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Protein

Thymidylate synthase

Gene

thyA

Organism
Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei154NucleophileUniRule annotation1
Binding sitei1815,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei189dUMPUniRule annotation1
Binding sitei2785,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi133 – 134dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi178 – 181dUMPUniRule annotation4
Nucleotide bindingi219 – 221dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:SAK_1346
OrganismiStreptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
Taxonomic identifieri205921 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000006911 – 279Thymidylate synthaseAdd BLAST279

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ3K0J7
SMRiQ3K0J7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000257898
KOiK00560
OMAiWNEWEVG

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q3K0J7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKADLLFKD NITKIMSEGV FSEQARPRYK NGEMANSKYI TGAFAEYDLS
60 70 80 90 100
KGEFPITTLR PIPIKSAIKE IFWIYQDQTN DLVVLNDKYG VTYWNDWEVG
110 120 130 140 150
HTGTIGQRYG AVVKKHNIIS KLLKQLEDNP WNRRNVISLW DYEAFEETEG
160 170 180 190 200
LLPCAFQTMF DVRRVNGELY LDATLTQRSN DMLVAHHINA MQYVALQMMI
210 220 230 240 250
AKHFGWRVGK FFYFINNLHI YDNQFEQAQE LLKRQPSECN PKLVLNVPDG
260 270
TDFFDIKPDD FALVDYDPIK PQLRFDLAI
Length:279
Mass (Da):32,527
Last modified:November 8, 2005 - v1
Checksum:i865F880A56F01642
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000114 Genomic DNA Translation: ABA45602.1
RefSeqiWP_000158597.1, NC_007432.1

Genome annotation databases

EnsemblBacteriaiABA45602; ABA45602; SAK_1346
KEGGisak:SAK_1346

Similar proteinsi

Entry informationi

Entry nameiTYSY_STRA1
AccessioniPrimary (citable) accession number: Q3K0J7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health