Reviewed, UniProtKB/Swiss-Prot Q3K4Z8 (ARGA_PSEPF)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	Pfl01_5419

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Amino-acid acetyltransferase HAMAP MF_01105		PRO_1000084819
Regions
	Domain	286 – 425	140	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	Q3K4Z8-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 6A1EBC81BA6B9AC9	FASTA	432	47,697
	10 20 30 40 50 60 MPEYVNWLRH ASPYINAHRD CTFVVMLPGD GVEHPNFGNI VHDIVLLHSL GVRLVLVHGS 70 80 90 100 110 120 RPQIETRLAA RGLTPHYHHG MRITDAATLE CVIDAVGQLR IAIEARLSMD MASSPMQGSR 130 140 150 160 170 180 LRVASGNLVT ARPIGVLEGV DYHHTGEVRR VDRKGINRLL DERSIVLLSP LGYSPTGEIF 190 200 210 220 230 240 NLACEDVATR AAIDLGADKL LLFGADLGLI DENGKLVREL RPQQVPAHLQ RLGSNYQAEL 250 260 270 280 290 300 LDAAAEACRG GVARSHIVSY AEDGALLTEL FTRDGGGTLV AQEQFELVRE AAIEDVGGLL 310 320 330 340 350 360 DLISPLEEQG ILVRRSREVL EREIEQFSVV EREGMIIACA ALYQIADSDA GELACLAVNP 370 380 390 400 410 420 EYRHGGRGDE LLERIETRAR AQGLKTLFVL TTRTAHWFRE RGFEPSSVER LPAARASLYN 430 YQRNSKIFEK SL

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:RESEARCH51.1-RESEARCH51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA77156.1.
RefSeq	YP_351147.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3K4Z8.
Genome annotation databases
GeneID	3714441.
GenomeReviews	Gene locus Pfl01_5419 in contig CP000094_GR.
KEGG	pfo:Pfl01_5419.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3K4Z8.
OMA	DLIRPLE.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_5419-MON.
Family and domain databases
HAMAP	MF_01105. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_PSEPF

Accession

Primary (citable) accession number: Q3K4Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)