Reviewed, UniProtKB/Swiss-Prot Q3JEF2 (SYE1_NITOC)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Noc_0264

Organism

Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) [Complete proteome] [HAMAP]

Taxonomic identifier

323261 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Nitrosococcus

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00022
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000237377
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022
	Motif	236 – 240	5	"KMSKS" region HAMAP MF_00022
Sites
	Metal binding	98	1	Zinc By similarity
	Metal binding	100	1	Zinc By similarity
	Metal binding	125	1	Zinc By similarity
	Metal binding	127	1	Zinc By similarity
	Binding site	239	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3JEF2-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: F103C1F1005D1D0E	FASTA	469	53,503
	10 20 30 40 50 60 MTVRTRFAPS PTGYLHVGGV RTALFSWLYA RKHGGRFILR IEDTDRERSS MESVNAILEG 70 80 90 100 110 120 MTWLGLEYDE GPFYQTNRFS RYREIIEQLL ASNHAYLCYC TREELASMRA EQMARKEKPR 130 140 150 160 170 180 YDGRCRERHS PREGVSPVVR FKSPLEGRVV IRDLVRGMVI FQNNELDDLV LARTDGTPTY 190 200 210 220 230 240 NLTVVVDDMD MGMTHVIRGD DHLNNTPRQS NIFYALGKEP PVYAHVPMIL GPDRQRLSKR 250 260 270 280 290 300 HGAVSVMNYR EAGYLPEALL NYLVRLGWSH GDQEIFSVAE MIKLFDIEDV NQSASTFNSE 310 320 330 340 350 360 KLLWLNQQYI KNSAPEHIAH HLSWHLGQLG IDPSKGPDLA AVVKAQQERS KTLLEMAHNS 370 380 390 400 410 420 APFYREFEAY EETAARKHLN ASVLGPLRDL RERFKEAQSW VAPALHEIIL ATVESHHLKL 430 440 450 460 GKLAQPLRVA IMGRPISPPI DVTLELMGQA TTLARIDRAL AWIDHRSNA

References

[1]

"Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707." Klotz M.G., Arp D.J., Chain P.S.G., El-Sheikh A.F., Hauser L.J., Hommes N.G., Larimer F.W., Malfatti S.A., Norton J.M., Poret-Peterson A.T., Vergez L.M., Ward B.B. Appl. Environ. Microbiol. 72:6299-6315(2006) [PubMed: 16957257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000127 Genomic DNA. Translation: ABA56794.1.
RefSeq	YP_342324.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3JEF2.
Genome annotation databases
GeneID	3706338.
GenomeReviews	Gene locus Noc_0264 in contig CP000127_GR.
KEGG	noc:Noc_0264.
NMPDR	fig|323261.3.peg.870.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3JEF2.
OMA	MARYDAN.
Enzyme and pathway databases
BioCyc	NOCE323261:NOC_0264-MON.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_NITOC

Accession

Primary (citable) accession number: Q3JEF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)