Reviewed, UniProtKB/Swiss-Prot Q3JTG5 (GLGB_BURP1)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	BURPS1710b_1737

Organism

Burkholderia pseudomallei (strain 1710b) [Complete proteome] [HAMAP]

Taxonomic identifier

320372 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 738	738	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000260638
Sites
	Active site	312	1	By similarity
	Active site	347	1	By similarity
	Active site	352	1	By similarity
	Active site	415	1	By similarity
	Active site	417	1	By similarity
	Active site	472	1	By similarity
	Active site	539	1	By similarity
	Active site	540	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3JTG5-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 256561F89B9B9729	FASTA	738	81,116
	10 20 30 40 50 60 MTDAPFDRAD IDALLDARHP DPFACLGPHR VGDATVVRTL LPGALRVRAI AAGGGVLGEL 70 80 90 100 110 120 RQVDPAGCFA GALPDGRERG ERPRYRLSID WPDARQDVED AYAFGTLLDE DALARFAAGD 130 140 150 160 170 180 PRAALACLGA RALDMDGVPG VRFAVWAPGA SRVSVVGDFN GWDARRHPMR LRRPWGVWEL 190 200 210 220 230 240 FVPRIGAGER YKFALRARDG AALPLKADPC ACRTEAPPRT ASIVADLDAL ERFGWHDDAW 250 260 270 280 290 300 LRARASLDLA HAPVSIYEVH PESWLRVAAE GNRSATWDEL AQRLIPYAAG MGFSHVELTP 310 320 330 340 350 360 IAEYPFGGSW GYQSLSPFAP SARFGPPEGF ARFVEHAHAA GLGVIVDWVP AHFPDDPHGL 370 380 390 400 410 420 GKFDGTALFE HADPREGWHP DWHTHVFNVG RREVGAFLIA SALAWAHRYH VDGIRVDAVA 430 440 450 460 470 480 SMLYRDYSRA AGEWVPNVYG GRENLESIAF LKHFNDTLHG PAAPPGVATF AEESTAWPGV 490 500 510 520 530 540 TAPTAEHGLG FDFKWNMGWM HDTLAYLRED PIHRRHHHDR LTFGLVYAFS ERFVLPLSHD 550 560 570 580 590 600 EVVHGKGSLA AKMPGDAWQR LANLRAYFGF MWAHPGKKLL FMGGEFAQWG EFAHDATPQW 610 620 630 640 650 660 DLLDAPAHRG VQRLVRDLNR LHAAEPALHA LDDRPAGFAW LVGDDRNNSV FAFVRRDDAG 670 680 690 700 710 720 RMLVAVCNFT PVPRTDYRLG LPAPGRWAEV LNTDGAAYGG TDAGNGGALQ ADEIPAHGER 730 WSAALRLPPL ATLWLRPA

References

[1]	Woods D.E., Nierman W.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000124 Genomic DNA. Translation: ABA50427.1.
RefSeq	YP_333138.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3JTG5.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	3689784.
GenomeReviews	Gene locus BURPS1710b_1737 in contig CP000124_GR.
KEGG	bpm:BURPS1710b_1737.
TIGR	BURPS1710b_1737.
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	PKTASIV.
Enzyme and pathway databases
BioCyc	BPSE320372:BURPS1710B_A1870-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BURP1

Accession

Primary (citable) accession number: Q3JTG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)