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Q3K6B0 (SYL_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Pfl01_4957
OrganismPseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]
Taxonomic identifier205922 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length868 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 868868Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000202226

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif627 – 6315"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3K6B0 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 462BFB25F6E27EE4

FASTA86897,095
        10         20         30         40         50         60 
MHEQYQPREI EAAAQSFWDE QKSFEVSEQP GKETYYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPMGWD AFGMPAENAA MKNNVAPAKW TYENIAYMKS QLRSLGLAVD 

       130        140        150        160        170        180 
WSREVTTCKP DYYRWEQWLF TRLFEKGVIY RKNGTVNWDP IDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALIEKREIPM YYFKITAYAD ELLESLDELT GWPEQVKTMQ RNWIGKSRGM EVQFPYNVDS 

       250        260        270        280        290        300 
IGESGTLKVF TTRPDTLMGA TYVAVAAEHH LAALAAKNNP ELQAFIAECK GGSVAEADVA 

       310        320        330        340        350        360 
TQEKKGLPTG LFVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF EFAHKYNLPV 

       370        380        390        400        410        420 
KSVVRTSSGD TNPAPWQDAY GEHGTLINSG EFDGLDFAGA FDAMEVALIK KNLGASRTQF 

       430        440        450        460        470        480 
RLRDWGISRQ RYWGCPIPII HCDACGDVPV PEDQLPVVLP EDVVPDGAGS PLARMPEFYE 

       490        500        510        520        530        540 
CTCPKCGQPA KRETDTMDTF VESSWYYARY ASPHFEGGLV EKSAADHWLP VDQYIGGIEH 

       550        560        570        580        590        600 
AILHLLYARF FHKLMRDEGL VSSNEPFKNL LTQGMVVAET YYRREANGAY TWFNPADVEL 

       610        620        630        640        650        660 
ERDSKAKVIS AKLIADGLPV EIGGTEKMAK SKNNGVDPQS MIDQFGADTC RLFMMFASPP 

       670        680        690        700        710        720 
DMSAEWSDSG VEGSHRFLKR VWRLAQAHIT QGLPGKLDIA SLNDEQKVIR RAIHQAIKQA 

       730        740        750        760        770        780 
SHDVGQNHKF NTAIAQVMTL MNVLEKAAQA TEQDRALVQE GLETVTLLLA PITPHISHEL 

       790        800        810        820        830        840 
WNRLGHADPV IDASWPVLDE SALVQDSLTL VIQVNGKLRG QIEMPAAATR EEVEAAARAN 

       850        860 
ENVLRFVDGL TIRKVIVVPG KLVNIVAS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000094 Genomic DNA. Translation: ABA76694.1.
RefSeqYP_350685.1. NC_007492.2.

3D structure databases

ProteinModelPortalQ3K6B0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING205922.Pfl01_4957.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA76694; ABA76694; Pfl01_4957.
GeneID3715876.
KEGGpfo:Pfl01_4957.
PATRIC19892112. VBIPseFlu44242_4971.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPFLU205922:GJBD-5036-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PSEPF
AccessionPrimary (citable) accession number: Q3K6B0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: November 8, 2005
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries