Reviewed, UniProtKB/Swiss-Prot Q3K1L5 (MURA1_STRA1)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 1
    UDP-N-acetylglucosamine enolpyruvyl transferase 1
      Short name=EPT 1

Gene names

Name:	murA1
Ordered Locus Names:	SAK_0966

Organism

Streptococcus agalactiae serotype Ia [Complete proteome] [HAMAP]

Taxonomic identifier

355315 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity.
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 HAMAP MF_00111		PRO_0000231271
Sites
	Active site	116	1	Proton donor By similarity
	Binding site	116	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3K1L5-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 0985A1C479709B0E	FASTA	419	44,534
	10 20 30 40 50 60 MRKIIINGGK QLTGEVAVSG AKNSVVALIP ATILADDVVV LDGVPAISDV DSLVDIMETM 70 80 90 100 110 120 GAKIKRYGET LEIDPCGVKD IPMPYGKINS LRASYYFYGS LLGRYGQATL GLPGGCDLGP 130 140 150 160 170 180 RPIDLHLKAF EAMGASVSYE GDSMRLATNG KPLQGANIYM DTVSVGATIN TIIAAAKANG 190 200 210 220 230 240 RTVIENAARE PEIIDVATLL NNMGAHIRGA GTDVITIEGV KSLHGTRHQV IPDRIEAGTY 250 260 270 280 290 300 IAMAAAIGRG IKVTNVLYEH LESFIAKLDE MGVRMTVEED SIFVEEQERL KAVSIKTSPY 310 320 330 340 350 360 PGFATDLQQP LTPLLLTAEG NGSLLDTIYE KRVNHVPELA RMGANISTLG GKIVYSGPNQ 370 380 390 400 410 LSGAPVKATD LRAGAALVIA GLMAEGRTEI TNIEFILRGY SNIIEKLTSL GADIQLVEE

References

[1]

"Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial 'pan-genome'." Tettelin H., Masignani V., Cieslewicz M.J., Donati C., Medini D., Ward N.L., Angiuoli S.V., Crabtree J., Jones A.L., Durkin A.S., DeBoy R.T., Davidsen T.M., Mora M., Scarselli M., Margarit y Ros I., Peterson J.D., Hauser C.R., Sundaram J.P. , Nelson W.C., Madupu R., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sullivan S.A., Daugherty S.C., Haft D.H., Selengut J., Gwinn M.L., Zhou L., Zafar N., Khouri H., Radune D., Dimitrov G., Watkins K., O'Connor K.J., Smith S., Utterback T.R., White O., Rubens C.E., Grandi G., Madoff L.C., Kasper D.L., Telford J.L., Wessels M.R., Rappuoli R., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:13950-13955(2005) [PubMed: 16172379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27591 / A909 / CDC SS700 / Serotype Ia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000114 Genomic DNA. Translation: ABA45498.1.
RefSeq	YP_329588.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3685750.
GenomeReviews	Gene locus SAK_0966 in contig CP000114_GR.
KEGG	sak:SAK_0966.
TIGR	SAK_0966.
Phylogenomic databases
HOGENOM	Q3K1L5.
OMA	LESFICK.
Enzyme and pathway databases
BioCyc	SAGA205921:SAK_0966-MON.
Family and domain databases
HAMAP	MF_00111. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 1 hit.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA1_STRA1

Accession

Primary (citable) accession number: Q3K1L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)