Reviewed, UniProtKB/Swiss-Prot Q3K885 (ASTD_PSEPF)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	Pfl01_4282

Organism

Pseudomonas fluorescens (strain PfO-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD(+) + H(2)O = N-succinyl-L-glutamate + NADH.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	N-succinylglutamate 5-semialdehyde dehydrogenase		PRO_0000262415
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3K885-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 47B5200E919D5862	FASTA	488	51,250
	10 20 30 40 50 60 MNSLYIAGEW LAGQGEAFQS LNPVTQQVLW SGEGATAAQV ESAVQAARQA FPGWARRTLE 70 80 90 100 110 120 DRISVLEAFA AALKNHADEL ARTIGEETGK PLWEAATEVT SMVNKIAISV QSYRERTGEK 130 140 150 160 170 180 SGPLGDATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNSVLFKP SELTPKVAEL 190 200 210 220 230 240 TVKCWIEAGL PAGVLNLLQG ARETGIALAA NPGIDGLFFT GSSRTGNHLH QQFAGRPDKI 250 260 270 280 290 300 LALEMGGNNP LVVDQVADID AAVYTIIQSA FISAGQRCTC ARRLLVPEGA WGDSLLKRLV 310 320 330 340 350 360 EVSSTIEVGA FDQQPAPFMG SVVSLGAAKA LMDAQAHLLA NGAVSLLAMT QPQAQSALLT 370 380 390 400 410 420 PGIVDVTAVA DRSDEELFGP LLQVIRYADF AAAIAEANDT AFGLAAGLLS DSEERYQQFW 430 440 450 460 470 480 LESRAGIVNW NKQLTGAASS APFGGVGASG NHRASAYYAA DYCAYPVASL ETPSLVLPAA LTPGVKMA

References

[1]

"Complete sequence of Pseudomonas fluorescens PfO-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyripides N., Anderson I., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA76019.1.
RefSeq	YP_350010.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3712894.
GenomeReviews	Gene locus Pfl01_4282 in contig CP000094_GR.
KEGG	pfo:PflO1_4282.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3K885.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_4282-MON.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_PSEPF

Accession

Primary (citable) accession number: Q3K885

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 8, 2005
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)