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Q3K7K0 (ASSY_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Argininosuccinate synthase
EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:Pfl01_4517
OrganismPseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]
Taxonomic identifier205922 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 405405Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000263955

Regions

Nucleotide binding10 – 189ATP By similarity

Sites

Binding site371ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site881Citrulline By similarity
Binding site931Citrulline By similarity
Binding site1181ATP; via amide nitrogen By similarity
Binding site1201Aspartate By similarity
Binding site1241Aspartate By similarity
Binding site1241Citrulline By similarity
Binding site1251Aspartate By similarity
Binding site1281Citrulline By similarity
Binding site1791Citrulline By similarity
Binding site1881Citrulline By similarity
Binding site2641Citrulline By similarity
Binding site2761Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3K7K0 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 5F3D40605DA91B87

FASTA40545,420
        10         20         30         40         50         60 
MADVNKVVLA YSGGLDTSVI LKWLQDTYNC EVVTFTADLG QGEEVEPARA KAQAMGVKEI 

        70         80         90        100        110        120 
YIDDLREEFV RDFVFPMFRA NTVYEGEYLL GTSIARPLIA KRLIEIANET GADAISHGAT 

       130        140        150        160        170        180 
GKGNDQVRFE LGAYALKPGV KVIAPWREWD LLSREKLMDY AEKHAIPIER HGKKKSPYSM 

       190        200        210        220        230        240 
DANLLHISYE GGVLEDTWTE HEEDMWRWTV SPEKAPDTPQ YLELTYRNGD IVALDGVEMT 

       250        260        270        280        290        300 
PATVLATLNR IGGEHGIGRL DIVENRYVGM KSRGCYETPG GTIMLRAHRA IESITLDREV 

       310        320        330        340        350        360 
AHLKDELMPK YASLIYTGYW WSPERLMLQQ MIDASQVNVN GVVRLKLYKG NVIVTGRKSD 

       370        380        390        400 
DSLFDANIAT FEEDGGAYNQ ADAAGFIKLN ALRMRIAANK GRKLF

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000094 Genomic DNA. Translation: ABA76254.1.
RefSeqYP_350245.1. NC_007492.2.

3D structure databases

HSSPHSSP built from PDB template 1VL2 based on UniProtKB Q9X2A1.
ProteinModelPortalQ3K7K0.
SMRQ3K7K0. Positions 6-398.
ModBaseSearch...

Protein-protein interaction databases

STRING205922.Pfl01_4517.

2D gel databases

World-2DPAGE0008:Q3K7K0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA76254; ABA76254; Pfl01_4517.
GeneID3717886.
KEGGpfo:Pfl01_4517.
PATRIC19891192. VBIPseFlu44242_4523.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAIYNGYWW.
ProtClustDBPRK00509.

Enzyme and pathway databases

BioCycPFLU205922:GJBD-4583-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR11587. PTHR11587. 1 hit.
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_PSEPF
AccessionPrimary (citable) accession number: Q3K7K0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 8, 2005
Last modified: May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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