Reviewed, UniProtKB/Swiss-Prot Q3JQ65 (FABH_BURP1)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase 3
    EC=2.3.1.41
Alternative name(s):
    3-oxoacyl-[acyl-carrier-protein] synthase III
    Beta-ketoacyl-ACP synthase III
      Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	BURPS1710b_2907

Organism

Burkholderia pseudomallei (strain 1710b) [Complete proteome] [HAMAP]

Taxonomic identifier

320372 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein]. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm Probable HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/acpP and fabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the fabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000070222
Regions
	Region	257 – 261	5	ACP-binding By similarity
Sites
	Active site	123	1	By similarity
	Active site	256	1	By similarity
	Active site	286	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3JQ65-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: C59750221AD45C3D	FASTA	329	34,853
	10 20 30 40 50 60 MAQSTLYSRV LGTGSYLPPD RVTNQELADR LAKDGIETSD EWIVARTGIR ARHFAAPDVT 70 80 90 100 110 120 TSDLALVAAQ RAIEAADVDP QSIDLIIVAT STPDFVFPST ACLLQNKLGI KNGGAAFDVQ 130 140 150 160 170 180 AVCSGFAYAL ATADSFIRTG QHRTALVIGA ETFSRILDFK DRTTCVLFGD GAGAVVLSAS 190 200 210 220 230 240 EEPGILGSAL HADGSYSNIL CTPGNVNRGV IAGSAFLHMD GQAVFKLAVN VLEKVAVEAL 250 260 270 280 290 300 SKAELASEQV DWLIPHQANI RIMTSTCRKL GLPQERMIVT VDEHGNTSAA SIPLALDVAV 310 320 RDGRIKRGQH VLIEGVGGGF TWGASVFRF

References

[1]	Woods D.E., Nierman W.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000124 Genomic DNA. Translation: ABA49489.1.
RefSeq	YP_334288.1.
3D structure databases
SMR	Q3JQ65. Positions 6-329.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3JQ65.
Genome annotation databases
GeneID	3690950.
GenomeReviews	Gene locus BURPS1710b_2907 in contig CP000124_GR.
KEGG	bpm:BURPS1710b_2907.
TIGR	BURPS1710b_2907.
Phylogenomic databases
eggNOG	COG0332.
HOGENOM	HBG649927.
OMA	TSADRGI.
Enzyme and pathway databases
BioCyc	BPSE320372:BURPS1710B_A3155-MONOMER.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00747. fabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_BURP1

Accession

Primary (citable) accession number: Q3JQ65

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)