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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Burkholderia pseudomallei (strain 1710b)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95S-adenosyl-L-methionineUniRule annotation1
Binding sitei120S-adenosyl-L-methionineUniRule annotation1
Binding sitei147S-adenosyl-L-methionineUniRule annotation1
Active sitei170By similarity1
Binding sitei170S-adenosyl-L-methionineUniRule annotation1
Binding sitei174SubstrateUniRule annotation1
Binding sitei206SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:BURPS1710b_3119
OrganismiBurkholderia pseudomallei (strain 1710b)
Taxonomic identifieri320372 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000002700 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002291571 – 265tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST265

Structurei

3D structure databases

ProteinModelPortaliQ3JPL2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni241 – 244Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073968.
KOiK03439.
OMAiIKLGHGV.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3JPL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHDDDPNAP GAPHDDDATA APASATRAAP AAGDDDDANP LHLRRIRSFV
60 70 80 90 100
TRAGRVSTGQ RRAIDELGPR FVIPYGSAQP DWDAIFGRRA PRVLEIGFGM
110 120 130 140 150
GASTAEIAAL RPGDDFIGVE VHEPGVGALL KLIGEQQLSN IRIIQHDAVE
160 170 180 190 200
VLAQMIAPDS LDGVHIFFPD PWHKARHHKR RLIQPPFVAQ LAAHLKPGAY
210 220 230 240 250
LHCATDWQNY AEQMLEVLSA DPSLENTAQD YAPRPGYRPV TKFERRGLRL
260
GHGVWDLVFR KKHAG
Length:265
Mass (Da):29,102
Last modified:November 8, 2005 - v1
Checksum:iD19E15399EE03B40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000124 Genomic DNA. Translation: ABA49298.1.
RefSeqiWP_004527577.1. NC_007434.1.

Genome annotation databases

EnsemblBacteriaiABA49298; ABA49298; BURPS1710b_3119.
KEGGibpm:BURPS1710b_3119.
PATRICi19238802. VBIBurPse115837_3252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000124 Genomic DNA. Translation: ABA49298.1.
RefSeqiWP_004527577.1. NC_007434.1.

3D structure databases

ProteinModelPortaliQ3JPL2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA49298; ABA49298; BURPS1710b_3119.
KEGGibpm:BURPS1710b_3119.
PATRICi19238802. VBIBurPse115837_3252.

Phylogenomic databases

HOGENOMiHOG000073968.
KOiK03439.
OMAiIKLGHGV.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_BURP1
AccessioniPrimary (citable) accession number: Q3JPL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.