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Protein

UDP-2,3-diacylglucosamine hydrolase

Gene

lpxH

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP by catalyzing the attack of water at the alpha-P atom. Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + H2O = 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate + UMP.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 Mn2+ ions per subunit in a binuclear metal center.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Manganese 1UniRule annotation1
Metal bindingi9Manganese 1; via tele nitrogenUniRule annotation1
Metal bindingi40Manganese 1UniRule annotation1
Metal bindingi40Manganese 2UniRule annotation1
Metal bindingi78Manganese 2UniRule annotation1
Metal bindingi113Manganese 2; via tele nitrogenUniRule annotation1
Binding sitei121SubstrateUniRule annotation1
Binding sitei159SubstrateUniRule annotation1
Binding sitei163SubstrateUniRule annotation1
Binding sitei166SubstrateUniRule annotation1
Metal bindingi194Manganese 2; via pros nitrogenUniRule annotation1
Binding sitei194Substrate; via tele nitrogenUniRule annotation1
Metal bindingi196Manganese 1; via tele nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandManganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00359; UER00480.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-2,3-diacylglucosamine hydrolaseUniRule annotation (EC:3.6.1.54UniRule annotation)
Alternative name(s):
UDP-2,3-diacylglucosamine diphosphataseUniRule annotation
Gene namesi
Name:lpxHUniRule annotation
Ordered Locus Names:Pfl01_3641
OrganismiPseudomonas fluorescens (strain Pf0-1)
Taxonomic identifieri205922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002704 Componenti: Chromosome

Subcellular locationi

Q3KA25:
  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000250741 – 248UDP-2,3-diacylglucosamine hydrolaseAdd BLAST248

Proteomic databases

PRIDEiQ3KA25.

Interactioni

Protein-protein interaction databases

STRINGi205922.Pfl01_3641.

Structurei

3D structure databases

ProteinModelPortaliQ3KA25.
SMRiQ3KA25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 79Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the LpxH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F10. Bacteria.
COG2908. LUCA.
HOGENOMiHOG000261930.
KOiK03269.
OMAiFDFWFEY.
OrthoDBiPOG091H064W.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
HAMAPiMF_00575. LpxH. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR010138. UDP-diacylglucosamine_Hdrlase.
PANTHERiPTHR34990:SF1. PTHR34990:SF1. 1 hit.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR01854. lipid_A_lpxH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3KA25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILLISDLHL EEERPDITRA FLDLLAGRAR SASALYILGD FFEAWIGDDA
60 70 80 90 100
MTPFQRSICQ ALRELSDSGT AIFLMHGNRD FMLGKAFCKQ AGCTLLKDPS
110 120 130 140 150
VVQFYGEPVL LMHGDSLCTR DESYMKLRRY LRNPLSLFIL RNLPLRTRHK
160 170 180 190 200
LARKLRSESR AQTRMKANDI VDVTPEEIPR IMQEYGVKTL VHGHTHRPAI
210 220 230 240
HKLQIGDQAA KRIVLGDWDR QGWALQVDEN GFALAPFDFA PPLALPHG
Length:248
Mass (Da):28,165
Last modified:November 8, 2005 - v1
Checksum:i4D6582C1B2467CA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA. Translation: ABA75379.1.
RefSeqiWP_011334986.1. NC_007492.2.

Genome annotation databases

EnsemblBacteriaiABA75379; ABA75379; Pfl01_3641.
KEGGipfo:Pfl01_3641.

Similar proteinsi

Entry informationi

Entry nameiLPXH_PSEPF
AccessioniPrimary (citable) accession number: Q3KA25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: October 25, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families