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Q3JNS7 (RUVC_BURP1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:BURPS1710b_3405
OrganismBurkholderia pseudomallei (strain 1710b) [Complete proteome] [HAMAP]
Taxonomic identifier320372 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group

Protein attributes

Sequence length180 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence caution

The sequence ABA50071.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 180180Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000225127

Sites

Metal binding71Magnesium By similarity
Metal binding661Magnesium By similarity
Metal binding1381Magnesium By similarity
Metal binding1411Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3JNS7 [UniParc].

Last modified March 7, 2006. Version 2.
Checksum: 4F8BE45AC2D2C35A

FASTA18018,648
        10         20         30         40         50         60 
MRILGIDPGL RVTGFGVIDV SGHQLAYVAS GVIKTPTADL PTRLGTIYDG VSTLIREHTP 

        70         80         90        100        110        120 
DQAAIEKVFV NVNPQSTLLL GQARGAAICG LVSGGLPVAE YTALQLKQAV VGYGRATKEQ 

       130        140        150        160        170        180 
MQEMVARLLS LSGRPGTDAA DALGMAICHA HGGNTLNTLG GIAPALAKKG LRVRRGRLVG 

« Hide

References

[1]Woods D.E., Nierman W.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1710b.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000124 Genomic DNA. Translation: ABA50071.1. Different initiation.
RefSeqYP_334776.2. NC_007434.1.

3D structure databases

ProteinModelPortalQ3JNS7.
SMRQ3JNS7. Positions 2-154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING320372.BURPS1710b_3405.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA50071; ABA50071; BURPS1710b_3405.
GeneID3688533.
KEGGbpm:BURPS1710b_3405.
PATRIC19239388. VBIBurPse115837_3544.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Enzyme and pathway databases

BioCycBPSE320372:GBYB-3405-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_BURP1
AccessionPrimary (citable) accession number: Q3JNS7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 7, 2006
Last modified: April 16, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families