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Protein

Phosphopantetheine adenylyltransferase

Gene

coaD

Organism
Burkholderia pseudomallei (strain 1710b)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.UniRule annotation

Catalytic activityi

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes CoA from (R)-pantothenate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9SubstrateUniRule annotation1
Binding sitei17ATPUniRule annotationCombined sources1 Publication1
Sitei17Transition state stabilizerUniRule annotation1
Binding sitei41SubstrateUniRule annotationCombined sources1 Publication1
Binding sitei73Substrate; via amide nitrogenUniRule annotationCombined sources1 Publication1
Binding sitei87SubstrateUniRule annotationCombined sources1 Publication1
Binding sitei98ATPUniRule annotationCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 10ATPCombined sources1 Publication5
Nucleotide bindingi88 – 90ATPUniRule annotationCombined sources1 Publication3
Nucleotide bindingi123 – 129ATPUniRule annotation1 Publication7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processCoenzyme A biosynthesis
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.3. 1031.
UniPathwayiUPA00241; UER00355.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantetheine adenylyltransferase1 PublicationUniRule annotation (EC:2.7.7.3UniRule annotation)
Alternative name(s):
Dephospho-CoA pyrophosphorylaseUniRule annotation
Pantetheine-phosphate adenylyltransferase1 PublicationUniRule annotation
Short name:
PPAT1 PublicationUniRule annotation
Gene namesi
Name:coaD1 PublicationUniRule annotation
Ordered Locus Names:BURPS1710b_0748
OrganismiBurkholderia pseudomallei (strain 1710b)
Taxonomic identifieri320372 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000002700 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000111101 – 166Phosphopantetheine adenylyltransferaseAdd BLAST166

Interactioni

Subunit structurei

Homohexamer.UniRule annotation1 Publication

Structurei

Secondary structure

1166
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi15 – 27Combined sources13
Beta strandi28 – 36Combined sources9
Helixi39 – 41Combined sources3
Helixi47 – 58Combined sources12
Beta strandi64 – 71Combined sources8
Helixi73 – 79Combined sources7
Beta strandi83 – 88Combined sources6
Helixi95 – 108Combined sources14
Beta strandi113 – 117Combined sources5
Helixi121 – 123Combined sources3
Helixi128 – 136Combined sources9
Turni142 – 144Combined sources3
Helixi147 – 160Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3K9WX-ray1.60A1-166[»]
3PXUX-ray2.10A1-166[»]
ProteinModelPortaliQ3JW91.
SMRiQ3JW91.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3JW91.

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial CoaD family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000006518.
KOiK00954.
OMAiEFQMALM.

Family and domain databases

CDDicd02163. PPAT. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00151. PPAT_bact. 1 hit.
InterProiView protein in InterPro
IPR004821. Cyt_trans-like.
IPR001980. PPAT.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF01467. CTP_transf_like. 1 hit.
PRINTSiPR01020. LPSBIOSNTHSS.
TIGRFAMsiTIGR01510. coaD_prev_kdtB. 1 hit.
TIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3JW91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVAVYPGTF DPLTRGHEDL VRRASSIFDT LVVGVADSRA KKPFFSLEER
60 70 80 90 100
LKIANEVLGH YPNVKVMGFT GLLKDFVRAN DARVIVRGLR AVSDFEYEFQ
110 120 130 140 150
MAGMNRYLLP DVETMFMTPS DQYQFISGTI VREIAQLGGD VSKFVFPSVE
160
KWLTEKVAAM AQGPSA
Length:166
Mass (Da):18,530
Last modified:November 8, 2005 - v1
Checksum:iC9CDDE2E32564B58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000124 Genomic DNA. Translation: ABA49742.1.
RefSeqiWP_004195249.1. NC_007434.1.

Genome annotation databases

EnsemblBacteriaiABA49742; ABA49742; BURPS1710b_0748.
KEGGibpm:BURPS1710b_0748.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCOAD_BURP1
AccessioniPrimary (citable) accession number: Q3JW91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 8, 2005
Last modified: July 5, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families