Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Malate dehydrogenase

Gene

mdh

Organism
Burkholderia pseudomallei (strain 1710b)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93SubstrateUniRule annotation1
Binding sitei99SubstrateUniRule annotation1
Binding sitei106NADUniRule annotation1
Binding sitei113NADUniRule annotation1
Binding sitei132SubstrateUniRule annotation1
Binding sitei163SubstrateUniRule annotation1
Active sitei188Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 18NADUniRule annotation7
Nucleotide bindingi130 – 132NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:BURPS1710b_A0795
OrganismiBurkholderia pseudomallei (strain 1710b)
Taxonomic identifieri320372 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000002700 Componenti: Chromosome II

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002943802 – 327Malate dehydrogenaseAdd BLAST326

Proteomic databases

PRIDEiQ3JKE9.

Structurei

Secondary structure

1327
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Helixi16 – 26Combined sources11
Turni27 – 31Combined sources5
Beta strandi37 – 42Combined sources6
Helixi45 – 47Combined sources3
Helixi48 – 59Combined sources12
Beta strandi66 – 73Combined sources8
Helixi75 – 79Combined sources5
Beta strandi83 – 87Combined sources5
Helixi99 – 120Combined sources22
Beta strandi126 – 129Combined sources4
Beta strandi131 – 133Combined sources3
Helixi134 – 143Combined sources10
Helixi150 – 152Combined sources3
Beta strandi153 – 155Combined sources3
Helixi158 – 172Combined sources15
Helixi176 – 178Combined sources3
Beta strandi183 – 186Combined sources4
Beta strandi193 – 195Combined sources3
Helixi206 – 210Combined sources5
Helixi214 – 218Combined sources5
Helixi220 – 225Combined sources6
Helixi227 – 235Combined sources9
Helixi240 – 256Combined sources17
Beta strandi263 – 268Combined sources6
Helixi272 – 274Combined sources3
Beta strandi280 – 288Combined sources9
Beta strandi291 – 294Combined sources4
Helixi302 – 319Combined sources18
Turni320 – 322Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D5TX-ray2.51A/B/C/D1-327[»]
ProteinModelPortaliQ3JKE9.
SMRiQ3JKE9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3JKE9.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220953.
KOiK00024.
OMAiQYPDAGQ.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3JKE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKPAKRVAV TGAAGQIAYS LLFRIANGDL LGKDQPVILQ LLDLPQAQAA
60 70 80 90 100
VKGVVMELDD CAFPLLAGVV ITDDPKVAFK DADVALLVGA RPRSKGMERK
110 120 130 140 150
DLLSANAEIF TVQGAALNEV ASRDVKVLVV GNPANTNAYI AMKSAPDLPK
160 170 180 190 200
KNFTAMLRLD HNRALSQLAA KSGKPVASIE KLAVWGNHSP TMYPDFRFAT
210 220 230 240 250
AEGESLLKLI NDDVWNRDTF IPTVGKRGAA IIEARGLSSA ASAANAAIDH
260 270 280 290 300
VRDWVLGTNG KWVTMGIPSD GSYGIPEDII YGVPVICENG EYKRVEGLEI
310 320
DAFSREKMDG TLAELLEERD GVAHLLK
Length:327
Mass (Da):35,015
Last modified:November 8, 2005 - v1
Checksum:i2E919ABA6D3F67B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000125 Genomic DNA. Translation: ABA52700.1.
RefSeqiWP_004187735.1. NC_007435.1.

Genome annotation databases

EnsemblBacteriaiABA52700; ABA52700; BURPS1710b_A0795.
KEGGibpm:BURPS1710b_A0795.
PATRICi19241993. VBIBurPse115837_4835.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000125 Genomic DNA. Translation: ABA52700.1.
RefSeqiWP_004187735.1. NC_007435.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D5TX-ray2.51A/B/C/D1-327[»]
ProteinModelPortaliQ3JKE9.
SMRiQ3JKE9.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ3JKE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA52700; ABA52700; BURPS1710b_A0795.
KEGGibpm:BURPS1710b_A0795.
PATRICi19241993. VBIBurPse115837_4835.

Phylogenomic databases

HOGENOMiHOG000220953.
KOiK00024.
OMAiQYPDAGQ.

Miscellaneous databases

EvolutionaryTraceiQ3JKE9.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_BURP1
AccessioniPrimary (citable) accession number: Q3JKE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.