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Q3K6L5

- SYI_PSEPF

UniProt

Q3K6L5 - SYI_PSEPF

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
    Binding sitei611 – 6111ATPUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPFLU205922:GJBD-4931-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Pfl01_4852
    OrganismiPseudomonas fluorescens (strain Pf0-1)
    Taxonomic identifieri205922 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002704: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000022108Add
    BLAST

    Proteomic databases

    PRIDEiQ3K6L5.

    PTM databases

    PhosSiteiP0906691.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi205922.Pfl01_4852.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3K6L5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi608 – 6125"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3K6L5-1 [UniParc]FASTAAdd to Basket

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    MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYGK LREIGKDRPK    50
    FVLHDGPPYA NGTIHIGHAL NKILKDMIIR SKTLSGFDAP YVPGWDCHGL 100
    PIEHKVEVTH GKNLGADKTR ELCRAYATEQ IEGQKTEFIR LGVLGDFANP 150
    YKTMNFKNEA GEIRALAEIV KGGFVFKGLK PVNWCFDCGS ALAEAEVEYE 200
    NKKSSTIDVA FPIADEAKLA AAFGLPSLAK PAAIVIWTTT PWTIPANQAL 250
    NVHPEFTYAL VDIGDKLLVL AEELVESCLT RYGVEGTVLA TAPGSALELI 300
    NFRHPFYDRL SPVYLADYVE LGAGTGVVHS APAYGVDDFV TCKKYGMVND 350
    DILNPVQSNG VYVPSLEFFG GQFIWKANPA IVDKLTEVGA LLHTTVIEHS 400
    YMHCWRHKTP LIYRATAQWF IGMDKEPVSG DTLRVRSLKA IEDTKFVPAW 450
    GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LNKESGELHP RTVELMEEVA 500
    KRVEVEGIEA WFKLDAAELL GDEAPQYDKI SDTLDVWFDS GTTHWHVLRG 550
    SHPMGHETGP RADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRELLTHGF 600
    TVDESGRKMS KSLGNVIAPQ KVNDTLGADI MRLWVASTDY SGEMAVSEQI 650
    LQRSADAYRR IRNTARFLLS NLTGFNPATD LLPAEDMLAL DRWAVDRTLL 700
    LQRELQEHYG EYRFWNVYSK IHNFCVQELG GFYLDIIKDR QYTTGADSKA 750
    RRSCQTALFH ISEALVRWIA PILAFTADEL WQYLPGERNE SVMLNTWYEG 800
    LTELPEGFEL GREYWDRIME VKVAVNKEME IQRAAKAVGG NLQAEVTLFA 850
    EDALTADLAK LSNELRFVLI TSTATVAPFV QAPADAVATE VSGLKLKIVK 900
    SAFPKCARCW HCREDVGVNP EHPEICGRCV DNIDGAGEVR HYA 943
    Length:943
    Mass (Da):105,507
    Last modified:November 8, 2005 - v1
    Checksum:i6B46ACE3C698942C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000094 Genomic DNA. Translation: ABA76589.1.
    RefSeqiYP_350580.1. NC_007492.2.

    Genome annotation databases

    EnsemblBacteriaiABA76589; ABA76589; Pfl01_4852.
    GeneIDi3716852.
    KEGGipfo:Pfl01_4852.
    PATRICi19891898. VBIPseFlu44242_4864.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000094 Genomic DNA. Translation: ABA76589.1 .
    RefSeqi YP_350580.1. NC_007492.2.

    3D structure databases

    ProteinModelPortali Q3K6L5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 205922.Pfl01_4852.

    PTM databases

    PhosSitei P0906691.

    Proteomic databases

    PRIDEi Q3K6L5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA76589 ; ABA76589 ; Pfl01_4852 .
    GeneIDi 3716852.
    KEGGi pfo:Pfl01_4852.
    PATRICi 19891898. VBIPseFlu44242_4864.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PFLU205922:GJBD-4931-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pf0-1.

    Entry informationi

    Entry nameiSYI_PSEPF
    AccessioniPrimary (citable) accession number: Q3K6L5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3