Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q3K5N4

- MASZ_PSEPF

UniProt

Q3K5N4 - MASZ_PSEPF

Protein

Malate synthase G

Gene

glcB

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi432 – 4321MagnesiumUniRule annotation
    Binding sitei432 – 4321GlyoxylateUniRule annotation
    Metal bindingi460 – 4601MagnesiumUniRule annotation
    Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei631 – 6311Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciPFLU205922:GJBD-5272-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Pfl01_5183
    OrganismiPseudomonas fluorescens (strain Pf0-1)
    Taxonomic identifieri205922 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002704: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 725725Malate synthase GPRO_1000056920Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi205922.Pfl01_5183.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3K5N4.
    SMRiQ3K5N4. Positions 2-721.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni457 – 4604Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3K5N4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEHVQVGGL QVAKVLFDFV NNEAIPGTGL TADKFWEGAD KVIHDLAPKN    50
    KALLAKRDDF QARIDGWHQS RAGQPHDAVA YKAFLQEIGY LLPEAADFQA 100
    TTQNVDDEIA RTAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDAISE 150
    AGGAEKGKGY NKVRGDKVIA FARAFLDEAA PLATGSHVDS TGYKIADGKL 200
    VVTLKGGSTS GLRNDAQLIG FQGDANAPIA ILLKNNGLHF EIQIDASTPV 250
    GQTDAAGVKD ILMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 300
    AESVSKGGQT FTRTMNPDRT YTKVDGSELT LHGRSLLFVR NVGHLMTIDA 350
    ILDKHGNEVP EGILDGLVTC LAAMHNLSGN TSRKNTRTGS VYIVKPKMHG 400
    PEEAAFTNEL FGRIEDVLGL KRNTLKVGIM DEERRTTVNL KACIQAASER 450
    VVFINTGFLD RTGDEIHTSM EAGPMVRKAD MKAEKWIGAY ENWNVDIGLS 500
    TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAAALHA 550
    LHYHKVDVFA RQAELAKRAR ASVDDILTIP LAVNPNWTPE QIKNELDNNA 600
    QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ HIANWLRHGV 650
    VTEAQVMESL KRMAPVVDRQ NANDPLYRPL APNFDSNIAF QAAVELVIEG 700
    TKQPNGYTEP VLHRRRREFK AANGL 725
    Length:725
    Mass (Da):78,849
    Last modified:November 8, 2005 - v1
    Checksum:iE1F9A076A9F8C674
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000094 Genomic DNA. Translation: ABA76920.1.
    RefSeqiWP_011336251.1. NC_007492.2.
    YP_350911.1. NC_007492.2.

    Genome annotation databases

    EnsemblBacteriaiABA76920; ABA76920; Pfl01_5183.
    GeneIDi3716773.
    KEGGipfo:Pfl01_5183.
    PATRICi19892586. VBIPseFlu44242_5198.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000094 Genomic DNA. Translation: ABA76920.1 .
    RefSeqi WP_011336251.1. NC_007492.2.
    YP_350911.1. NC_007492.2.

    3D structure databases

    ProteinModelPortali Q3K5N4.
    SMRi Q3K5N4. Positions 2-721.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 205922.Pfl01_5183.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA76920 ; ABA76920 ; Pfl01_5183 .
    GeneIDi 3716773.
    KEGGi pfo:Pfl01_5183.
    PATRICi 19892586. VBIPseFlu44242_5198.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci PFLU205922:GJBD-5272-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pf0-1.

    Entry informationi

    Entry nameiMASZ_PSEPF
    AccessioniPrimary (citable) accession number: Q3K5N4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3