glcB

Functionⁱ

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityⁱ

Acetyl-CoA + H₂O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactorⁱ

Mg²⁺UniRule annotation

Pathwayⁱ: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

no protein annotated in this organism
Malate synthase G (glcB)

This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	118	Acetyl-CoA; via carbonyl oxygenUniRule annotation	1
Binding siteⁱ	276	Acetyl-CoAUniRule annotation	1
Binding siteⁱ	313	Acetyl-CoAUniRule annotation	1
Active siteⁱ	340	Proton acceptorUniRule annotation	1
Binding siteⁱ	340	GlyoxylateUniRule annotation	1
Metal bindingⁱ	432	MagnesiumUniRule annotation	1
Binding siteⁱ	432	GlyoxylateUniRule annotation	1
Metal bindingⁱ	460	MagnesiumUniRule annotation	1
Binding siteⁱ	541	Acetyl-CoA; via carbonyl oxygenUniRule annotation	1
Active siteⁱ	631	Proton donorUniRule annotation	1

GO - Molecular functionⁱ

malate synthase activity Source: UniProtKB-EC
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glyoxylate cycle Source: UniProtKB-UniPathway
tricarboxylic acid cycle Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Glyoxylate bypass, Tricarboxylic acid cycle
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BioCycⁱ	PFLU205922:G1G4S-5296-MONOMER
UniPathwayⁱ	UPA00703; UER00720

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesⁱ	Name:glcBUniRule annotation Ordered Locus Names:Pfl01_5183
Organismⁱ	Pseudomonas fluorescens (strain Pf0-1)
Taxonomic identifierⁱ	205922 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas fluorescens
Proteomesⁱ	UP000002704 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00641

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_1000056920	1 – 725	Malate synthase GAdd BLAST		725

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	617	Cysteine sulfenic acid (-SOH)UniRule annotation		1

Keywords - PTMⁱ

Oxidation

Proteomic databases

PRIDEⁱ	Q3K5N4

PRIDEⁱ

Q3K5N4

Interactionⁱ

Subunit structureⁱ

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	205922.Pfl01_5183

STRINGⁱ

205922.Pfl01_5183

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q3K5N4
SMRⁱ	Q3K5N4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	125 – 126	Acetyl-CoA bindingUniRule annotation		2
Regionⁱ	457 – 460	Glyoxylate bindingUniRule annotation		4

Sequence similaritiesⁱ

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4107QP3 Bacteria COG2225 LUCA
HOGENOMⁱ	HOG000220740
KEGG Orthology (KO) More...KOⁱ	K01638
OMAⁱ	TEPVLHA
Database of Orthologous Groups More...OrthoDBⁱ	POG091H05P0

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00728 malate_synt_G, 1 hit
Gene3Dⁱ	2.170.170.11, 1 hit
HAMAPⁱ	MF_00641 Malate_synth_G, 1 hit
InterProⁱ	View protein in InterPro IPR011076 Malate_synth-like_sf IPR023310 Malate_synth_G_beta_sub_dom IPR001465 Malate_synthase IPR006253 Malate_synthG
PANTHERⁱ	PTHR42739 PTHR42739, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01274 Malate_synthase, 1 hit
SUPFAMⁱ	SSF51645 SSF51645, 1 hit
TIGRFAMsⁱ	TIGR01345 malate_syn_G, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q3K5N4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTEHVQVGGL QVAKVLFDFV NNEAIPGTGL TADKFWEGAD KVIHDLAPKN 
        60         70         80         90        100
KALLAKRDDF QARIDGWHQS RAGQPHDAVA YKAFLQEIGY LLPEAADFQA 
       110        120        130        140        150
TTQNVDDEIA RTAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDAISE 
       160        170        180        190        200
AGGAEKGKGY NKVRGDKVIA FARAFLDEAA PLATGSHVDS TGYKIADGKL 
       210        220        230        240        250
VVTLKGGSTS GLRNDAQLIG FQGDANAPIA ILLKNNGLHF EIQIDASTPV 
       260        270        280        290        300
GQTDAAGVKD ILMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 
       310        320        330        340        350
AESVSKGGQT FTRTMNPDRT YTKVDGSELT LHGRSLLFVR NVGHLMTIDA 
       360        370        380        390        400
ILDKHGNEVP EGILDGLVTC LAAMHNLSGN TSRKNTRTGS VYIVKPKMHG 
       410        420        430        440        450
PEEAAFTNEL FGRIEDVLGL KRNTLKVGIM DEERRTTVNL KACIQAASER 
       460        470        480        490        500
VVFINTGFLD RTGDEIHTSM EAGPMVRKAD MKAEKWIGAY ENWNVDIGLS 
       510        520        530        540        550
TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAAALHA 
       560        570        580        590        600
LHYHKVDVFA RQAELAKRAR ASVDDILTIP LAVNPNWTPE QIKNELDNNA 
       610        620        630        640        650
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ HIANWLRHGV 
       660        670        680        690        700
VTEAQVMESL KRMAPVVDRQ NANDPLYRPL APNFDSNIAF QAAVELVIEG 
       710        720 
TKQPNGYTEP VLHRRRREFK AANGL

Length:725

Mass (Da):78,849

Last modified:November 8, 2005 - v1

Checksum:ⁱE1F9A076A9F8C674

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000094 Genomic DNA Translation: ABA76920.1
NCBI Reference Sequences More...RefSeqⁱ	WP_011336251.1, NC_007492.2

Genome annotation databases

EnsemblBacteriaⁱ	ABA76920; ABA76920; Pfl01_5183
KEGGⁱ	pfo:Pfl01_5183

Protein	Similar proteins		Species	Score	Length
Q3K5N4	Malate synthase G		PSEFL		725
Malate synthase G		Pseudomonas sp. MPR-R2A5		725
Malate synthase G		Pseudomonas sp. SHC52		749
Malate synthase G		Pseudomonas sp. GM50		730
Malate synthase G		Pseudomonas frederiksbergensis		730
+505

Protein	Similar proteins		Species	Score	Length
Q3K5N4	Malate synthase G		CORGL		739
Malate synthase G		Corynebacterium crudilactis		744
Malate synthase G		CORGT		740
UPI00080749FF		Corynebacterium		739
Malate synthase G		CORGB		739
+3679

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000094 Genomic DNA Translation: ABA76920.1
NCBI Reference Sequences More...RefSeqⁱ	WP_011336251.1, NC_007492.2

3D structure databases

ProteinModelPortalⁱ	Q3K5N4
SMRⁱ	Q3K5N4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	205922.Pfl01_5183

STRINGⁱ

205922.Pfl01_5183

Proteomic databases

PRIDEⁱ	Q3K5N4

PRIDEⁱ

Q3K5N4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ABA76920; ABA76920; Pfl01_5183
KEGGⁱ	pfo:Pfl01_5183

Phylogenomic databases

eggNOGⁱ	ENOG4107QP3 Bacteria COG2225 LUCA
HOGENOMⁱ	HOG000220740
KEGG Orthology (KO) More...KOⁱ	K01638
OMAⁱ	TEPVLHA
Database of Orthologous Groups More...OrthoDBⁱ	POG091H05P0

Enzyme and pathway databases

UniPathwayⁱ	UPA00703; UER00720
BioCycⁱ	PFLU205922:G1G4S-5296-MONOMER

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00728 malate_synt_G, 1 hit
Gene3Dⁱ	2.170.170.11, 1 hit
HAMAPⁱ	MF_00641 Malate_synth_G, 1 hit
InterProⁱ	View protein in InterPro IPR011076 Malate_synth-like_sf IPR023310 Malate_synth_G_beta_sub_dom IPR001465 Malate_synthase IPR006253 Malate_synthG
PANTHERⁱ	PTHR42739 PTHR42739, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01274 Malate_synthase, 1 hit
SUPFAMⁱ	SSF51645 SSF51645, 1 hit
TIGRFAMsⁱ	TIGR01345 malate_syn_G, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MASZ_PSEPF
Accessionⁱ	Q3K5N4Primary (citable) accession number: Q3K5N4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
	Last sequence update:	November 8, 2005
	Last modified:	June 20, 2018
	This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q3K5N4 (MASZ_PSEPF)

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Malate synthase G

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glyoxylate cycle

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: glyoxylate cycle

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ