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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPFLU205922:GJBD-5036-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Pfl01_4957
OrganismiPseudomonas fluorescens (strain Pf0-1)
Taxonomic identifieri205922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002704 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 868868Leucine--tRNA ligasePRO_1000202226Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi205922.Pfl01_4957.

Structurei

3D structure databases

ProteinModelPortaliQ3K6B0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi627 – 6315"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3K6B0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHEQYQPREI EAAAQSFWDE QKSFEVSEQP GKETYYCLSM FPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VISRYQRMLG KNVLQPMGWD AFGMPAENAA MKNNVAPAKW
110 120 130 140 150
TYENIAYMKS QLRSLGLAVD WSREVTTCKP DYYRWEQWLF TRLFEKGVIY
160 170 180 190 200
RKNGTVNWDP IDQTVLANEQ VIDGRGWRSG ALIEKREIPM YYFKITAYAD
210 220 230 240 250
ELLESLDELT GWPEQVKTMQ RNWIGKSRGM EVQFPYNVDS IGESGTLKVF
260 270 280 290 300
TTRPDTLMGA TYVAVAAEHH LAALAAKNNP ELQAFIAECK GGSVAEADVA
310 320 330 340 350
TQEKKGLPTG LFVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF
360 370 380 390 400
EFAHKYNLPV KSVVRTSSGD TNPAPWQDAY GEHGTLINSG EFDGLDFAGA
410 420 430 440 450
FDAMEVALIK KNLGASRTQF RLRDWGISRQ RYWGCPIPII HCDACGDVPV
460 470 480 490 500
PEDQLPVVLP EDVVPDGAGS PLARMPEFYE CTCPKCGQPA KRETDTMDTF
510 520 530 540 550
VESSWYYARY ASPHFEGGLV EKSAADHWLP VDQYIGGIEH AILHLLYARF
560 570 580 590 600
FHKLMRDEGL VSSNEPFKNL LTQGMVVAET YYRREANGAY TWFNPADVEL
610 620 630 640 650
ERDSKAKVIS AKLIADGLPV EIGGTEKMAK SKNNGVDPQS MIDQFGADTC
660 670 680 690 700
RLFMMFASPP DMSAEWSDSG VEGSHRFLKR VWRLAQAHIT QGLPGKLDIA
710 720 730 740 750
SLNDEQKVIR RAIHQAIKQA SHDVGQNHKF NTAIAQVMTL MNVLEKAAQA
760 770 780 790 800
TEQDRALVQE GLETVTLLLA PITPHISHEL WNRLGHADPV IDASWPVLDE
810 820 830 840 850
SALVQDSLTL VIQVNGKLRG QIEMPAAATR EEVEAAARAN ENVLRFVDGL
860
TIRKVIVVPG KLVNIVAS
Length:868
Mass (Da):97,095
Last modified:November 8, 2005 - v1
Checksum:i462BFB25F6E27EE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA. Translation: ABA76694.1.
RefSeqiWP_011336089.1. NC_007492.2.

Genome annotation databases

EnsemblBacteriaiABA76694; ABA76694; Pfl01_4957.
KEGGipfo:Pfl01_4957.
PATRICi19892112. VBIPseFlu44242_4971.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA. Translation: ABA76694.1.
RefSeqiWP_011336089.1. NC_007492.2.

3D structure databases

ProteinModelPortaliQ3K6B0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi205922.Pfl01_4957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA76694; ABA76694; Pfl01_4957.
KEGGipfo:Pfl01_4957.
PATRICi19892112. VBIPseFlu44242_4971.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPFLU205922:GJBD-5036-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf0-1.

Entry informationi

Entry nameiSYL_PSEPF
AccessioniPrimary (citable) accession number: Q3K6B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: November 8, 2005
Last modified: July 22, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.