Reviewed, UniProtKB/Swiss-Prot Q3K932 (TPMT_PSEPF)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	Pfl01_3985

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000047214
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3K932-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: BF10193406CA7382	FASTA	217	24,762
	10 20 30 40 50 60 MQPEFWHKKW ESNQIGFHQL EVNPYLQRHW PDLAIPVQAR VLVPLCGKSL DLLWLAGRGH 70 80 90 100 110 120 QVLGVELSEK AVEDFFHEQQ LQPQVSEQGD FKVYRADAVE LWCGDFFSLT MADVAGCTAL 130 140 150 160 170 180 YDRAAVIALP PAMRERYAAH LQSILPACRG LLVTLDYDQS QMPGPPFSVD DAEVQRLLGS 190 200 210 VWRVEMLEQQ DVLGDSWKFV QAGVTRLEER VYRIRGV

References

[1]

"Complete sequence of Pseudomonas fluorescens PfO-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyripides N., Anderson I., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA75722.1.
RefSeq	YP_349713.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3714011.
GenomeReviews	Gene locus Pfl01_3985 in contig CP000094_GR.
KEGG	pfo:Pfl01_3985.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3K932.
OMA	Q3K932. PPFAVSP.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_3985-MON.
Family and domain databases
HAMAP	MF_00812. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_PSEPF

Accession

Primary (citable) accession number: Q3K932

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)