Reviewed, UniProtKB/Swiss-Prot Q3K5H4 (BETB_PSEPF)

Names and origin

Protein names

Recommended name:
    Betaine aldehyde dehydrogenase
      Short name=BADH
    EC=1.2.1.8

Gene names

Name:	betB
Ordered Locus Names:	Pfl01_5243

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Betaine aldehyde + NAD+ + H2O = betaine + NADH. HAMAP MF_00804
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1. HAMAP MF_00804
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	betaine-aldehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Betaine aldehyde dehydrogenase HAMAP MF_00804		PRO_1000047050
Regions
	Nucleotide binding	229 – 234	6	NAD By similarity
Sites
	Active site	252	1	By similarity
	Active site	286	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3K5H4-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: C4D0AB6F274B3D75	FASTA	490	53,174
	10 20 30 40 50 60 MARFELQKLY IDGAYSDAGS DATFEAINPA NGEVLAHVQR ATKEDVERAV VSAEKGQKIW 70 80 90 100 110 120 AAMTAMERSR ILRRAVDILR ERNDELAALE TLDTGKAFSE TKYVDIVTGA DVLEYYAGLV 130 140 150 160 170 180 PAIEGEQIPL RDTSFVYTRR EPLGVVAGIG AWNYPIQIAL WKSAPALAAG NAMIFKPSEV 190 200 210 220 230 240 TSLTTLKLAE IYTEAGVPNG VFNVLTGSGR EVGTWLTEHP RIEKISFTGG TDTGKKVMAS 250 260 270 280 290 300 ASASSLKDVT MELGGKSPLI ICDDADLDRA ADTAMMANFY SSGQVCTNGT RVFVPSHLKA 310 320 330 340 350 360 AFEAKIVERV ARIRVGNPED ENTNFGPLVS FPHMESVLGY IAKGKEEGAR VLCGGERLTD 370 380 390 400 410 420 GEFAKGAFVA PTVFTDCTDD MTIVREEIFG PVMAILSYET EEEVIRRAND TDFGLAAGIV 430 440 450 460 470 480 TRDLNRAHRV IHQLEAGICW INAWGESDAK MPVGGYKQSG VGRENGISSL NNFTRIKSVQ 490 VELGDYVSVF

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA76980.1.
RefSeq	YP_350971.1.
3D structure databases
HSSP	HSSP built from PDB template 1BPW based on UniProtKB P56533.
SMR	Q3K5H4. Positions 8-490.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3K5H4.
Genome annotation databases
GeneID	3716194.
GenomeReviews	Gene locus Pfl01_5243 in contig CP000094_GR.
KEGG	pfo:Pfl01_5243.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HBG752218.
OMA	EPLGLCV.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_5243-MONOMER.
Family and domain databases
HAMAP	MF_00804. BADH. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR011264. Betaine_Ald_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01804. BADH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETB_PSEPF

Accession

Primary (citable) accession number: Q3K5H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)