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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei258ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:SAK_0165
OrganismiStreptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
Taxonomic identifieri205921 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002374071 – 484Glutamate--tRNA ligaseAdd BLAST484

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ3K3R5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionAdd BLAST11
Motifi255 – 259"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252720.
KOiK09698.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3K3R5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANKIRVRYA PSPTGLLHIG NARTALFNYL YARHHGGDFV IRIEDTDRKR
60 70 80 90 100
HVEDGERSQL ENLRWLGMDW DESPETHENY RQSERLELYQ RYIDQLLAEG
110 120 130 140 150
KAYKSYVTEE ELAAERERQE LAGETPRYIN EFIGMSETEK EAYIAEREAA
160 170 180 190 200
GIIPTVRLAV SESGIYKWTD MVKGDIEFEG SNIGGDWVIQ KKDGYPTYNF
210 220 230 240 250
AVVIDDHVMQ ISHVIRGDDH IANTPKQLMV YEALGWEAPQ FGHMTLIINS
260 270 280 290 300
ETGKKLSKRD TNTLQFIEDY RKKGYMSEAV FNFIALLGWN PGGEEEIFSR
310 320 330 340 350
EQLINLFDEN RLSKSPAAFD QKKMDWMSND YLKNADFESV FALCKPFLEE
360 370 380 390 400
AGRLTDKAEK LVELYKPQLK SADEIVPLTD LFFADFPELT EAEKEVMAAE
410 420 430 440 450
TVPTVLSVFK EKLVSLSDEE FTRDTIFPQI KAVQKETGIK GKNLFMPIRI
460 470 480
AVSGEMHGPE LPDTIYLLGK EKSVQHIDNM LAKL
Length:484
Mass (Da):55,540
Last modified:March 24, 2009 - v2
Checksum:i867D06E1D632E906
GO

Sequence cautioni

The sequence ABA45477 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000114 Genomic DNA. Translation: ABA45477.1. Different initiation.
RefSeqiWP_001284504.1. NC_007432.1.

Genome annotation databases

EnsemblBacteriaiABA45477; ABA45477; SAK_0165.
KEGGisak:SAK_0165.
PATRICi19631530. VBIStrAga82541_0112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000114 Genomic DNA. Translation: ABA45477.1. Different initiation.
RefSeqiWP_001284504.1. NC_007432.1.

3D structure databases

ProteinModelPortaliQ3K3R5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA45477; ABA45477; SAK_0165.
KEGGisak:SAK_0165.
PATRICi19631530. VBIStrAga82541_0112.

Phylogenomic databases

HOGENOMiHOG000252720.
KOiK09698.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_STRA1
AccessioniPrimary (citable) accession number: Q3K3R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: March 24, 2009
Last modified: November 30, 2016
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.