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Protein

2,3-bisphosphoglycerate-dependent phosphoglycerate mutase

Gene

gpmA

Organism
Burkholderia pseudomallei (strain 1710b)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = 3-phospho-D-glycerate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk), Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei9Tele-phosphohistidine intermediateUniRule annotation1 Publication1
Binding sitei60SubstrateUniRule annotation1 Publication1
Active sitei87Proton donor/acceptorUniRule annotation1
Binding sitei98SubstrateUniRule annotation1 Publication1
Sitei182Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BRENDAi5.4.2.11. 1031.
UniPathwayiUPA00109; UER00186.

Names & Taxonomyi

Protein namesi
Recommended name:
2,3-bisphosphoglycerate-dependent phosphoglycerate mutaseUniRule annotation (EC:5.4.2.11UniRule annotation)
Short name:
BPG-dependent PGAMUniRule annotation
Short name:
PGAMUniRule annotation
Short name:
PhosphoglyceromutaseUniRule annotation
Short name:
dPGMUniRule annotation
Gene namesi
Name:gpmAUniRule annotation
Ordered Locus Names:BURPS1710b_0662
OrganismiBurkholderia pseudomallei (strain 1710b)
Taxonomic identifieri320372 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000002700 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002291131 – 2492,3-bisphosphoglycerate-dependent phosphoglycerate mutaseAdd BLAST249

Proteomic databases

PRIDEiQ3JWH7.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Helixi13 – 16Combined sources4
Helixi30 – 45Combined sources16
Beta strandi51 – 55Combined sources5
Helixi59 – 72Combined sources14
Beta strandi79 – 81Combined sources3
Helixi83 – 85Combined sources3
Helixi91 – 93Combined sources3
Helixi98 – 105Combined sources8
Helixi107 – 115Combined sources9
Helixi136 – 138Combined sources3
Helixi143 – 145Combined sources3
Helixi152 – 165Combined sources14
Helixi167 – 172Combined sources6
Beta strandi177 – 181Combined sources5
Helixi183 – 193Combined sources11
Helixi200 – 203Combined sources4
Beta strandi212 – 216Combined sources5
Beta strandi222 – 227Combined sources6
Helixi231 – 235Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EZNX-ray2.10A/B1-249[»]
3FDZX-ray2.25A/B1-249[»]
3GP3X-ray1.50A/B/C/D1-249[»]
3GP5X-ray2.25A/B1-249[»]
3GW8X-ray1.93A/B1-249[»]
3LNTX-ray2.10A/B1-249[»]
ProteinModelPortaliQ3JWH7.
SMRiQ3JWH7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3JWH7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 15Substrate bindingUniRule annotation1 Publication8
Regioni21 – 22Substrate bindingUniRule annotation1 Publication2
Regioni87 – 90Substrate bindingUniRule annotation1 Publication4
Regioni114 – 115Substrate bindingUniRule annotation1 Publication2
Regioni183 – 184Substrate bindingUniRule annotation1 Publication2

Sequence similaritiesi

Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000221682.
KOiK01834.
OMAiVKNQGKK.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
HAMAPiMF_01039. PGAM_GpmA. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001345. PG/BPGM_mutase_AS.
IPR005952. Phosphogly_mut1.
[Graphical view]
PANTHERiPTHR11931. PTHR11931. 1 hit.
PfamiPF00300. His_Phos_1. 2 hits.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
TIGRFAMsiTIGR01258. pgm_1. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3JWH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKLVLIRHG ESTWNKENRF TGWVDVDLTE QGNREARQAG QLLKEAGYTF
60 70 80 90 100
DIAYTSVLKR AIRTLWHVQD QMDLMYVPVV HSWRLNERHY GALSGLNKAE
110 120 130 140 150
TAAKYGDEQV LVWRRSYDTP PPALEPGDER APYADPRYAK VPREQLPLTE
160 170 180 190 200
CLKDTVARVL PLWNESIAPA VKAGKQVLIA AHGNSLRALI KYLDGISDAD
210 220 230 240
IVGLNIPNGV PLVYELDESL TPIRHYYLGD QEAIAKAQAA VAQQGKSAA
Length:249
Mass (Da):27,893
Last modified:November 8, 2005 - v1
Checksum:iE51933D372E4896E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000124 Genomic DNA. Translation: ABA50316.1.
RefSeqiWP_004198007.1. NC_007434.1.

Genome annotation databases

EnsemblBacteriaiABA50316; ABA50316; BURPS1710b_0662.
KEGGibpm:BURPS1710b_0662.
PATRICi19233568. VBIBurPse115837_0675.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000124 Genomic DNA. Translation: ABA50316.1.
RefSeqiWP_004198007.1. NC_007434.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EZNX-ray2.10A/B1-249[»]
3FDZX-ray2.25A/B1-249[»]
3GP3X-ray1.50A/B/C/D1-249[»]
3GP5X-ray2.25A/B1-249[»]
3GW8X-ray1.93A/B1-249[»]
3LNTX-ray2.10A/B1-249[»]
ProteinModelPortaliQ3JWH7.
SMRiQ3JWH7.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ3JWH7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA50316; ABA50316; BURPS1710b_0662.
KEGGibpm:BURPS1710b_0662.
PATRICi19233568. VBIBurPse115837_0675.

Phylogenomic databases

HOGENOMiHOG000221682.
KOiK01834.
OMAiVKNQGKK.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00186.
BRENDAi5.4.2.11. 1031.

Miscellaneous databases

EvolutionaryTraceiQ3JWH7.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
HAMAPiMF_01039. PGAM_GpmA. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001345. PG/BPGM_mutase_AS.
IPR005952. Phosphogly_mut1.
[Graphical view]
PANTHERiPTHR11931. PTHR11931. 1 hit.
PfamiPF00300. His_Phos_1. 2 hits.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
TIGRFAMsiTIGR01258. pgm_1. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPMA_BURP1
AccessioniPrimary (citable) accession number: Q3JWH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.