Reviewed, UniProtKB/Swiss-Prot Q3JVB9 (MURB_BURP1)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BURPS1710b_1072

Organism

Burkholderia pseudomallei (strain 1710b) [Complete proteome] [HAMAP]

Taxonomic identifier

320372 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000224672
Regions
	Domain	26 – 197	172	FAD-binding PCMH-type
Sites
	Active site	173	1	By similarity
	Active site	249	1	Proton donor By similarity
	Active site	345	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3JVB9-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 20ECA1AE49550D70	FASTA	349	37,588
	10 20 30 40 50 60 MPMSRPDSAV SLLPDYSLRA HNTFGFDARA RVAARIGSPG QFASLARDPR VAGLDRLVLG 70 80 90 100 110 120 GGSNVVFTRD FDGLVLLDEI RGRALVREDD GAWYVEAGGG ENWHAFVEWT LAEGMPGLEN 130 140 150 160 170 180 LALIPGTVGA APIQNIGAYG LEMKEHFASL RAVELATGEL VEFDAARCAF GYRDSFFKRD 190 200 210 220 230 240 GRGRFAIVAV TFRLPKAWTP RIGYADVARE LAARGIDARA VRARDVFDAV VAIRRAKLPD 250 260 270 280 290 300 PLALGNAGSF FKNPVIDAQA FAALRAREPD IVSYPQPDGR VKLAAGWLID RCGWKGRALG 310 320 330 340 AAAVHERQAL VLVNLGGASG ADVLALAHAI RRDVLGRFGV ELEMEPVCL

References

[1]	Woods D.E., Nierman W.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000124 Genomic DNA. Translation: ABA49889.1.
RefSeq	YP_332484.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3JVB9.
Genome annotation databases
GeneID	3691847.
GenomeReviews	Gene locus BURPS1710b_1072 in contig CP000124_GR.
KEGG	bpm:BURPS1710b_1072.
TIGR	BURPS1710b_1072.
Phylogenomic databases
HOGENOM	Q3JVB9.
OMA	IGNAGSF.
Enzyme and pathway databases
BioCyc	BPSE320372:BURPS1710B_A1131-MON.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BURP1

Accession

Primary (citable) accession number: Q3JVB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)