Reviewed, UniProtKB/Swiss-Prot Q3K5U8 (GPH_PSEPF)

Names and origin

Protein names

Recommended name:
    Phosphoglycolate phosphatase
      Short name=PGPase
      Short name=PGP
    EC=3.1.3.18

Gene names

Ordered Locus Names:

Pfl01_5119

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the dephosphorylation of 2-phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress By similarity.
Catalytic activity	2-phosphoglycolate + H2O = glycolate + phosphate. HAMAP MF_00495
Pathway	Organic acid metabolism; glycolic acid biosynthesis; glycolic acid from 2-phosphoglycolic acid: step 1/1. HAMAP MF_00495
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. CbbY/cbbZ/gph/yieH family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Phosphoglycolate phosphatase HAMAP MF_00495		PRO_0000238167
Sites
	Active site	19	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3K5U8-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 0E4BC755EE6F830D	FASTA	272	29,558
	10 20 30 40 50 60 MSGFEQLFPG QLPRLVMFDL DGTLIDSVPD LAAAVDNMLL SLGRKPAGIE SVREWVGNGA 70 80 90 100 110 120 PVLVRRALAG GIDHSSVDDV EAEHALEVFM EAYGASHELT VVYPGVRDTL KWLHKQGVAM 130 140 150 160 170 180 ALITNKPERF VAPLLDQMKI GRYFKWIIGG DTLPQKKPDP AALFFVMKMS GIPASQSLFV 190 200 210 220 230 240 GDSRSDVLAA KAAGVKCVGL SYGYNHGRPI AEESPTLVID DLRKLIPGCL DTAAEITLPD 250 260 270 AAQSPSGNAI VVVTRKLWMK VIKALARWRW RA

References

[1]

"Complete sequence of Pseudomonas fluorescens PfO-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyripides N., Anderson I., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA76856.1.
RefSeq	YP_350847.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3717037.
GenomeReviews	Gene locus Pfl01_5119 in contig CP000094_GR.
KEGG	pfo:Pfl01_5119.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3K5U8.
OMA	Q3K5U8. DSSNDAQ.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_5119-MON.
Family and domain databases
HAMAP	MF_00495. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR006439. HAD-SF_hydro_IA_v1. IPR006402. HAD-SF_hydro_IA_v3. IPR005833. Haloacid_DH/epoxide_hydro. IPR006346. PGP_bact. [Graphical view]
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00413. HADHALOGNASE.
TIGRFAMs	TIGR01549. HAD-SF-IA-v1. 1 hit. TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01449. PGP_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPH_PSEPF

Accession

Primary (citable) accession number: Q3K5U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)