Reviewed, UniProtKB/Swiss-Prot Q3K9F5 (STHA_PSEPF)

Names and origin

Protein names

Recommended name:
    Soluble pyridine nucleotide transhydrogenase
      Short name=STH
    EC=1.6.1.1
Alternative name(s):
    NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Ordered Locus Names:	Pfl01_3862

Organism

Pseudomonas fluorescens (strain PfO-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity.
Catalytic activity	NADPH + NAD(+) = NADP(+) + NADH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Soluble pyridine nucleotide transhydrogenase		PRO_0000260239
Regions
	Nucleotide binding	35 – 44	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3K9F5-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: BB34A1B3B5826D16	FASTA	464	50,891
	10 20 30 40 50 60 MAVYNYDVVV LGSGPAGEGA AMNAAKAGRK VAMVDSRRQV GGNCTHLGTI PSKALRHSVR 70 80 90 100 110 120 QIMQFNTNPM FRAIGEPRWF SFPDVLKSAE KVISKQVASR TGYYARNRVD VFFGTGSFAD 130 140 150 160 170 180 EQTIEVVCAN GVVEKLVAKH IIIATGSRPY RPADIDFHHP RIYDSDTILS LGHTPRKLIV 190 200 210 220 230 240 YGAGVIGCEY ASIFSGLGVL VELVDNRGQL LSFLDSEISQ ALSYHFSNNN ITVRHNEEYD 250 260 270 280 290 300 RVEGVDNGVI LHLKSGKKIK ADALLWCNGR TGNTDQLGLE NIGVKVNSRG QIEVDENYRT 310 320 330 340 350 360 CVQNIYGAGD VIGWPSLASA AHDQGRSAAG SIVDNGSWRF VNDVPTGIYT IPEISSIGKN 370 380 390 400 410 420 EQELTQAKVP YEVGKAFFKS MARAQIAGEP QGMLKILFHR ETLEVLGVHC FGYQASEIVH 430 440 450 460 IGQAIMNQPG ELNTLKYFVN TTFNYPTMAE AYRVAAYDGL NRLF

References

[1]

"Complete sequence of Pseudomonas fluorescens PfO-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyripides N., Anderson I., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA75599.1.
RefSeq	YP_349590.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3712292.
GenomeReviews	Gene locus Pfl01_3862 in contig CP000094_GR.
KEGG	pfo:PflO1_3862.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3K9F5.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_3862-MON.
Family and domain databases
HAMAP	MF_00247. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

STHA_PSEPF

Accession

Primary (citable) accession number: Q3K9F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 8, 2005
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)