Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q3K651 (Q3K651_PSEPF) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
6,7-dimethyl-8-ribityllumazine synthase HAMAP-Rule MF_00178

Short name=DMRL synthase HAMAP-Rule MF_00178
Short name=LS HAMAP-Rule MF_00178
Short name=Lumazine synthase HAMAP-Rule MF_00178
EC=2.5.1.78 HAMAP-Rule MF_00178
Gene names
Name:ribH HAMAP-Rule MF_00178 EMBL ABA76753.1
Ordered Locus Names:Pfl01_5016 EMBL ABA76753.1
OrganismPseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP] EMBL ABA76753.1
Taxonomic identifier205922 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length158 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin By similarity. HAMAP-Rule MF_00178 SAAS SAAS002180

Catalytic activity

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate. HAMAP-Rule MF_00178 RuleBase RU003795

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2. HAMAP-Rule MF_00178 RuleBase RU003795 SAAS SAAS002180

Subunit structure

Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers By similarity. HAMAP-Rule MF_00178

Sequence similarities

Belongs to the DMRL synthase family. HAMAP-Rule MF_00178 RuleBase RU003795

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region58 – 6035-amino-6-(D-ribitylamino)uracil binding By similarity HAMAP-Rule MF_00178
Region82 – 8435-amino-6-(D-ribitylamino)uracil binding By similarity HAMAP-Rule MF_00178
Region87 – 8821-deoxy-L-glycero-tetrulose 4-phosphate binding By similarity HAMAP-Rule MF_00178

Sites

Active site901Proton donor By similarity HAMAP-Rule MF_00178
Binding site2415-amino-6-(D-ribitylamino)uracil By similarity HAMAP-Rule MF_00178
Binding site11515-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygen By similarity HAMAP-Rule MF_00178
Binding site12911-deoxy-L-glycero-tetrulose 4-phosphate By similarity HAMAP-Rule MF_00178

Sequences

Sequence LengthMass (Da)Tools
Q3K651 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: EFE4AE7AE2501CB0

FASTA15816,418
        10         20         30         40         50         60 
MTLKTIEGTF IAPKGRYALV VGRFNSFVVE SLVSGAVDAL VRHGVSESDI TIIRAPGAFE 

        70         80         90        100        110        120 
IPLVAQKVAQ KGEFAAIIAL GAVIRGGTPH FEYVAGECTK GLAQVSMEFG VPVAFGVLTV 

       130        140        150 
DSIEQAIERS GTKAGNKGAE AALSALEMVS LLAQLEAK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000094 Genomic DNA. Translation: ABA76753.1.
RefSeqYP_350744.1. NC_007492.2.

3D structure databases

ProteinModelPortalQ3K651.
SMRQ3K651. Positions 3-156.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING205922.Pfl01_5016.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA76753; ABA76753; Pfl01_5016.
GeneID3713750.
KEGGpfo:Pfl01_5016.
PATRIC19892230. VBIPseFlu44242_5030.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0054.
HOGENOMHOG000229249.
KOK00794.
OMAISRFNDF.
OrthoDBEOG6RC3WC.

Enzyme and pathway databases

BioCycPFLU205922:GJBD-5095-MONOMER.
UniPathwayUPA00275; UER00404.

Family and domain databases

Gene3D3.40.50.960. 1 hit.
HAMAPMF_00178. Lumazine_synth.
InterProIPR002180. DMRL_synthase.
[Graphical view]
PANTHERPTHR21058. PTHR21058. 1 hit.
PfamPF00885. DMRL_synthase. 1 hit.
[Graphical view]
SUPFAMSSF52121. SSF52121. 1 hit.
TIGRFAMsTIGR00114. lumazine-synth. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ3K651_PSEPF
AccessionPrimary (citable) accession number: Q3K651
Entry history
Integrated into UniProtKB/TrEMBL: November 8, 2005
Last sequence update: November 8, 2005
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)