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Q3K5K9

- GLYA3_PSEPF

UniProt

Q3K5K9 - GLYA3_PSEPF

Protein

Serine hydroxymethyltransferase 3

Gene

glyA3

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei35 – 351Pyridoxal phosphateUniRule annotation
    Binding sitei55 – 551Pyridoxal phosphateUniRule annotation
    Binding sitei57 – 571SubstrateUniRule annotation
    Binding sitei64 – 641SubstrateUniRule annotation
    Binding sitei65 – 651Pyridoxal phosphateUniRule annotation
    Binding sitei99 – 991Pyridoxal phosphateUniRule annotation
    Binding sitei121 – 1211Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei176 – 1761Pyridoxal phosphateUniRule annotation
    Binding sitei204 – 2041Pyridoxal phosphateUniRule annotation
    Binding sitei229 – 2291Pyridoxal phosphateUniRule annotation
    Binding sitei236 – 2361Pyridoxal phosphateUniRule annotation
    Binding sitei263 – 2631Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Binding sitei363 – 3631Pyridoxal phosphateUniRule annotation

    GO - Molecular functioni

    1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciPFLU205922:GJBD-5297-MONOMER.
    UniPathwayiUPA00193.
    UPA00288; UER01023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine hydroxymethyltransferase 3UniRule annotation (EC:2.1.2.1UniRule annotation)
    Short name:
    SHMT 3UniRule annotation
    Short name:
    Serine methylase 3UniRule annotation
    Gene namesi
    Name:glyA3UniRule annotation
    Ordered Locus Names:Pfl01_5208
    OrganismiPseudomonas fluorescens (strain Pf0-1)
    Taxonomic identifieri205922 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002704: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 417417Serine hydroxymethyltransferase 3PRO_0000235006Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei230 – 2301N6-(pyridoxal phosphate)lysineUniRule annotation

    Proteomic databases

    PRIDEiQ3K5K9.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi205922.Pfl01_5208.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3K5K9.
    SMRiQ3K5K9. Positions 1-416.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1273Substrate bindingUniRule annotation
    Regioni355 – 3573Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the SHMT family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0112.
    HOGENOMiHOG000239404.
    KOiK00600.
    OMAiQAMTLEF.
    OrthoDBiEOG6Z0QB2.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00051. SHMT.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view]
    PANTHERiPTHR11680. PTHR11680. 1 hit.
    PfamiPF00464. SHMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000412. SHMT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00096. SHMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3K5K9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSKQDQIQG YDDALLAAMN AEEQRQEDHI ELIASENYTS KRVMQAQGSG    50
    LTNKYAEGYP GKRYYGGCEH VDKVEALAIE RAKQLFGADY ANVQPHSGSS 100
    ANSAVYLALL QAGDTILGMS LAHGGHLTHG AKVSSSGKLY NAVQYGIDTK 150
    TGLIDYDEVE RLAVECKPKM IVAGFSAYSK TLDFPRFRQI ADKVGALLFV 200
    DMAHVAGLVA AGLYPNPLPY ADVVTTTTHK TLRGPRGGLI LAKANEEIEK 250
    KLNAAVFPGA QGGPLMHVIA GKAVCFKEAL EPGFKAYQQQ VIDNAQAMAS 300
    VFIKRGYDVV SGGTDNHLFL VSLIRQGLTG KDADAALGRA HITVNKNAVP 350
    NDPQSPFVTS GLRIGTPAVT TRGFKVTQCV TLAGWICDIL DNLGDADVEA 400
    NVAQQVSALC ADFPVYR 417
    Length:417
    Mass (Da):44,656
    Last modified:November 8, 2005 - v1
    Checksum:i0CF0D3E856516329
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000094 Genomic DNA. Translation: ABA76945.1.
    RefSeqiWP_011336274.1. NC_007492.2.
    YP_350936.1. NC_007492.2.

    Genome annotation databases

    EnsemblBacteriaiABA76945; ABA76945; Pfl01_5208.
    GeneIDi3716531.
    KEGGipfo:Pfl01_5208.
    PATRICi19892636. VBIPseFlu44242_5223.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000094 Genomic DNA. Translation: ABA76945.1 .
    RefSeqi WP_011336274.1. NC_007492.2.
    YP_350936.1. NC_007492.2.

    3D structure databases

    ProteinModelPortali Q3K5K9.
    SMRi Q3K5K9. Positions 1-416.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 205922.Pfl01_5208.

    Proteomic databases

    PRIDEi Q3K5K9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA76945 ; ABA76945 ; Pfl01_5208 .
    GeneIDi 3716531.
    KEGGi pfo:Pfl01_5208.
    PATRICi 19892636. VBIPseFlu44242_5223.

    Phylogenomic databases

    eggNOGi COG0112.
    HOGENOMi HOG000239404.
    KOi K00600.
    OMAi QAMTLEF.
    OrthoDBi EOG6Z0QB2.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    UPA00288 ; UER01023 .
    BioCyci PFLU205922:GJBD-5297-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00051. SHMT.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view ]
    PANTHERi PTHR11680. PTHR11680. 1 hit.
    Pfami PF00464. SHMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000412. SHMT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00096. SHMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pf0-1.

    Entry informationi

    Entry nameiGLYA3_PSEPF
    AccessioniPrimary (citable) accession number: Q3K5K9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3