Reviewed, UniProtKB/Swiss-Prot Q3K7X5 (GCSP1_PSEPF)

Names and origin

Protein names

Recommended name:
    Glycine dehydrogenase [decarboxylating] 1
    EC=1.4.4.2
Alternative name(s):
    Glycine decarboxylase 1
    Glycine cleavage system P-protein 1

Gene names

Name:	gcvP1
Ordered Locus Names:	Pfl01_4392

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	950 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity.
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP MF_00711
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the gcvP family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 950	950	Glycine dehydrogenase [decarboxylating] 1 HAMAP MF_00711		PRO_0000227118
Amino acid modifications
	Modified residue	704	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3K7X5-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: C03E37A90AFB210C	FASTA	950	102,581
	10 20 30 40 50 60 MTQVNLGTAN EFIARHIGPR AGDEQAMLNS LGFDSLEALS ASVIPESIKG TSVLGLDDGL 70 80 90 100 110 120 SEADALAMIK GIAGKNQLFK TYIGQGYYNC HTPSPILRNL LENPAWYTAY TPYQPEISQG 130 140 150 160 170 180 RLEALLNFQT LISDLTGLPI ANASLLDEAT AAAEAMTFCK RLSKNKGSHQ FFASIHSHTQ 190 200 210 220 230 240 TLDVLRTRAE PLGIDVVVGD ERELTDVTPF FGALLQYPAS NGDVFDYREL TERFHAANAL 250 260 270 280 290 300 VAVAADLLAL TLLTPPGEFG ADVAIGSAQR FGVPLGFGGP HAAYFSTKDA FKRDMPGRLV 310 320 330 340 350 360 GVSVDRFGKP ALRLAMQTRE QHIRREKATS NICTAQVLLA NIASMYAVYH GPKGLTQIAN 370 380 390 400 410 420 RVHHLTAILA KGLSALGVTV EQTSFFDTLT LATGAQTAAL HDKARAQQIN LRVIDAQRLG 430 440 450 460 470 480 LSVDETTTQA DIETLWGLFA DGKTLPDFAA LAAAAQSTIP ASLVRQSPIL SHPVFNRYHS 490 500 510 520 530 540 ETELMRYLRK LADKDLALDR TMIPLGSCTM KLNAASEMIP VTWAEFGALH PFAPAEQSAG 550 560 570 580 590 600 YQQLTDELEA MLCAATGYDS ISLQPNAGSQ GEYAGLLAIR AYHQSRGEDR RDICLIPSSA 610 620 630 640 650 660 HGTNPATANM AGMRVVVTAC DARGNVDIED LRAKAIEHRE HLAALMITYP STHGVFEEGI 670 680 690 700 710 720 REICGIIHDN GGQVYIDGAN MNAMVGLCAP GKFGGDVSHL NLHKTFCIPH GGGGPGVGPI 730 740 750 760 770 780 GVKSHLTPFL PGHGHMERKE GAVCAAPFGS ASILPITWMY IRMMGGAGLK RASQLAILNA 790 800 810 820 830 840 NYISRRLEEH YPVLYTGSNG LVAHECILDL RPLKDSSGIS VDDVAKRLID FGFHAPTMSF 850 860 870 880 890 900 PVAGTLMIEP TESESKEELD RFCDAMIRIR EEIRAVENGT LDKDDNPLKN APHTAAELVG 910 920 930 940 950 EWTHPYSREQ AVYPVASLIE GKYWPPVGRV DNVFGDRNLV CACPSIESYA

References

[1]

"Complete sequence of Pseudomonas fluorescens PfO-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyripides N., Anderson I., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA76129.1.
RefSeq	YP_350120.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3712835.
GenomeReviews	Gene locus Pfl01_4392 in contig CP000094_GR.
KEGG	pfo:Pfl01_4392.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3K7X5.
OMA	Q3K7X5. PETIMIE.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_4392-MON.
Family and domain databases
HAMAP	MF_00711. [Tree]
InterPro	IPR003437. GDC-P. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11773. GDC-P. 1 hit.
Pfam	PF02347. GDC-P. 1 hit. [Graphical view]
TIGRFAMs	TIGR00461. gcvP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCSP1_PSEPF

Accession

Primary (citable) accession number: Q3K7X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)