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Reviewed, UniProtKB/Swiss-Prot Q3JPJ6 (URE1_BURP1)

Last modified November 3, 2009. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha
Gene names
Name: ureC
Ordered Locus Names: BURPS1710b_3135
OrganismBurkholderia pseudomallei (strain 1710b) [Complete proteome] [HAMAP]
Taxonomic identifier320372 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

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Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953

Cofactor

Binds 2 nickel ions per subunit By similarity.

Pathway

Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953

Subunit structure

Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamylation allows a single lysine to coordinate two nickel ions By similarity.

Sequence similarities

Belongs to the urease family.

Contains 1 urease domain.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Nickel
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processurea metabolic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionnickel ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

urease activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 564564Urease subunit alpha HAMAP MF_01953
PRO_0000234149

Regions

Domain126 – 564439Urease

Sites

Active site3171Proton donor By similarity
Metal binding1311Nickel 2 By similarity
Metal binding1331Nickel 2 By similarity
Metal binding2141Nickel 1; via carbamate group By similarity
Metal binding2141Nickel 2; via carbamate group By similarity
Metal binding2431Nickel 1 By similarity
Metal binding2691Nickel 1 By similarity
Metal binding3571Nickel 2 By similarity
Binding site2161Substrate By similarity

Amino acid modifications

Modified residue2141N6-carboxylysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q3JPJ6-1 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: C07451F00BCB5533

FASTA56460,209
        10         20         30         40         50         60 
MSRRAYAEMY GPTTGDRIRL ADTELLIEVE RDHTLYGEEV KFGGGKVIRD GMGQSQLPAA 

        70         80         90        100        110        120 
DVADTVITNA VILDHWGIVK ADIAIKHGRI AAIGKAGNPD IQPGVTIAIG AATEIIAGEG 

       130        140        150        160        170        180 
LIVTAGGIDT HIHFISPQQI DEALASGVTT MIGGGTGPAT GTNATTCTPG PWHMERMLQA 

       190        200        210        220        230        240 
ADGWPINLGF LGKGNASRPQ PLVEQIEAGA IGLKLHEDWG TTPAAIDNCL TVADDTDTQV 

       250        260        270        280        290        300 
AIHTDTLNEA GFVEATVAAF KGRTIHTYHT EGAGGGHAPD ILKVCGEANV LPSSTNPTRP 

       310        320        330        340        350        360 
YTINTLDEHL DMLMVCHHLD PSIAEDLAFA ESRIRRETIA AEDILHDLGA LSMLSSDSQA 

       370        380        390        400        410        420 
MGRVGEVIIR TWQTAHKMKV QRGALTGDGA RNDNFRAKRY VAKYTINPAL THGIAHEVGS 

       430        440        450        460        470        480 
IEPGKWADLV LWEPAFFGVK PAMIVKGGMI AVAQMGDPNA SIPTPQPVHY REMFATRGGA 

       490        500        510        520        530        540 
LARTSLTFVS QLALDAGIGA RYGLAKRLVP VRGCRTVTKR DMIHNAWQPA IRVDPETYDV 

       550        560 
VADGALLTCE PAAVLPMAQR YFLF

« Hide

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References

[1]Woods D.E., Nierman W.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000124 Genomic DNA. Translation: ABA49103.1.
RefSeqYP_334507.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ3JPJ6.

Genome annotation databases

GeneID3690369.
GenomeReviewsGene locus BURPS1710b_3135 in contig CP000124_GR.
KEGGbpm:BURPS1710b_3135.
TIGRBURPS1710b_3135.

Phylogenomic databases

HOGENOMQ3JPJ6.
OMASHIHFIC.

Family and domain databases

HAMAPMF_01953.
[Tree]
InterProIPR006680. Amidohydro_1.
IPR011612. Urease_alpha_N.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR017952. Urease_asu_core.
IPR017950. Urease_asu_CS.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSPR01752. UREASE.
TIGRFAMsTIGR01792. urease_alph. 1 hit.
PROSITEPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameURE1_BURP1
AccessionPrimary (citable) accession number: Q3JPJ6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: November 8, 2005
Last modified: November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents