Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q3K5N4 (MASZ_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase G
EC=2.3.3.9
Gene names
Name:glcB
Ordered Locus Names:Pfl01_5183
OrganismPseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]
Taxonomic identifier205922 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length725 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00641

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00641.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 725725Malate synthase G HAMAP-Rule MF_00641
PRO_1000056920

Sites

Active site3401Proton acceptor By similarity
Active site6311Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3K5N4 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: E1F9A076A9F8C674

FASTA72578,849
        10         20         30         40         50         60 
MTEHVQVGGL QVAKVLFDFV NNEAIPGTGL TADKFWEGAD KVIHDLAPKN KALLAKRDDF 

        70         80         90        100        110        120 
QARIDGWHQS RAGQPHDAVA YKAFLQEIGY LLPEAADFQA TTQNVDDEIA RTAGPQLVVP 

       130        140        150        160        170        180 
VMNARFALNA SNARWGSLYD ALYGTDAISE AGGAEKGKGY NKVRGDKVIA FARAFLDEAA 

       190        200        210        220        230        240 
PLATGSHVDS TGYKIADGKL VVTLKGGSTS GLRNDAQLIG FQGDANAPIA ILLKNNGLHF 

       250        260        270        280        290        300 
EIQIDASTPV GQTDAAGVKD ILMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 

       310        320        330        340        350        360 
AESVSKGGQT FTRTMNPDRT YTKVDGSELT LHGRSLLFVR NVGHLMTIDA ILDKHGNEVP 

       370        380        390        400        410        420 
EGILDGLVTC LAAMHNLSGN TSRKNTRTGS VYIVKPKMHG PEEAAFTNEL FGRIEDVLGL 

       430        440        450        460        470        480 
KRNTLKVGIM DEERRTTVNL KACIQAASER VVFINTGFLD RTGDEIHTSM EAGPMVRKAD 

       490        500        510        520        530        540 
MKAEKWIGAY ENWNVDIGLS TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV 

       550        560        570        580        590        600 
PSPTAAALHA LHYHKVDVFA RQAELAKRAR ASVDDILTIP LAVNPNWTPE QIKNELDNNA 

       610        620        630        640        650        660 
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ HIANWLRHGV VTEAQVMESL 

       670        680        690        700        710        720 
KRMAPVVDRQ NANDPLYRPL APNFDSNIAF QAAVELVIEG TKQPNGYTEP VLHRRRREFK 


AANGL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000094 Genomic DNA. Translation: ABA76920.1.
RefSeqYP_350911.1. NC_007492.2.

3D structure databases

ProteinModelPortalQ3K5N4.
SMRQ3K5N4. Positions 2-721.
ModBaseSearch...

Protein-protein interaction databases

STRING205922.Pfl01_5183.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA76920; ABA76920; Pfl01_5183.
GeneID3716773.
KEGGpfo:Pfl01_5183.
PATRIC19892586. VBIPseFlu44242_5198.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2225.
HOGENOMHOG000220740.
KOK01638.
OMAVVKPKMH.
ProtClustDBPRK02999.

Enzyme and pathway databases

BioCycPFLU205922:GJBD-5272-MONOMER.
UniPathwayUPA00703; UER00720.

Family and domain databases

Gene3D2.170.170.11. 2 hits.
HAMAPMF_00641. Malate_synth_G.
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PANTHERPTHR21631:SF1. PTHR21631:SF1. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. Malat_synth_like. 1 hit.
TIGRFAMsTIGR01345. malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_PSEPF
AccessionPrimary (citable) accession number: Q3K5N4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents