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Protein

Adenosylhomocysteinase

Gene

ahcY

Organism
Burkholderia pseudomallei (strain 1710b)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.UniRule annotation

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.UniRule annotation

Cofactori

NAD+Note: Binds 1 NAD+ per subunit.

Pathwayi: L-homocysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylhomocysteinase (ahcY)
This subpathway is part of the pathway L-homocysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641Substrate
Binding sitei139 – 1391Substrate
Binding sitei199 – 1991Substrate
Binding sitei229 – 2291Substrate
Binding sitei233 – 2331Substrate
Binding sitei234 – 2341NADUniRule annotation
Binding sitei286 – 2861NADUniRule annotation2 Publications
Binding sitei321 – 3211NADUniRule annotation2 Publications
Binding sitei387 – 3871NADUniRule annotation2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi200 – 2023NADUniRule annotation
Nucleotide bindingi263 – 2686NADUniRule annotation
Nucleotide bindingi266 – 2672NADUniRule annotation2 Publications
Nucleotide bindingi342 – 3443NADUniRule annotation
Nucleotide bindingi342 – 3432NADUniRule annotation2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciBPSE320372:GBYB-57-MONOMER.
UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
AdenosylhomocysteinaseUniRule annotation (EC:3.3.1.1UniRule annotation)
Alternative name(s):
S-adenosyl-L-homocysteine hydrolaseUniRule annotation
Short name:
AdoHcyaseUniRule annotation
Gene namesi
Name:ahcYUniRule annotation
Ordered Locus Names:BURPS1710b_0057
OrganismiBurkholderia pseudomallei (strain 1710b)
Taxonomic identifieri320372 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000002700 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473AdenosylhomocysteinasePRO_1000024718Add
BLAST

Proteomic databases

PRIDEiQ3JY79.

Structurei

Secondary structure

1
473
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi19 – 213Combined sources
Helixi22 – 3211Combined sources
Helixi33 – 353Combined sources
Helixi37 – 459Combined sources
Turni46 – 494Combined sources
Turni51 – 544Combined sources
Beta strandi56 – 616Combined sources
Helixi65 – 7612Combined sources
Beta strandi80 – 845Combined sources
Beta strandi86 – 894Combined sources
Helixi93 – 1019Combined sources
Helixi114 – 12310Combined sources
Beta strandi135 – 1417Combined sources
Helixi142 – 15514Combined sources
Helixi157 – 1604Combined sources
Helixi166 – 17914Combined sources
Turni183 – 1864Combined sources
Helixi187 – 1904Combined sources
Beta strandi196 – 1983Combined sources
Helixi201 – 21212Combined sources
Beta strandi220 – 2223Combined sources
Helixi227 – 23913Combined sources
Helixi242 – 2509Combined sources
Beta strandi258 – 2625Combined sources
Helixi266 – 27611Combined sources
Turni277 – 2793Combined sources
Beta strandi281 – 2855Combined sources
Helixi289 – 2968Combined sources
Turni297 – 2993Combined sources
Helixi305 – 3084Combined sources
Turni309 – 3113Combined sources
Beta strandi313 – 3175Combined sources
Beta strandi319 – 3224Combined sources
Helixi327 – 3326Combined sources
Beta strandi337 – 3415Combined sources
Beta strandi343 – 3464Combined sources
Helixi352 – 3543Combined sources
Beta strandi357 – 3637Combined sources
Beta strandi366 – 3705Combined sources
Beta strandi376 – 3805Combined sources
Helixi381 – 3833Combined sources
Helixi386 – 3894Combined sources
Helixi396 – 41621Combined sources
Helixi417 – 4193Combined sources
Beta strandi422 – 4265Combined sources
Helixi429 – 44012Combined sources
Turni441 – 4444Combined sources
Helixi452 – 4587Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D64X-ray2.30A/B1-473[»]
3GLQX-ray2.30A/B1-473[»]
ProteinModelPortaliQ3JY79.
SMRiQ3JY79. Positions 12-473.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3JY79.

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000227986.
KOiK01251.
OMAiKYGCRES.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 2 hits.
PfamiPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3JY79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAAVIDSHS AQDYVVADIA LAGWGRKELN IAETEMPGLV QIRDEYKAQQ
60 70 80 90 100
PLKGARIAGS LHMTIQTGVL IETLKALGAD VRWASCNIFS TQDHAAAAIV
110 120 130 140 150
EAGTPVFAFK GESLDEYWEF SHRIFEWPNG EFANMILDDG GDATLLLILG
160 170 180 190 200
SKAEKDRSVI ARPTNEEEVA LFKSIERHLE IDGSWYSKRL AHIKGVTEET
210 220 230 240 250
TTGVHRLYQM EKDGRLPFPA FNVNDSVTKS KFDNLYGCRE SLVDGIKRAT
260 270 280 290 300
DVMIAGKIAV VAGYGDVGKG CAQSLRGLGA TVWVTEIDPI CALQAAMEGY
310 320 330 340 350
RVVTMEYAAD KADIFVTATG NYHVINHDHM KAMRHNAIVC NIGHFDSEID
360 370 380 390 400
VASTRQYQWE NIKPQVDHII FPDGKRVILL AEGRLVNLGC ATGHPSFVMS
410 420 430 440 450
NSFTNQTLAQ IELFTRGGEY ANKVYVLPKH LDEKVARLHL ARIGAQLSEL
460 470
SDDQAAYIGV SKAGPFKPDH YRY
Length:473
Mass (Da):52,201
Last modified:November 8, 2005 - v1
Checksum:i7A40ECDD0A47B596
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000124 Genomic DNA. Translation: ABA47924.1.
RefSeqiWP_004198634.1. NC_007434.1.

Genome annotation databases

EnsemblBacteriaiABA47924; ABA47924; BURPS1710b_0057.
KEGGibpm:BURPS1710b_0057.
PATRICi19232326. VBIBurPse115837_0057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000124 Genomic DNA. Translation: ABA47924.1.
RefSeqiWP_004198634.1. NC_007434.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D64X-ray2.30A/B1-473[»]
3GLQX-ray2.30A/B1-473[»]
ProteinModelPortaliQ3JY79.
SMRiQ3JY79. Positions 12-473.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ3JY79.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA47924; ABA47924; BURPS1710b_0057.
KEGGibpm:BURPS1710b_0057.
PATRICi19232326. VBIBurPse115837_0057.

Phylogenomic databases

HOGENOMiHOG000227986.
KOiK01251.
OMAiKYGCRES.

Enzyme and pathway databases

UniPathwayiUPA00314; UER00076.
BioCyciBPSE320372:GBYB-57-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ3JY79.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 2 hits.
PfamiPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAHH_BURP1
AccessioniPrimary (citable) accession number: Q3JY79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.