ProteinModelPortal	Q3KA79.
SMR	Q3KA79. Positions 2-220.
ModBase	Search...
Protein-protein interaction databases
STRING	205922.Pfl01_3587.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABA75325; ABA75325; Pfl01_3587.
GeneID	3714975.
KEGG	pfo:Pfl01_3587.
PATRIC	19889265. VBIPseFlu44242_3582.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2360.
HOGENOM	HOG000102325.
KO	K00684.
OMA	MFYGESM.
ProtClustDB	PRK00301.
Enzyme and pathway databases
BioCyc	PFLU205922:GJBD-3634-MONOMER.
Family and domain databases
HAMAP	MF_00688. Leu_Phe_trans.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR004616. Leu/Phe-tRNA_Trfase. [Graphical view]
Pfam	PF03588. Leu_Phe_trans. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR00667. aat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LFTR_PSEPF

Accession

Primary (citable) accession number: Q3KA79

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program