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Q3K746 (DDL_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Pfl01_4671
OrganismPseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]
Taxonomic identifier205922 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030486

Regions

Domain116 – 311196ATP-grasp
Nucleotide binding142 – 19756ATP By similarity

Sites

Metal binding2651Magnesium or manganese 1 By similarity
Metal binding2781Magnesium or manganese 1 By similarity
Metal binding2781Magnesium or manganese 2 By similarity
Metal binding2801Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3K746 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: B86436D8182D1BC3

FASTA32434,373
        10         20         30         40         50         60 
MTAAYANLFS TIAPKDFGRV AVLFGGLSAE REVSLKSGNA VLTALQSAGV DAFGIDVGAD 

        70         80         90        100        110        120 
ILQRLLSEKI DRAFIILHGR GGEDGSMQGL LEVAGIPYTG SGILASALAM DKLRTKQVWH 

       130        140        150        160        170        180 
TLGIPTPRHA VLRSEADCIS AATELGFPLI VKPAHEGSSI GMAKVSSASE LIDAWKAAST 

       190        200        210        220        230        240 
YDSQVLVEQW IHGPEFTIAT LRDQVLPPIA LGTPHTFYDY DAKYIANDTQ YRIPCGLDAA 

       250        260        270        280        290        300 
KEKELMDLTA QACEALGIAG WGRADVMQDA DGKFWFLEVN TAPGMTDHSL VPMAAKAAGL 

       310        320 
DFQQLVLAIL AASVDADGKK EARG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000094 Genomic DNA. Translation: ABA76408.1.
RefSeqYP_350399.1. NC_007492.2.

3D structure databases

ProteinModelPortalQ3K746.
SMRQ3K746. Positions 18-311.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING205922.Pfl01_4671.

2D gel databases

World-2DPAGE0008:Q3K746.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA76408; ABA76408; Pfl01_4671.
GeneID3714571.
KEGGpfo:Pfl01_4671.
PATRIC19891502. VBIPseFlu44242_4677.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAANDTQYR.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycPFLU205922:GJBD-4738-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_PSEPF
AccessionPrimary (citable) accession number: Q3K746
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways