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Protein

Glutamate--tRNA ligase 1

Gene

gltX1

Organism
Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi98ZincUniRule annotation1
Metal bindingi100ZincUniRule annotation1
Metal bindingi125ZincUniRule annotation1
Metal bindingi127ZincUniRule annotation1
Binding sitei239ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 1UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 1UniRule annotation
Short name:
GluRS 1UniRule annotation
Gene namesi
Name:gltX1UniRule annotation
Ordered Locus Names:Noc_0264
OrganismiNitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107)
Taxonomic identifieri323261 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus
Proteomesi
  • UP000006838 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002373771 – 469Glutamate--tRNA ligase 1Add BLAST469

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323261.Noc_0264.

Structurei

3D structure databases

ProteinModelPortaliQ3JEF2.
SMRiQ3JEF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionUniRule annotationAdd BLAST11
Motifi236 – 240"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252722.
KOiK01885.
OMAiENIHHAI.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3JEF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVRTRFAPS PTGYLHVGGV RTALFSWLYA RKHGGRFILR IEDTDRERSS
60 70 80 90 100
MESVNAILEG MTWLGLEYDE GPFYQTNRFS RYREIIEQLL ASNHAYLCYC
110 120 130 140 150
TREELASMRA EQMARKEKPR YDGRCRERHS PREGVSPVVR FKSPLEGRVV
160 170 180 190 200
IRDLVRGMVI FQNNELDDLV LARTDGTPTY NLTVVVDDMD MGMTHVIRGD
210 220 230 240 250
DHLNNTPRQS NIFYALGKEP PVYAHVPMIL GPDRQRLSKR HGAVSVMNYR
260 270 280 290 300
EAGYLPEALL NYLVRLGWSH GDQEIFSVAE MIKLFDIEDV NQSASTFNSE
310 320 330 340 350
KLLWLNQQYI KNSAPEHIAH HLSWHLGQLG IDPSKGPDLA AVVKAQQERS
360 370 380 390 400
KTLLEMAHNS APFYREFEAY EETAARKHLN ASVLGPLRDL RERFKEAQSW
410 420 430 440 450
VAPALHEIIL ATVESHHLKL GKLAQPLRVA IMGRPISPPI DVTLELMGQA
460
TTLARIDRAL AWIDHRSNA
Length:469
Mass (Da):53,503
Last modified:November 8, 2005 - v1
Checksum:iF103C1F1005D1D0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000127 Genomic DNA. Translation: ABA56794.1.
RefSeqiWP_002812825.1. NC_007484.1.

Genome annotation databases

EnsemblBacteriaiABA56794; ABA56794; Noc_0264.
KEGGinoc:Noc_0264.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSYE1_NITOC
AccessioniPrimary (citable) accession number: Q3JEF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 8, 2005
Last modified: June 7, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families