Reviewed, UniProtKB/Swiss-Prot Q3K5H3 (BETA_PSEPF)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	Pfl01_5244

Organism

Pseudomonas fluorescens (strain PfO-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Choline dehydrogenase		PRO_0000258929
Regions
	Nucleotide binding	6 – 35	30	FAD Probable
Sites
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3K5H3-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 55298A5390F9725F	FASTA	567	62,522
	10 20 30 40 50 60 MSQEFDYIIV GAGSAGNTLA TRLTEDEGVT VLLLEAGGPD YRFDFRTQMP AALAFPLQGR 70 80 90 100 110 120 RYNWAYETDP EPHMDGRRME CGRGKGLGGS SLINGMCYIR GNAMDYDGWA KLPGLEDWSY 130 140 150 160 170 180 LDCLPYFRKA ETRDIGPNDY HGGDGPVSVT TPKAGNNPLF HAMVEAGVQA GYPRTEDLNG 190 200 210 220 230 240 YQQEGFGPMD RTVTPNGRRA STARGYLDVA KKRSTLTIVT HALTDKVLFE GKRAVGVRYL 250 260 270 280 290 300 VGAAEERVEA RARKEVIVCS GAIASPQLLQ RSGVGPAKLL ESLDIPVVHD LPGVGENLQD 310 320 330 340 350 360 HLELYLQYAC TQPVSLYPSL LWYNQPAIGA EWLFNGTGIG ASNQFEAGGF IRTRPEFEWP 370 380 390 400 410 420 NIQYHFLPVA INYNGSNGVK EHGFQAHMGS MRSPSRGRVQ VKSKDPRQHP SILFNYMATE 430 440 450 460 470 480 QDWQEFRDGI RLTREIMQQP ALDAFRGREI SPGIEVQTDE QLDKFIREHA ETAFHPSCSC 490 500 510 520 530 540 KMGTDDMAVV DGEGRVHGMQ GLRVVDASIM PIITTGNLNA PTIMMAEKIA DKIRGRQPLP 550 560 RSKAPYYVAG DAPVKGKAMR DVSAVAQ

References

[1]

"Complete sequence of Pseudomonas fluorescens PfO-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyripides N., Anderson I., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA76981.1.
RefSeq	YP_350972.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3716195.
GenomeReviews	Gene locus Pfl01_5244 in contig CP000094_GR.
KEGG	pfo:PflO1_5244.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3K5H3.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_5244-MON.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_PSEPF

Accession

Primary (citable) accession number: Q3K5H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 8, 2005
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)