Reviewed, UniProtKB/Swiss-Prot Q3JRF0 (MSRB_BURP1)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase msrB
    EC=1.8.4.12
Alternative name(s):
    Peptide-methionine (R)-S-oxide reductase

Gene names

Name:	msrB
Ordered Locus Names:	BURPS1710b_2458

Organism

Burkholderia pseudomallei (strain 1710b) [Complete proteome] [HAMAP]

Taxonomic identifier

320372 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	143 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin. HAMAP MF_01400
Cofactor	Binds 1 zinc ion per subunit. The zinc ion is important for the structural integrity of the protein. HAMAP MF_01400
Sequence similarities	Belongs to the msrB Met sulfoxide reductase family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	peptide-methionine (R)-S-oxide reductase activity Inferred from electronic annotation. Source: EC peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 143

143

Peptide methionine sulfoxide reductase msrB HAMAP MF_01400

PRO_0000338617

Sites

Active site

128

1

Nucleophile By similarity

Metal binding

55

1

Zinc HAMAP MF_01400

Metal binding

58

1

Zinc HAMAP MF_01400

Metal binding

104

1

Zinc HAMAP MF_01400

Metal binding

107

1

Zinc HAMAP MF_01400

Secondary structure

1

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143

Helix Strand Turn

Details...

Helix

16 – 22

7

Helix

25 – 33

9

Turn

43 – 46

4

Beta strand

50 – 55

6

Turn

56 – 58

3

Beta strand

61 – 64

4

Helix

65 – 67

3

Beta strand

73 – 75

3

Beta strand

77 – 80

4

Beta strand

86 – 92

7

Beta strand

99 – 104

6

Turn

105 – 107

3

Beta strand

110 – 116

7

Turn

120 – 123

4

Beta strand

126 – 129

4

Helix

131 – 133

3

Beta strand

134 – 138

5

Sequences

	Sequence		Length	Mass (Da)
	Q3JRF0-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: F964A5CC23725C61	FASTA	143	16,187
	10 20 30 40 50 60 MSGDRDDPRY PYPKDDAELR RRLTPMQYEV TQHAATEPPF TGEYTDTEDA GIYHCVVCGT 70 80 90 100 110 120 ALFESGAKYH SGCGWPSYFK PIDGEVIDEK MDYTHGMTRV EVRCNQCGAH LGHVFEDGPR 130 140 DKTGLRYCIN SAALNFEAKP ERK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Woods D.E., Nierman W.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Methionine-R-sulfoxide reductase from Burkholderia pseudomallei." Seattle structural genomics center for infectious disease (SSGCID) Submitted (MAR-2008) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH ZINC IONS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP000124 Genomic DNA. Translation: ABA49928.1.

RefSeq

YP_333853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CEZ	X-ray	2.10	A/B	1-143	[»]
3CXK	X-ray	1.70	A/B	1-143	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q3JRF0.

Genome annotation databases

GeneID

3690705.

GenomeReviews

Gene locus BURPS1710b_2458 in contig CP000124_GR.

KEGG

bpm:BURPS1710b_2458.

TIGR

BURPS1710b_2458.

Phylogenomic databases

eggNOG

COG0229.

HOGENOM

HBG715255.

OMA

VEVRCNH.

Family and domain databases

HAMAP

MF_01400. MsrB.
[Tree]

InterPro

IPR002579. Methionine_sulphoxide_MsrB.
IPR011057. Mss4-like.
[Graphical view]

Gene3D

G3DSA:2.170.150.20. MsrB. 1 hit.

Pfam

PF01641. SelR. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00357. MsrB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MSRB_BURP1

Accession

Primary (citable) accession number: Q3JRF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)