Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q3JKE9

- MDH_BURP1

UniProt

Q3JKE9 - MDH_BURP1

Protein

Malate dehydrogenase

Gene

mdh

Organism
Burkholderia pseudomallei (strain 1710b)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931SubstrateUniRule annotation
    Binding sitei99 – 991SubstrateUniRule annotation
    Binding sitei106 – 1061NADUniRule annotation
    Binding sitei113 – 1131NADUniRule annotation
    Binding sitei132 – 1321SubstrateUniRule annotation
    Binding sitei163 – 1631SubstrateUniRule annotation
    Active sitei188 – 1881Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 187NADUniRule annotation
    Nucleotide bindingi130 – 1323NADUniRule annotation

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciBPSE320372:GBYB-4593-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:BURPS1710b_A0795
    OrganismiBurkholderia pseudomallei (strain 1710b)
    Taxonomic identifieri320372 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
    ProteomesiUP000002700: Chromosome II

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 327326Malate dehydrogenasePRO_0000294380Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi320372.BURPS1710b_A0795.

    Structurei

    Secondary structure

    1
    327
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 127
    Helixi16 – 2611
    Turni27 – 315
    Beta strandi37 – 426
    Helixi45 – 473
    Helixi48 – 5912
    Beta strandi66 – 738
    Helixi75 – 795
    Beta strandi83 – 875
    Helixi99 – 12022
    Beta strandi126 – 1294
    Beta strandi131 – 1333
    Helixi134 – 14310
    Helixi150 – 1523
    Beta strandi153 – 1553
    Helixi158 – 17215
    Helixi176 – 1783
    Beta strandi183 – 1864
    Beta strandi193 – 1953
    Helixi206 – 2105
    Helixi214 – 2185
    Helixi220 – 2256
    Helixi227 – 2359
    Helixi240 – 25617
    Beta strandi263 – 2686
    Helixi272 – 2743
    Beta strandi280 – 2889
    Beta strandi291 – 2944
    Helixi302 – 31918
    Turni320 – 3223

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3D5TX-ray2.51A/B/C/D1-327[»]
    ProteinModelPortaliQ3JKE9.
    SMRiQ3JKE9. Positions 3-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ3JKE9.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000220953.
    KOiK00024.
    OMAiNCLIASK.
    OrthoDBiEOG6PP9Q2.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q3JKE9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKPAKRVAV TGAAGQIAYS LLFRIANGDL LGKDQPVILQ LLDLPQAQAA    50
    VKGVVMELDD CAFPLLAGVV ITDDPKVAFK DADVALLVGA RPRSKGMERK 100
    DLLSANAEIF TVQGAALNEV ASRDVKVLVV GNPANTNAYI AMKSAPDLPK 150
    KNFTAMLRLD HNRALSQLAA KSGKPVASIE KLAVWGNHSP TMYPDFRFAT 200
    AEGESLLKLI NDDVWNRDTF IPTVGKRGAA IIEARGLSSA ASAANAAIDH 250
    VRDWVLGTNG KWVTMGIPSD GSYGIPEDII YGVPVICENG EYKRVEGLEI 300
    DAFSREKMDG TLAELLEERD GVAHLLK 327
    Length:327
    Mass (Da):35,015
    Last modified:November 8, 2005 - v1
    Checksum:i2E919ABA6D3F67B3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000125 Genomic DNA. Translation: ABA52700.1.
    RefSeqiYP_335954.1. NC_007435.1.

    Genome annotation databases

    EnsemblBacteriaiABA52700; ABA52700; BURPS1710b_A0795.
    GeneIDi3693272.
    KEGGibpm:BURPS1710b_A0795.
    PATRICi19241993. VBIBurPse115837_4835.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000125 Genomic DNA. Translation: ABA52700.1 .
    RefSeqi YP_335954.1. NC_007435.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3D5T X-ray 2.51 A/B/C/D 1-327 [» ]
    ProteinModelPortali Q3JKE9.
    SMRi Q3JKE9. Positions 3-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 320372.BURPS1710b_A0795.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA52700 ; ABA52700 ; BURPS1710b_A0795 .
    GeneIDi 3693272.
    KEGGi bpm:BURPS1710b_A0795.
    PATRICi 19241993. VBIBurPse115837_4835.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000220953.
    KOi K00024.
    OMAi NCLIASK.
    OrthoDBi EOG6PP9Q2.

    Enzyme and pathway databases

    BioCyci BPSE320372:GBYB-4593-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q3JKE9.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01517. Malate_dehydrog_2.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Woods D.E., Nierman W.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1710b.

    Entry informationi

    Entry nameiMDH_BURP1
    AccessioniPrimary (citable) accession number: Q3JKE9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3