Q3JFK2 (ASPD_BURP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable L-aspartate dehydrogenase
EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	BURPS1710b_A2501

Organism

Burkholderia pseudomallei (strain 1710b) [Complete proteome] [HAMAP]

Taxonomic identifier

320372 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › Burkholderia pseudomallei

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP-Rule MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP-Rule MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP-Rule MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP-Rule MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	NAD binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Probable L-aspartate dehydrogenase HAMAP-Rule MF_01265		PRO_1000067298
Sites
	Active site	224	1	By similarity
	Binding site	128	1	NAD; via amide nitrogen By similarity
	Binding site	194	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3JFK2 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 4FB855C9F3D2D311	FASTA	271	27,761
	10 20 30 40 50 60 MRNAHAPVDV AMIGFGAIGA AVYRAVEHDA ALRVAHVIVP EHQCDAVRGA LGERVDVVSS 70 80 90 100 110 120 VDALACRPQF ALECAGHGAL VDHVVPLLKA GTDCAVASIG ALSDLALLDA LSNAADAGGA 130 140 150 160 170 180 TLTLLSGAIG GIDALAAARQ GGLDEVRYIG RKPPLGWLGT PAEAICDLRA MAAEQTIFEG 190 200 210 220 230 240 SARDAAQLYP RNANVAATIA LAGVGLDATR VCLIADPAVT RNVHRIVARG AFGEMSIEMS 250 260 270 GKPLPDNPKT SALTAFSAIR ALRNRASHCV I

References

[1]	Woods D.E., Nierman W.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1710b.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000125 Genomic DNA. Translation: ABA53762.1.
RefSeq	YP_337651.1. NC_007435.1.
3D structure databases
ProteinModelPortal	Q3JFK2.
ModBase	Search...
Protein-protein interaction databases
STRING	320372.BURPS1710b_A2501.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABA53762; ABA53762; BURPS1710b_A2501.
GeneID	3693319.
KEGG	bpm:BURPS1710b_A2501.
PATRIC	19245755. VBIBurPse115837_6708.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1712.
HOGENOM	HOG000206326.
KO	K06989.
OMA	PKTSYLA.
ProtClustDB	PRK13303.
Enzyme and pathway databases
BioCyc	BPSE320372:GBYB-6299-MONOMER.
UniPathway	UPA00253; UER00456.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_01265. NadX.
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_BURP1

Accession

Primary (citable) accession number: Q3JFK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 8, 2005
Last modified:	May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program