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Protein

6,7-dimethyl-8-ribityllumazine synthase

Gene

ribH

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.UniRule annotationSAAS annotation

Catalytic activityi

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate.UniRule annotationSAAS annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 6,7-dimethyl-8-ribityllumazine synthase (ribH), 6,7-dimethyl-8-ribityllumazine synthase (ribH)
  2. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei245-amino-6-(D-ribitylamino)uracilUniRule annotation1
Active sitei90Proton donorUniRule annotation1
Binding sitei1155-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei1291-deoxy-L-glycero-tetrulose 4-phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotationImported
Biological processRiboflavin biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00275; UER00404

Names & Taxonomyi

Protein namesi
Recommended name:
6,7-dimethyl-8-ribityllumazine synthaseUniRule annotationSAAS annotation (EC:2.5.1.78UniRule annotationSAAS annotation)
Short name:
DMRL synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lumazine synthaseUniRule annotation
Gene namesi
Name:ribHUniRule annotationImported
Ordered Locus Names:Pfl01_5016Imported
OrganismiPseudomonas fluorescens (strain Pf0-1)Imported
Taxonomic identifieri205922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002704 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Proteomic databases

PRIDEiQ3K651

Interactioni

Subunit structurei

Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.UniRule annotation

Protein-protein interaction databases

STRINGi205922.Pfl01_5016

Structurei

3D structure databases

ProteinModelPortaliQ3K651
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 605-amino-6-(D-ribitylamino)uracil bindingUniRule annotation3
Regioni82 – 845-amino-6-(D-ribitylamino)uracil bindingUniRule annotation3
Regioni87 – 881-deoxy-L-glycero-tetrulose 4-phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DMRL synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4108UTT Bacteria
COG0054 LUCA
HOGENOMiHOG000229249
KOiK00794
OMAiHGNKGTE
OrthoDBiPOG091H021R

Family and domain databases

CDDicd09209 Lumazine_synthase-I, 1 hit
Gene3Di3.40.50.960, 1 hit
HAMAPiMF_00178 Lumazine_synth, 1 hit
InterProiView protein in InterPro
IPR034964 LS
IPR002180 LS/RS
IPR036467 LS/RS_sf
PANTHERiPTHR21058 PTHR21058, 1 hit
PfamiView protein in Pfam
PF00885 DMRL_synthase, 1 hit
SUPFAMiSSF52121 SSF52121, 1 hit
TIGRFAMsiTIGR00114 lumazine-synth, 1 hit

Sequencei

Sequence statusi: Complete.

Q3K651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLKTIEGTF IAPKGRYALV VGRFNSFVVE SLVSGAVDAL VRHGVSESDI
60 70 80 90 100
TIIRAPGAFE IPLVAQKVAQ KGEFAAIIAL GAVIRGGTPH FEYVAGECTK
110 120 130 140 150
GLAQVSMEFG VPVAFGVLTV DSIEQAIERS GTKAGNKGAE AALSALEMVS

LLAQLEAK
Length:158
Mass (Da):16,418
Last modified:November 8, 2005 - v1
Checksum:iEFE4AE7AE2501CB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000094 Genomic DNA Translation: ABA76753.1
RefSeqiWP_003228649.1, NC_007492.2

Genome annotation databases

EnsemblBacteriaiABA76753; ABA76753; Pfl01_5016
GeneIDi32070955
KEGGipfo:Pfl01_5016

Similar proteinsi

Entry informationi

Entry nameiQ3K651_PSEPF
AccessioniPrimary (citable) accession number: Q3K651
Entry historyiIntegrated into UniProtKB/TrEMBL: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health