Reviewed, UniProtKB/Swiss-Prot Q3K1J2 (MURA2_STRA1)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 2
    UDP-N-acetylglucosamine enolpyruvyl transferase 2
      Short name=EPT 2

Gene names

Name:	murA2
Ordered Locus Names:	SAK_0989

Organism

Streptococcus agalactiae serotype Ia [Complete proteome] [HAMAP]

Taxonomic identifier

355315 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity.
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000231272
Sites
	Active site	120	1	Proton donor By similarity
	Binding site	120	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3K1J2-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: C1FFA1F8E35102B8	FASTA	423	45,242
	10 20 30 40 50 60 MDKIIVEGGQ TQLQGQVVIE GAKNAVLPLL AATILPSQGK TLLTNVPILS DVFTMNNVVR 70 80 90 100 110 120 GLDIQVDFNC DKKEILVDAS GDILDVAPYE FVSQMRASIV VLGPILARNG HAKVSMPGGC 130 140 150 160 170 180 TIGSRPIDLH LKGLEAMGAT ITQNGGDITA QAEKLKGANI YMDFPSVGAT QNLMMAATLA 190 200 210 220 230 240 SGTTTIENAA REPEIVDLAQ LLNKMGAKVK GAGTETLTII GVDALHGTEH DVVQDRIEAG 250 260 270 280 290 300 TFMVAAAMTS GNVLVKDAIW EHNRPLISKL MEMGVEVSEE EDGIRVKADT KKLKPVTVKT 310 320 330 340 350 360 LPHPGFPTDM QAQFTALMAV VNGESTMIET VFENRFQHLE EMRRMGLQTE ILRDTAMIHG 370 380 390 400 410 420 GRALQGAPVM STDLRASAAL ILAGMVAQGQ TVVGQLTHLD RGYYQFHEKL AALGANIKRV SEA

References

[1]

"Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial 'pan-genome'." Tettelin H., Masignani V., Cieslewicz M.J., Donati C., Medini D., Ward N.L., Angiuoli S.V., Crabtree J., Jones A.L., Durkin A.S., DeBoy R.T., Davidsen T.M., Mora M., Scarselli M., Margarit y Ros I., Peterson J.D., Hauser C.R., Sundaram J.P. , Nelson W.C., Madupu R., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sullivan S.A., Daugherty S.C., Haft D.H., Selengut J., Gwinn M.L., Zhou L., Zafar N., Khouri H., Radune D., Dimitrov G., Watkins K., O'Connor K.J., Smith S., Utterback T.R., White O., Rubens C.E., Grandi G., Madoff L.C., Kasper D.L., Telford J.L., Wessels M.R., Rappuoli R., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:13950-13955(2005) [PubMed: 16172379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27591 / A909 / CDC SS700 / Serotype Ia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000114 Genomic DNA. Translation: ABA45048.1.
RefSeq	YP_329611.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3685231.
GenomeReviews	Gene locus SAK_0989 in contig CP000114_GR.
KEGG	sak:SAK_0989.
TIGR	SAK_0989.
Phylogenomic databases
HOGENOM	Q3K1J2.
OMA	VSRVYHL.
Enzyme and pathway databases
BioCyc	SAGA205921:SAK_0989-MON.
Family and domain databases
HAMAP	MF_00111. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 1 hit.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_STRA1

Accession

Primary (citable) accession number: Q3K1J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)