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Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrF

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131SubstrateUniRule annotation
Binding sitei35 – 351SubstrateUniRule annotation
Active sitei64 – 641Proton donorUniRule annotation
Binding sitei122 – 1221SubstrateUniRule annotation
Binding sitei182 – 1821SubstrateUniRule annotation
Binding sitei191 – 1911SubstrateUniRule annotation
Binding sitei211 – 2111Substrate; via amide nitrogenUniRule annotation
Binding sitei212 – 2121SubstrateUniRule annotation

GO - Molecular functioni

  1. orotidine-5'-phosphate decarboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BioCyciPFLU205922:GJBD-4256-MONOMER.
UniPathwayiUPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylaseUniRule annotation (EC:4.1.1.23UniRule annotation)
Alternative name(s):
OMP decarboxylaseUniRule annotation
Short name:
OMPDCaseUniRule annotation
Short name:
OMPdecaseUniRule annotation
Gene namesi
Name:pyrFUniRule annotation
Ordered Locus Names:Pfl01_4196
OrganismiPseudomonas fluorescens (strain Pf0-1)
Taxonomic identifieri205922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002704 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Orotidine 5'-phosphate decarboxylasePRO_0000241890Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi205922.Pfl01_4196.

Structurei

3D structure databases

ProteinModelPortaliQ3K8H1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 7110Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the OMP decarboxylase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0284.
HOGENOMiHOG000226070.
KOiK01591.
OrthoDBiEOG6N6815.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01200_B. OMPdecase_type1_B.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3K8H1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSACQTPIIV ALDFPTRDAA LKLADQLDPK LCRVKVGKEL FTSCAAEIVG
60 70 80 90 100
TLRDKGFEVF LDLKFHDIPN TTAMAVKAAA EMGVWMVNVH CSGGLRMMAA
110 120 130 140 150
CREELDKRSG PQPLLIGVTV LTSMEREDLA GIGLDIEPQE QVLRLAALAE
160 170 180 190 200
KAGMDGLVCS ALEATALKTA HPSLQLVTPG IRPAGSAQDD QRRILTPRQA
210 220 230
LDAGSDYLVI GRPISQAADP AKALASVVAE LA
Length:232
Mass (Da):24,628
Last modified:June 26, 2006 - v2
Checksum:iAAC4A383BF923828
GO

Sequence cautioni

The sequence ABA75933.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA. Translation: ABA75933.1. Different initiation.
RefSeqiYP_349924.1. NC_007492.2.

Genome annotation databases

EnsemblBacteriaiABA75933; ABA75933; Pfl01_4196.
KEGGipfo:Pfl01_4196.
PATRICi19890524. VBIPseFlu44242_4195.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA. Translation: ABA75933.1. Different initiation.
RefSeqiYP_349924.1. NC_007492.2.

3D structure databases

ProteinModelPortaliQ3K8H1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi205922.Pfl01_4196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA75933; ABA75933; Pfl01_4196.
KEGGipfo:Pfl01_4196.
PATRICi19890524. VBIPseFlu44242_4195.

Phylogenomic databases

eggNOGiCOG0284.
HOGENOMiHOG000226070.
KOiK01591.
OrthoDBiEOG6N6815.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00120.
BioCyciPFLU205922:GJBD-4256-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01200_B. OMPdecase_type1_B.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf0-1.

Entry informationi

Entry nameiPYRF_PSEPF
AccessioniPrimary (citable) accession number: Q3K8H1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2006
Last sequence update: June 26, 2006
Last modified: March 31, 2015
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.