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Protein

UDP-2,3-diacylglucosamine hydrolase

Gene

lpxH

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + H2O = 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + UMP.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciPFLU205922:GJBD-3688-MONOMER.
UniPathwayiUPA00359; UER00480.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-2,3-diacylglucosamine hydrolaseUniRule annotation (EC:3.6.1.54UniRule annotation)
Alternative name(s):
UDP-2,3-diacylglucosamine diphosphataseUniRule annotation
Gene namesi
Name:lpxHUniRule annotation
Ordered Locus Names:Pfl01_3641
OrganismiPseudomonas fluorescens (strain Pf0-1)
Taxonomic identifieri205922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002704 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248UDP-2,3-diacylglucosamine hydrolasePRO_1000025074Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi205922.Pfl01_3641.

Structurei

3D structure databases

ProteinModelPortaliQ3KA25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F10. Bacteria.
COG2908. LUCA.
HOGENOMiHOG000261930.
KOiK03269.
OMAiFMHGNRD.
OrthoDBiEOG6FNHR2.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
HAMAPiMF_00575. LpxH.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR010138. UDP-diacylglucosamine_Hdrlase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR01854. lipid_A_lpxH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3KA25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILLISDLHL EEERPDITRA FLDLLAGRAR SASALYILGD FFEAWIGDDA
60 70 80 90 100
MTPFQRSICQ ALRELSDSGT AIFLMHGNRD FMLGKAFCKQ AGCTLLKDPS
110 120 130 140 150
VVQFYGEPVL LMHGDSLCTR DESYMKLRRY LRNPLSLFIL RNLPLRTRHK
160 170 180 190 200
LARKLRSESR AQTRMKANDI VDVTPEEIPR IMQEYGVKTL VHGHTHRPAI
210 220 230 240
HKLQIGDQAA KRIVLGDWDR QGWALQVDEN GFALAPFDFA PPLALPHG
Length:248
Mass (Da):28,165
Last modified:November 8, 2005 - v1
Checksum:i4D6582C1B2467CA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA. Translation: ABA75379.1.
RefSeqiWP_011334986.1. NC_007492.2.

Genome annotation databases

EnsemblBacteriaiABA75379; ABA75379; Pfl01_3641.
KEGGipfo:Pfl01_3641.
PATRICi19889375. VBIPseFlu44242_3637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA. Translation: ABA75379.1.
RefSeqiWP_011334986.1. NC_007492.2.

3D structure databases

ProteinModelPortaliQ3KA25.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi205922.Pfl01_3641.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA75379; ABA75379; Pfl01_3641.
KEGGipfo:Pfl01_3641.
PATRICi19889375. VBIPseFlu44242_3637.

Phylogenomic databases

eggNOGiENOG4105F10. Bacteria.
COG2908. LUCA.
HOGENOMiHOG000261930.
KOiK03269.
OMAiFMHGNRD.
OrthoDBiEOG6FNHR2.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00480.
BioCyciPFLU205922:GJBD-3688-MONOMER.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
HAMAPiMF_00575. LpxH.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR010138. UDP-diacylglucosamine_Hdrlase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR01854. lipid_A_lpxH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf0-1.

Entry informationi

Entry nameiLPXH_PSEPF
AccessioniPrimary (citable) accession number: Q3KA25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: December 9, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.