Q3K0T0 (CPSD_STRA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase CpsD
EC=2.7.10.2

Gene names

Name:	cpsD
Synonyms:	cpsIaD
Ordered Locus Names:	SAK_1259

Organism

Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700) [Complete proteome] [HAMAP]

Taxonomic identifier

205921 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus agalactiae › Streptococcus agalactiae serogroup Ia

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the regulation of capsular polysaccharide biosynthesis. Autophosphorylation of CpsD attenuates its activity and reduces the level of encapsulation. May be part of a complex that directs the coordinated polymerization and export to the cell surface of the capsular polysaccharide. Ref.3
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Enzyme regulation	Dephosphorylated and activated by CpsB By similarity.
Pathway	Capsule biogenesis; capsule polysaccharide biosynthesis.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the CpsD/CapB family.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation Exopolysaccharide synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	capsule polysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of catalytic activity Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW enzyme regulator activity Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Tyrosine-protein kinase CpsD		PRO_0000217238
Experimental info
	Sequence conflict	218 – 220	3	Missing in BAA82278. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q3K0T0 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: DE4CFA15A9C77C26	FASTA	232	25,402
	10 20 30 40 50 60 MTRLEIVDSK LRQAKKTEEY FNAIRTNIQF SGKENKILAI TSVREGEGKS TTSTSLALSL 70 80 90 100 110 120 AQAGFKTLLI DADTRNSVMS GTFKATGTIK GLTNYLSGNA DLGDIICETN VPRLMVVPSG 130 140 150 160 170 180 KVPPNPTALL QNAYFNKMIE AIKNIFDYII IDTPPIGLVV DAAIIANACD GFILVTQAGR 190 200 210 220 230 IKRNYVEKAK EQMEQSGSKF LGIILNKVSE SVATYGDYGD YGNYGKRDRK RK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of type-specific capsular polysaccharide biosynthesis genes of Streptococcus agalactiae type Ia." Yamamoto S., Miyake K., Koike Y., Watanabe M., Machida Y., Ohta M., Iijima S. J. Bacteriol. 181:5176-5184(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OI1 / Serotype Ia.
[2]	"Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial 'pan-genome'." Tettelin H., Masignani V., Cieslewicz M.J., Donati C., Medini D., Ward N.L., Angiuoli S.V., Crabtree J., Jones A.L., Durkin A.S., DeBoy R.T., Davidsen T.M., Mora M., Scarselli M., Margarit y Ros I., Peterson J.D., Hauser C.R., Sundaram J.P. , Nelson W.C., Madupu R., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sullivan S.A., Daugherty S.C., Haft D.H., Selengut J., Gwinn M.L., Zhou L., Zafar N., Khouri H., Radune D., Dimitrov G., Watkins K., O'Connor K.J., Smith S., Utterback T.R., White O., Rubens C.E., Grandi G., Madoff L.C., Kasper D.L., Telford J.L., Wessels M.R., Rappuoli R., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:13950-13955(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27591 / A909 / CDC SS700.
[3]	"Functional analysis in type Ia group B Streptococcus of a cluster of genes involved in extracellular polysaccharide production by diverse species of Streptococci." Cieslewicz M.J., Kasper D.L., Wang Y., Wessels M.R. J. Biol. Chem. 276:139-146(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: 515 / Serotype Ia.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028896 Genomic DNA. Translation: BAA82278.1. CP000114 Genomic DNA. Translation: ABA45962.1.
RefSeq	YP_329873.1. NC_007432.1.
3D structure databases
ProteinModelPortal	Q3K0T0.
ModBase	Search...
Protein-protein interaction databases
STRING	205921.SAK_1259.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABA45962; ABA45962; SAK_1259.
GeneID	3686251.
KEGG	sak:SAK_1259.
PATRIC	19633793. VBIStrAga82541_1203.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0489.
HOGENOM	HOG000004334.
OMA	PDEIEMI.
ProtClustDB	CLSK883944.
Enzyme and pathway databases
BioCyc	SAGA205921:GHD7-1270-MONOMER.
UniPathway	UPA00934.
Family and domain databases
InterPro	IPR025669. AAA_dom. IPR005702. EPS_synthesis. [Graphical view]
Pfam	PF13614. AAA_31. 1 hit. [Graphical view]
TIGRFAMs	TIGR01007. eps_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CPSD_STRA1

Accession

Primary (citable) accession number: Q3K0T0
Secondary accession number(s): Q93TJ2, Q9ALX5, Q9S0S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program