Q3K588 (TRMB_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	Pfl01_5329

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas fluorescens

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000229186
Regions
	Region	219 – 222	4	Substrate binding Potential
Sites
	Binding site	71	1	S-adenosyl-L-methionine By similarity
	Binding site	96	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity
	Binding site	146	1	S-adenosyl-L-methionine By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	182	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3K588 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 5BB3DAD578FFA3EE	FASTA	241	27,163
	10 20 30 40 50 60 MTESNDTPIQ TEEGDERQHR RIKSFVMRAG RMTEGQQRGL DQGAPKFVLP LADAPVDFDQ 70 80 90 100 110 120 VFGRSAPRSL EIGFGMGHSL LEMAAAAPEQ DFIGVEVHRP GVGALLNGVL TQGLTNLRVY 130 140 150 160 170 180 DCDAIEVLNR CIADNSLDRL MLFFPDPWHK SRHHKRRIVQ ASFAELVRSK LKVGGILHMA 190 200 210 220 230 240 TDWEPYAEYM LEVMNVAPGY RNLAEDGKCV PRPAERPITK FERRGERLGH GVWDLKFEKL A

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf0-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA77066.1.
RefSeq	YP_351057.1. NC_007492.2.
3D structure databases
ProteinModelPortal	Q3K588.
ModBase	Search...
Protein-protein interaction databases
STRING	205922.Pfl01_5329.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABA77066; ABA77066; Pfl01_5329.
GeneID	3714898.
KEGG	pfo:Pfl01_5329.
PATRIC	19892880. VBIPseFlu44242_5345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000073968.
KO	K03439.
OMA	FIHCATD.
ProtClustDB	PRK00121.
Enzyme and pathway databases
BioCyc	PFLU205922:GJBD-5418-MONOMER.
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_PSEPF

Accession

Primary (citable) accession number: Q3K588

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program