Reviewed, UniProtKB/Swiss-Prot Q3K6J0 (GLYA2_PSEPF)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 2
      Short name=Serine methylase 2
      Short name=SHMT 2
    EC=2.1.2.1

Gene names

Name:	glyA2
Ordered Locus Names:	Pfl01_4877

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase 2 HAMAP MF_00051		PRO_0000235005
Amino acid modifications
	Modified residue	229	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3K6J0-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 8FD3F0F5B235A13C	FASTA	417	44,795
	10 20 30 40 50 60 MFSRDLTIAK YDADLFAAME QEAQRQEEHI ELIASENYTS PAVMEAQGSV LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEY VDVVEQLAID RAKELFGADY ANVQPHAGSQ ANAAVYLALL SAGDTILGMS 130 140 150 160 170 180 LAHGGHLTHG ASVSSSGKLY NAIQYGIDAN GLIDYDEVER LAVEHKPKMI VAGFSAYSQI 190 200 210 220 230 240 LDFPRFRAIA DKVGAYLFVD MAHVAGLVAA GVYPNPVPFA DVVTTTTHKT LRGPRGGLIL 250 260 270 280 290 300 AKANADIEKK LNSAVFPGAQ GGPLEHVIAA KAICFKEALQ PEFKAYQQQV VKNAQAMAGV 310 320 330 340 350 360 FIERGFDVVS GGTENHLFLL SLIKQEISGK DADAALGKAF ITVNKNSVPN DPRSPFVTSG 370 380 390 400 410 LRFGTPAVTT RGFKEAECKE LAGWICDILA DLNNEAVIDA VREKVKAICK KLPVYGA

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA76614.1.
RefSeq	YP_350605.1.
3D structure databases
SMR	Q3K6J0. Positions 1-416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3K6J0.
Genome annotation databases
GeneID	3716236.
GenomeReviews	Gene locus Pfl01_4877 in contig CP000094_GR.
KEGG	pfo:Pfl01_4877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0112.
HOGENOM	HBG301263.
OMA	NINDEAV.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_4877-MONOMER.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_PSEPF

Accession

Primary (citable) accession number: Q3K6J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 8, 2005
Last modified:	January 19, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)