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Protein

2,3-bisphosphoglycerate-dependent phosphoglycerate mutase

Gene

gpmA

Organism
Burkholderia pseudomallei (strain 1710b)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = 3-phospho-D-glycerate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk), Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei9 – 91Tele-phosphohistidine intermediateUniRule annotation1 Publication
Binding sitei60 – 601SubstrateUniRule annotation1 Publication
Active sitei87 – 871Proton donor/acceptorUniRule annotation
Binding sitei98 – 981SubstrateUniRule annotation1 Publication
Sitei182 – 1821Transition state stabilizerUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCyciBPSE320372:GBYB-662-MONOMER.
BRENDAi5.4.2.11. 1031.
UniPathwayiUPA00109; UER00186.

Names & Taxonomyi

Protein namesi
Recommended name:
2,3-bisphosphoglycerate-dependent phosphoglycerate mutaseUniRule annotation (EC:5.4.2.11UniRule annotation)
Short name:
BPG-dependent PGAMUniRule annotation
Short name:
PGAMUniRule annotation
Short name:
PhosphoglyceromutaseUniRule annotation
Short name:
dPGMUniRule annotation
Gene namesi
Name:gpmAUniRule annotation
Ordered Locus Names:BURPS1710b_0662
OrganismiBurkholderia pseudomallei (strain 1710b)
Taxonomic identifieri320372 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000002700 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2492492,3-bisphosphoglycerate-dependent phosphoglycerate mutasePRO_0000229113Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Structurei

Secondary structure

1
249
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi13 – 164Combined sources
Helixi30 – 4516Combined sources
Beta strandi51 – 555Combined sources
Helixi59 – 7214Combined sources
Beta strandi79 – 813Combined sources
Helixi83 – 853Combined sources
Helixi91 – 933Combined sources
Helixi98 – 1058Combined sources
Helixi107 – 1159Combined sources
Helixi136 – 1383Combined sources
Helixi143 – 1453Combined sources
Helixi152 – 16514Combined sources
Helixi167 – 1726Combined sources
Beta strandi177 – 1815Combined sources
Helixi183 – 19311Combined sources
Helixi200 – 2034Combined sources
Beta strandi212 – 2165Combined sources
Beta strandi222 – 2276Combined sources
Helixi231 – 2355Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EZNX-ray2.10A/B1-249[»]
3FDZX-ray2.25A/B1-249[»]
3GP3X-ray1.50A/B/C/D1-249[»]
3GP5X-ray2.25A/B1-249[»]
3GW8X-ray1.93A/B1-249[»]
3LNTX-ray2.10A/B1-249[»]
ProteinModelPortaliQ3JWH7.
SMRiQ3JWH7. Positions 2-236.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3JWH7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 158Substrate bindingUniRule annotation1 Publication
Regioni21 – 222Substrate bindingUniRule annotation1 Publication
Regioni87 – 904Substrate bindingUniRule annotation1 Publication
Regioni114 – 1152Substrate bindingUniRule annotation1 Publication
Regioni183 – 1842Substrate bindingUniRule annotation1 Publication

Sequence similaritiesi

Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000221682.
KOiK01834.
OMAiVKNQGKK.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
HAMAPiMF_01039. PGAM_GpmA. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001345. PG/BPGM_mutase_AS.
IPR005952. Phosphogly_mut1.
[Graphical view]
PANTHERiPTHR11931. PTHR11931. 1 hit.
PfamiPF00300. His_Phos_1. 2 hits.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
TIGRFAMsiTIGR01258. pgm_1. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3JWH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKLVLIRHG ESTWNKENRF TGWVDVDLTE QGNREARQAG QLLKEAGYTF
60 70 80 90 100
DIAYTSVLKR AIRTLWHVQD QMDLMYVPVV HSWRLNERHY GALSGLNKAE
110 120 130 140 150
TAAKYGDEQV LVWRRSYDTP PPALEPGDER APYADPRYAK VPREQLPLTE
160 170 180 190 200
CLKDTVARVL PLWNESIAPA VKAGKQVLIA AHGNSLRALI KYLDGISDAD
210 220 230 240
IVGLNIPNGV PLVYELDESL TPIRHYYLGD QEAIAKAQAA VAQQGKSAA
Length:249
Mass (Da):27,893
Last modified:November 8, 2005 - v1
Checksum:iE51933D372E4896E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000124 Genomic DNA. Translation: ABA50316.1.
RefSeqiWP_004198007.1. NC_007434.1.

Genome annotation databases

EnsemblBacteriaiABA50316; ABA50316; BURPS1710b_0662.
KEGGibpm:BURPS1710b_0662.
PATRICi19233568. VBIBurPse115837_0675.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000124 Genomic DNA. Translation: ABA50316.1.
RefSeqiWP_004198007.1. NC_007434.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EZNX-ray2.10A/B1-249[»]
3FDZX-ray2.25A/B1-249[»]
3GP3X-ray1.50A/B/C/D1-249[»]
3GP5X-ray2.25A/B1-249[»]
3GW8X-ray1.93A/B1-249[»]
3LNTX-ray2.10A/B1-249[»]
ProteinModelPortaliQ3JWH7.
SMRiQ3JWH7. Positions 2-236.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA50316; ABA50316; BURPS1710b_0662.
KEGGibpm:BURPS1710b_0662.
PATRICi19233568. VBIBurPse115837_0675.

Phylogenomic databases

HOGENOMiHOG000221682.
KOiK01834.
OMAiVKNQGKK.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00186.
BioCyciBPSE320372:GBYB-662-MONOMER.
BRENDAi5.4.2.11. 1031.

Miscellaneous databases

EvolutionaryTraceiQ3JWH7.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
HAMAPiMF_01039. PGAM_GpmA. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001345. PG/BPGM_mutase_AS.
IPR005952. Phosphogly_mut1.
[Graphical view]
PANTHERiPTHR11931. PTHR11931. 1 hit.
PfamiPF00300. His_Phos_1. 2 hits.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
TIGRFAMsiTIGR01258. pgm_1. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPMA_BURP1
AccessioniPrimary (citable) accession number: Q3JWH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.