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Q3JPL2 (TRMB_BURP1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BURPS1710b_3119
OrganismBurkholderia pseudomallei (strain 1710b) [Complete proteome] [HAMAP]
Taxonomic identifier320372 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229157

Regions

Region241 – 2444Substrate binding By similarity

Sites

Active site1701 By similarity
Binding site951S-adenosyl-L-methionine By similarity
Binding site1201S-adenosyl-L-methionine By similarity
Binding site1471S-adenosyl-L-methionine By similarity
Binding site1701S-adenosyl-L-methionine By similarity
Binding site1741Substrate By similarity
Binding site2061Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3JPL2 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: D19E15399EE03B40

FASTA26529,102
        10         20         30         40         50         60 
MIHDDDPNAP GAPHDDDATA APASATRAAP AAGDDDDANP LHLRRIRSFV TRAGRVSTGQ 

        70         80         90        100        110        120 
RRAIDELGPR FVIPYGSAQP DWDAIFGRRA PRVLEIGFGM GASTAEIAAL RPGDDFIGVE 

       130        140        150        160        170        180 
VHEPGVGALL KLIGEQQLSN IRIIQHDAVE VLAQMIAPDS LDGVHIFFPD PWHKARHHKR 

       190        200        210        220        230        240 
RLIQPPFVAQ LAAHLKPGAY LHCATDWQNY AEQMLEVLSA DPSLENTAQD YAPRPGYRPV 

       250        260 
TKFERRGLRL GHGVWDLVFR KKHAG 

« Hide

References

[1]Woods D.E., Nierman W.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1710b.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000124 Genomic DNA. Translation: ABA49298.1.
RefSeqYP_334491.1. NC_007434.1.

3D structure databases

ProteinModelPortalQ3JPL2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING320372.BURPS1710b_3119.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA49298; ABA49298; BURPS1710b_3119.
GeneID3688754.
KEGGbpm:BURPS1710b_3119.
PATRIC19238802. VBIBurPse115837_3252.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycBPSE320372:GBYB-3119-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BURP1
AccessionPrimary (citable) accession number: Q3JPL2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 8, 2005
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways