Q3K6H7 (RLMG_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	Pfl01_4890

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366477

Sequences

	Sequence		Length	Mass (Da)
	Q3K6H7 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: D3B13385514FCB34	FASTA	374	40,426
	10 20 30 40 50 60 MPLLDTPFAQ LDLIRQPEQQ NEPLQAFDAA DEYLLNHLAE QNLPIETRVL VLNDSFGALA 70 80 90 100 110 120 ISLSGNVQVS TSGDSFLAFQ GLEKNLLRNG QAFDALRGIP ASEPLVGPFD RVLIRVPKTL 130 140 150 160 170 180 ALLEEQLIRL QGQLAPGAQV VAAAMVKHLP RAAGDLLERY IGPVQASLAV KKARLLIATP 190 200 210 220 230 240 EAKAPAVSPY PTRYRLDEPA IELLNHANVF CREGLDIGTR AFLPHLPKNL GAAGVADLGC 250 260 270 280 290 300 GNGVLAIASA LQNPDAHYTL VDESFMAVQS AAENWRAALG EREAIVRAGD GLAGQEAQSL 310 320 330 340 350 360 DVVLCNPPFH QQQVVGDFLA WRMFQQAREA LVVGGALYIV GNRHLGYHSK LARLFRGVEQ 370 VAATPKFVIL KARK

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf0-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA76627.1.
RefSeq	YP_350618.1. NC_007492.2.
3D structure databases
ProteinModelPortal	Q3K6H7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3K6H7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3714247.
GenomeReviews	Gene locus Pfl01_4890 in contig CP000094_GR.
KEGG	pfo:Pfl01_4890.
PATRIC	19891976. VBIPseFlu44242_4903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	NRHLGYH.
ProtClustDB	CLSK868782.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_4890-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_PSEPF

Accession

Primary (citable) accession number: Q3K6H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program