Reviewed, UniProtKB/Swiss-Prot Q3JWR7 (BIOD_BURP1)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	BURPS1710b_0572

Organism

Burkholderia pseudomallei (strain 1710b) [Complete proteome] [HAMAP]

Taxonomic identifier

320372 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: InterPro dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000302492
Regions
	Nucleotide binding	11 – 19	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3JWR7-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 0E3FF958C8D9D720	FASTA	240	24,908
	10 20 30 40 50 60 MSAPLSLFVT GTDTEIGKTF VSAALLHGFA RAGLRAAAMK PVAAGAYERD GAWRNEDADQ 70 80 90 100 110 120 LDAAANVALP AAIRTPFLLK APAAPHIVAA REGVALDIGT IVDAHRRACE MADVIVVEGV 130 140 150 160 170 180 GGFRVPLADT RDTADLAVAL GLPVVLVVGV RLGCISHALL TAEAIAARGL PLAGWVANRI 190 200 210 220 230 240 DPAMPFADDN VDTLRAWLER EHRAPLLGAL AHMSPPSPDA ASHALDVNLL LNALRAAAPR

References

[1]	Woods D.E., Nierman W.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000124 Genomic DNA. Translation: ABA49562.1.
RefSeq	YP_331986.1.
3D structure databases
SMR	Q3JWR7. Positions 7-213.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3JWR7.
Genome annotation databases
GeneID	3689969.
GenomeReviews	Gene locus BURPS1710b_0572 in contig CP000124_GR.
KEGG	bpm:BURPS1710b_0572.
TIGR	BURPS1710b_0572.
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	ETPEGMR.
Enzyme and pathway databases
BioCyc	BPSE320372:BURPS1710B_A0609-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BURP1

Accession

Primary (citable) accession number: Q3JWR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)