ID   FADB_PSEPF              Reviewed;         715 AA.
AC   Q3K9D8;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   16-JUN-2009, entry version 35.
DE   RecName: Full=Fatty acid oxidation complex subunit alpha;
DE   Includes:
DE     RecName: Full=Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase;
DE              EC=4.2.1.17;
DE              EC=5.3.3.8;
DE              EC=5.1.2.3;
DE   Includes:
DE     RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
DE              EC=1.1.1.35;
GN   Name=fadB; OrderedLocusNames=Pfl01_3879;
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J.,
RA   Larimer F., Land M., Kyripides N., Anderson I., Richardson P.;
RT   "Complete sequence of Pseudomonas fluorescens PfO-1.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of an hydroxyacyl-CoA by
CC       addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA
CC       epimerase and 3-hydroxyacyl-CoA dehydrogenase activities (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA
CC       + NADH.
CC   -!- CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-
CC       CoA + H(2)O.
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxybutanoyl-CoA = (R)-3-
CC       hydroxybutanoyl-CoA.
CC   -!- CATALYTIC ACTIVITY: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-
CC       CoA.
CC   -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC   -!- SUBUNIT: Heterotetramer of two alpha chains (fadB) and two beta
CC       chains (fadA) (By similarity).
CC   -!- SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA
CC       hydratase/isomerase family.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the 3-
CC       hydroxyacyl-CoA dehydrogenase family.
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DR   EMBL; CP000094; ABA75616.1; -; Genomic_DNA.
DR   RefSeq; YP_349607.1; -.
DR   SMR; Q3K9D8; 1-715.
DR   GeneID; 3712525; -.
DR   GenomeReviews; CP000094_GR; Pfl01_3879.
DR   KEGG; pfo:Pfl01_3879; -.
DR   HOGENOM; Q3K9D8; -.
DR   OMA; Q3K9D8; ANNGSYY.
DR   BioCyc; PFLU205922:PFL_3879-MON; -.
DR   GO; GO:0016507; C:fatty acid beta-oxidation multienzyme complex; IEA:InterPro.
DR   GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity; IEA:HAMAP.
DR   GO; GO:0050662; F:coenzyme binding; IEA:InterPro.
DR   GO; GO:0004165; F:dodecenoyl-CoA delta-isomerase activity; IEA:HAMAP.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:HAMAP.
DR   GO; GO:0009062; P:fatty acid catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01621; -; 1.
DR   InterPro; IPR006180; 3-OHacyl-CoA_DH_CS.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR001753; Crotonase_core.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR012799; FadB.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   Pfam; PF00378; ECH; 1.
DR   TIGRFAMs; TIGR02437; FadB; 1.
DR   PROSITE; PS00067; 3HCDH; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Fatty acid metabolism; Isomerase;
KW   Lipid degradation; Lipid metabolism; Lyase; Multifunctional enzyme;
KW   NAD; Oxidoreductase.
FT   CHAIN         1    715       Fatty acid oxidation complex subunit
FT                                alpha.
FT                                /FTId=PRO_0000255843.
FT   REGION        1    190       Enoyl-CoA hydratase/isomerase (By
FT                                similarity).
FT   REGION      312    715       3-hydroxyacyl-CoA dehydrogenase (By
FT                                similarity).
SQ   SEQUENCE   715 AA;  77389 MW;  5D10ACED70DE4C71 CRC64;
     MIYEGKAITV KALESGIVEL KFDLKGESVN KFNRLTLNEL RQAVDTIKAD ASIKGVIVSS
     GKDVFIVGAD ITEFVDNFKL PDAELVAGNL EANKIFSDFE DLNVPTVAAI NGIALGGGLE
     MCLAADYRVM STKAKIGLPE VKLGIYPGFG GTVRLPRLIG ADNAIEWIAA GKENRPEDAL
     KVGAVDAVVA PEKLQDAALE LIKRAVSGEF DYKAKRQPKL EKLRLNAIEQ MMAFETAKGF
     VAGQAGPNYP APVEAIKTIQ KAANFGRDKA LEVEAAGFVK LAKTSAAQSL IGLFLNDQEL
     KKKAKAYDEI AKDVKQAAVL GAGIMGGGIA YQSASKGTPI LMKDINEHGI EQGLAEAAKL
     LVGRVDKGRM TAAKMAEVLN GIRPTLSYGD FGHVDLVVEA VVENPKVKQA VLAEVEDKVK
     EDTILASNTS TISISLLAKA LKRPENFVGM HFFNPVHMMP LVEVIRGEKS SELAIATTVA
     YAKKMGKNPI VVNDCPGFLV NRVLFPYFGG FAKLVSAGVD FVRIDKIMEK FGWPMGPAYL
     MDVVGIDTGH HGRDVMAEGF PDRMKDDRRS AIDVLYEAKR LGQKNGKGFY AYEADKKGKQ
     KKVADPSVLE VLKPIVYEQR EVTDEDIINW MMIPLCLETV RCLEDGIVET AAEADMGLVY
     GIGFPPFRGG ALRYIDSIGV AEFVALADQY ADLGALYHPT AKLREMAKNG QSFFG
//