UniProtKB - Q3K5N4 (MASZ_PSEPF)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Malate synthase G
Gene
glcB
Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Functioni
Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation
Catalytic activityi
Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation
Cofactori
Mg2+UniRule annotation
: glyoxylate cycle Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Malate synthase G (glcB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 118 | Acetyl-CoA; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 276 | Acetyl-CoAUniRule annotation | 1 | |
Binding sitei | 313 | Acetyl-CoAUniRule annotation | 1 | |
Active sitei | 340 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 340 | GlyoxylateUniRule annotation | 1 | |
Metal bindingi | 432 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 432 | GlyoxylateUniRule annotation | 1 | |
Metal bindingi | 460 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 541 | Acetyl-CoA; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 631 | Proton donorUniRule annotation | 1 |
GO - Molecular functioni
- malate synthase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- glyoxylate cycle Source: UniProtKB-UniPathway
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | PFLU205922:G1G4S-5296-MONOMER. |
UniPathwayi | UPA00703; UER00720. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:glcBUniRule annotation Ordered Locus Names:Pfl01_5183 |
Organismi | Pseudomonas fluorescens (strain Pf0-1) |
Taxonomic identifieri | 205922 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000056920 | 1 – 725 | Malate synthase GAdd BLAST | 725 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 617 | Cysteine sulfenic acid (-SOH)UniRule annotation | 1 |
Keywords - PTMi
OxidationProteomic databases
PRIDEi | Q3K5N4. |
Interactioni
Subunit structurei
Monomer.UniRule annotation
Protein-protein interaction databases
STRINGi | 205922.Pfl01_5183. |
Structurei
3D structure databases
ProteinModelPortali | Q3K5N4. |
SMRi | Q3K5N4. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 125 – 126 | Acetyl-CoA bindingUniRule annotation | 2 | |
Regioni | 457 – 460 | Glyoxylate bindingUniRule annotation | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4107QP3. Bacteria. COG2225. LUCA. |
HOGENOMi | HOG000220740. |
KOi | K01638. |
OMAi | TEPVLHA. |
OrthoDBi | POG091H05P0. |
Family and domain databases
CDDi | cd00728. malate_synt_G. 1 hit. |
Gene3Di | 2.170.170.11. 1 hit. |
HAMAPi | MF_00641. Malate_synth_G. 1 hit. |
InterProi | View protein in InterPro IPR011076. Malate_synth-like_sf. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. |
Pfami | View protein in Pfam PF01274. Malate_synthase. 1 hit. |
SUPFAMi | SSF51645. SSF51645. 1 hit. |
TIGRFAMsi | TIGR01345. malate_syn_G. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q3K5N4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEHVQVGGL QVAKVLFDFV NNEAIPGTGL TADKFWEGAD KVIHDLAPKN
60 70 80 90 100
KALLAKRDDF QARIDGWHQS RAGQPHDAVA YKAFLQEIGY LLPEAADFQA
110 120 130 140 150
TTQNVDDEIA RTAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDAISE
160 170 180 190 200
AGGAEKGKGY NKVRGDKVIA FARAFLDEAA PLATGSHVDS TGYKIADGKL
210 220 230 240 250
VVTLKGGSTS GLRNDAQLIG FQGDANAPIA ILLKNNGLHF EIQIDASTPV
260 270 280 290 300
GQTDAAGVKD ILMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL
310 320 330 340 350
AESVSKGGQT FTRTMNPDRT YTKVDGSELT LHGRSLLFVR NVGHLMTIDA
360 370 380 390 400
ILDKHGNEVP EGILDGLVTC LAAMHNLSGN TSRKNTRTGS VYIVKPKMHG
410 420 430 440 450
PEEAAFTNEL FGRIEDVLGL KRNTLKVGIM DEERRTTVNL KACIQAASER
460 470 480 490 500
VVFINTGFLD RTGDEIHTSM EAGPMVRKAD MKAEKWIGAY ENWNVDIGLS
510 520 530 540 550
TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAAALHA
560 570 580 590 600
LHYHKVDVFA RQAELAKRAR ASVDDILTIP LAVNPNWTPE QIKNELDNNA
610 620 630 640 650
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ HIANWLRHGV
660 670 680 690 700
VTEAQVMESL KRMAPVVDRQ NANDPLYRPL APNFDSNIAF QAAVELVIEG
710 720
TKQPNGYTEP VLHRRRREFK AANGL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000094 Genomic DNA. Translation: ABA76920.1. |
RefSeqi | WP_011336251.1. NC_007492.2. |
Genome annotation databases
EnsemblBacteriai | ABA76920; ABA76920; Pfl01_5183. |
KEGGi | pfo:Pfl01_5183. |
Similar proteinsi
Entry informationi
Entry namei | MASZ_PSEPF | |
Accessioni | Q3K5N4Primary (citable) accession number: Q3K5N4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | November 8, 2005 | |
Last modified: | March 28, 2018 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |