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Q3K2N4

- SYI_STRA1

UniProt

Q3K2N4 - SYI_STRA1

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAGA205921:GHD7-587-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SAK_0587
OrganismiStreptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
Taxonomic identifieri205921 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002701: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Isoleucine--tRNA ligasePRO_0000098475Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi205921.SAK_0587.

Structurei

3D structure databases

ProteinModelPortaliQ3K2N4.
SMRiQ3K2N4. Positions 1-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3K2N4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLKETLNLG QTAFPMRAGL PNKEPQWQEA WDQADIYKKR QALNEGKPAF
60 70 80 90 100
HLHDGPPYAN GNIHVGHALN KISKDIIVRS KSMSGFRAPY VPGWDTHGLP
110 120 130 140 150
IEQVLAKKGV KRKEMDLAEY LEMCRDYALS QVDKQRDDFK RLGVSADWEN
160 170 180 190 200
PYITLTPDYE ADQVRVFGAM ADKGYIYRGA KPVYWSWSSE SALAEAEIEY
210 220 230 240 250
HDIDSTSLYY ANKVKDGKGI LDTDTYIVVW TTTPFTVTAS RGLTVGPDME
260 270 280 290 300
YVVVVPVGSE RKYLLAEVLV DSLAAKFGWE NFEIVTHHTG KELNHIVTEH
310 320 330 340 350
PWDTEVEELV ILGDHVTTDS GTGIVHTAPG FGEDDYNVGI ANGLDVVVTV
360 370 380 390 400
DSRGLMMENA GPDFEGQFYD KVTPLVKEKL GDLLLASEVI NHSYPFDWRT
410 420 430 440 450
KKPIIWRAVP QWFASVSKFR QEILDEIEKT NFQPEWGKKR LYNMIRDRGD
460 470 480 490 500
WVISRQRAWG VPLPIFYAED GTAIMTKEVT DHVADLFAEY GSIVWWQRDA
510 520 530 540 550
KDLLPAGYTH PGSPNGLFEK ETDIMDVWFD SGSSWNGVMN ARENLSYPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSLITS VAVNGHAPYK AVLSQGFVLD GKGEKMSKSL
610 620 630 640 650
GNTILPSDVE KQFGAEILRL WVTSVDSSND VRISMDILKQ TSETYRKIRN
660 670 680 690 700
TLRFLIANTS DFNPKQDAVA YENLGAVDRY MTIKFNQVVD TINKAYAAYD
710 720 730 740 750
FMAIYKAVVN FVTVDLSAFY LDFAKDVVYI EAANSPERRR MQTVFYDILV
760 770 780 790 800
KLTKLLTPIL PHTAEEIWSY LEHEEEEFVQ LAEMPVAQTF SGQEEILEEW
810 820 830 840 850
SAFMTLRTQA QKALEEARNA KVIGKSLEAH LTIYASQEVK TLLTALNSDI
860 870 880 890 900
ALLMIVSQLT IADEADKPAD SVSFEGVAFT VEHAEGEVCE RSRRIDPTTK
910 920 930
MRSYGVAVCD ASAAIIEQYY PEAVAQGFEA
Length:930
Mass (Da):104,897
Last modified:November 8, 2005 - v1
Checksum:iC77646FA00A10CDE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000114 Genomic DNA. Translation: ABA45845.1.
RefSeqiYP_329219.1. NC_007432.1.

Genome annotation databases

EnsemblBacteriaiABA45845; ABA45845; SAK_0587.
GeneIDi3686597.
KEGGisak:SAK_0587.
PATRICi19632401. VBIStrAga82541_0511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000114 Genomic DNA. Translation: ABA45845.1 .
RefSeqi YP_329219.1. NC_007432.1.

3D structure databases

ProteinModelPortali Q3K2N4.
SMRi Q3K2N4. Positions 1-918.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 205921.SAK_0587.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA45845 ; ABA45845 ; SAK_0587 .
GeneIDi 3686597.
KEGGi sak:SAK_0587.
PATRICi 19632401. VBIStrAga82541_0511.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SAGA205921:GHD7-587-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial 'pan-genome'."
    Tettelin H., Masignani V., Cieslewicz M.J., Donati C., Medini D., Ward N.L., Angiuoli S.V., Crabtree J., Jones A.L., Durkin A.S., DeBoy R.T., Davidsen T.M., Mora M., Scarselli M., Margarit y Ros I., Peterson J.D., Hauser C.R., Sundaram J.P.
    , Nelson W.C., Madupu R., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sullivan S.A., Daugherty S.C., Haft D.H., Selengut J., Gwinn M.L., Zhou L., Zafar N., Khouri H., Radune D., Dimitrov G., Watkins K., O'Connor K.J., Smith S., Utterback T.R., White O., Rubens C.E., Grandi G., Madoff L.C., Kasper D.L., Telford J.L., Wessels M.R., Rappuoli R., Fraser C.M.
    Proc. Natl. Acad. Sci. U.S.A. 102:13950-13955(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27591 / A909 / CDC SS700.

Entry informationi

Entry nameiSYI_STRA1
AccessioniPrimary (citable) accession number: Q3K2N4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3