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Protein

Tyrosine-protein kinase CpsD

Gene

cpsD

Organism
Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of capsular polysaccharide biosynthesis. Autophosphorylation of CpsD attenuates its activity and reduces the level of encapsulation. May be part of a complex that directs the coordinated polymerization and export to the cell surface of the capsular polysaccharide.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulationi

Dephosphorylated and activated by CpsB.By similarity

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Capsule biogenesis/degradation, Exopolysaccharide synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAGA205921:GHD7-1260-MONOMER.
UniPathwayiUPA00934.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase CpsD (EC:2.7.10.2)
Gene namesi
Name:cpsD
Synonyms:cpsIaD
Ordered Locus Names:SAK_1259
OrganismiStreptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
Taxonomic identifieri205921 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002701: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Tyrosine-protein kinase CpsDPRO_0000217238Add
BLAST

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi205921.SAK_1259.

Structurei

3D structure databases

ProteinModelPortaliQ3K0T0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CpsD/CapB family.Curated

Phylogenomic databases

eggNOGiCOG0489.
HOGENOMiHOG000004334.
OMAiGKYYGKY.
OrthoDBiEOG69D3H6.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025669. AAA_dom.
IPR005702. EPS_synthesis.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13614. AAA_31. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01007. eps_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3K0T0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRLEIVDSK LRQAKKTEEY FNAIRTNIQF SGKENKILAI TSVREGEGKS
60 70 80 90 100
TTSTSLALSL AQAGFKTLLI DADTRNSVMS GTFKATGTIK GLTNYLSGNA
110 120 130 140 150
DLGDIICETN VPRLMVVPSG KVPPNPTALL QNAYFNKMIE AIKNIFDYII
160 170 180 190 200
IDTPPIGLVV DAAIIANACD GFILVTQAGR IKRNYVEKAK EQMEQSGSKF
210 220 230
LGIILNKVSE SVATYGDYGD YGNYGKRDRK RK
Length:232
Mass (Da):25,402
Last modified:November 8, 2005 - v1
Checksum:iDE4CFA15A9C77C26
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti218 – 2203Missing in BAA82278. (PubMed:10464185)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028896 Genomic DNA. Translation: BAA82278.1.
CP000114 Genomic DNA. Translation: ABA45962.1.
RefSeqiYP_329873.1. NC_007432.1.

Genome annotation databases

EnsemblBacteriaiABA45962; ABA45962; SAK_1259.
GeneIDi3686251.
KEGGisak:SAK_1259.
PATRICi19633793. VBIStrAga82541_1203.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028896 Genomic DNA. Translation: BAA82278.1.
CP000114 Genomic DNA. Translation: ABA45962.1.
RefSeqiYP_329873.1. NC_007432.1.

3D structure databases

ProteinModelPortaliQ3K0T0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi205921.SAK_1259.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA45962; ABA45962; SAK_1259.
GeneIDi3686251.
KEGGisak:SAK_1259.
PATRICi19633793. VBIStrAga82541_1203.

Phylogenomic databases

eggNOGiCOG0489.
HOGENOMiHOG000004334.
OMAiGKYYGKY.
OrthoDBiEOG69D3H6.

Enzyme and pathway databases

UniPathwayiUPA00934.
BioCyciSAGA205921:GHD7-1260-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025669. AAA_dom.
IPR005702. EPS_synthesis.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13614. AAA_31. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01007. eps_fam. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of type-specific capsular polysaccharide biosynthesis genes of Streptococcus agalactiae type Ia."
    Yamamoto S., Miyake K., Koike Y., Watanabe M., Machida Y., Ohta M., Iijima S.
    J. Bacteriol. 181:5176-5184(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: OI1 / Serotype Ia.
  2. "Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial 'pan-genome'."
    Tettelin H., Masignani V., Cieslewicz M.J., Donati C., Medini D., Ward N.L., Angiuoli S.V., Crabtree J., Jones A.L., Durkin A.S., DeBoy R.T., Davidsen T.M., Mora M., Scarselli M., Margarit y Ros I., Peterson J.D., Hauser C.R., Sundaram J.P.
    , Nelson W.C., Madupu R., Brinkac L.M., Dodson R.J., Rosovitz M.J., Sullivan S.A., Daugherty S.C., Haft D.H., Selengut J., Gwinn M.L., Zhou L., Zafar N., Khouri H., Radune D., Dimitrov G., Watkins K., O'Connor K.J., Smith S., Utterback T.R., White O., Rubens C.E., Grandi G., Madoff L.C., Kasper D.L., Telford J.L., Wessels M.R., Rappuoli R., Fraser C.M.
    Proc. Natl. Acad. Sci. U.S.A. 102:13950-13955(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27591 / A909 / CDC SS700.
  3. "Functional analysis in type Ia group B Streptococcus of a cluster of genes involved in extracellular polysaccharide production by diverse species of Streptococci."
    Cieslewicz M.J., Kasper D.L., Wang Y., Wessels M.R.
    J. Biol. Chem. 276:139-146(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: 515 / Serotype Ia.

Entry informationi

Entry nameiCPSD_STRA1
AccessioniPrimary (citable) accession number: Q3K0T0
Secondary accession number(s): Q93TJ2, Q9ALX5, Q9S0S7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 8, 2005
Last modified: January 7, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.